[Objective] To dicuss the application of contraray treatment from“Yel ow Emperor’s Internal Classics”in gynecological diseases, hoping to guide the clinic. [Method] By reviewing the articles concerning contrary treatment in “True Large Theory.Yel ow Emperor’s Internal Classics”, take clinical recipe cases as examples, such as stimulating the menstrual flow and water circulation for long menstruation; dispel ing wind to relieve exterior syndrome for night sweat in menstruation;dismounting for morbid leucorrhea;reinforcing spleen for pregnant digestion;tonifying Qi for pregnant constipation;warm drugs for sore throat, etc. Meanwhile, it sums up the application rules of the contrary treatment in gynecological diseases. [Result] The gynecological dis-eases can be cured by contrary therapy based on differentiation of signs, i.e.treating diarrhea with purgative,treating obstruction with tonics, treating heat with warm, al with good cure effect proved by practice.[Conclusion] To treat gynecological diseases with contrary therapy is the demonstration of search-ing for primary cause in treatment;we shal further dig out, inherit and develop it and make it better serve the clinic.

Objective] To explore Ma Dazheng professor Chinese medicine treatment for the treatment of leukorrhagia. [Methods] Ma ’s treatment of gynecological disease discusses perspectives and clinical experience in terms of academic pathogenesis, treatment, therapies, their basic prescription compatibility and subtraction of the common CD features are summarized.[Results] Ma Dazheng professor applied lishi, tonifying spleen qi, kidney spleen, heat detoxification treatment, heat treatment and water as the main syndromes, pixu(spleen deficient), spleen kidney Yang deficiency type and the wet poison down, had clinical curative effect. [Conclusion] Ma Dazheng professor according to the characteristics of the patients in the process of treating leukorrhagia, syndrome differentiation and treatment, obtained the clinical accuracy and good curative effect, the gynecological clinical diagnosis and treatment had a good guiding significance, worthy of our in-depth study and understanding.

Objective To study the traditional Chinese medicine treatment in headache in menstruation of Ma Dazheng professor. [Methods]The paper expounds Professor Ma’s experience in treating headache in menstruation by analyzing the etiology, pathology, summaring the clinical thoughts and citing some typical cases. [Results]After the syndrome differentiation methods fitting which were from Ma Dazheng professor of TCM, good resulets have been achieved in the each model headache of dialectical classification. [Conclusion]The causes of headache have a lot, but according to the characteristics of the patients with the clinical syndrome differentiation, choosing precision wil get very good curative effect, it is a good guide for clinical treatment in a headach in menstruation.

Objective:To explore the clinical effects of acupoint sticking combined with Bushen Yiqi and Guchong Antai decoction in the treatment of threatened abortion with kidney deficiency.Methods:From June 2018 to February 2019, 80 patients with threatened abortion and kidney deficiency type in Wenzhou Hospital of Traditional Chinese Medicine were selected and divided into observation group and control group according to the random digital table method, with 40 cases in each group.The control group was given progesterone injection, while the observation group received acupoint sticking combined with Bushen Yiqi and Guchong Antai decoction on the basis of the control group.Both two groups were treated for 2 weeks.Before and after treatment, the improvement of clinical symptoms, the levels of sex hormones[serum progesterone(P), estradiol(E 2), human chorionic gonadotropin(β-HCG), progesterone-induced blockade factor(PIBF)] and lymph immune cells were recorded and compared in the two groups, and the clinical efficacy was evaluated. Results:After treatment, the vaginal bleeding, lumbosacral soreness, bleeding time, burnout symptoms and total scores in the two groups were all decreased significantly compared with those before treatment( t≥29.780, all P<0.05), which of the observation group decreased more significantly( t=11.655, 10.864, 8.898, 1.910, 17.415, all P<0.055). The total effective rate in the observation group was 95.00%(38/40), which was significantly higher than 70.00%(28/40) in the control group(χ 2=5.854, P<0.05). After treatment, the levels of P, E 2, β-HCG and PIBF increased significantly in the two groups( t≥5.507, all P<0.05), but the increasing degrees in the observation group were more significant( t=6.358, 16.297, 112.692, 3.262, all P<0.05). After treatment, the levels of CD 4+ and NK in the observation group were significantly lower than those before treatment( t=12.538, 4.520, all P<0.05), and the level of CD 8+ in the observation group was significantly higher than that before treatment( t=6.982, P<0.05), but the above indicators in the control group before and after treatment had no statistically significant differences( t=0.769, 1.594, 1.292, all P>0.05). Conclusion:Acupoint sticking combined with Bushen Yiqi and Guchong Antai decoction can significantly improve the clinical symptoms of threatened abortion women, which has better clinical effects, and its mechanism may be related to the regulation of hormone levels and chronic inflammatory status in pregnant women.

OBJECTIVETo investigate the effect of astragaloside IV (As IV) on the activation of rennin-angiotensin system in rats with pressure-overload induced cardiac hypertrophy.

METHODLeft ventricle hypertrophy was induced by abdominal aorta banding between bilateral renal aortas for 12 weeks. Rats were given astragaloside IV 1.0 mg x kg(-1) and 3.3 mg x kg(-1) for 12 weeks, respectively. After treatment, the left ventricular mass index (LVMI)was calculated by morphometry methods. Plasma and cardiac tissue angiotensin II, and plasma aldosterone were measured by ELISA method. Gene expressions of ACE, AT1 and AT2 in cardiac tissue were detected by real time PCR. Protein expressions of AT1 and AT2 in cardiac tissue were detected by Western blot.

RESULTCompared with model rats, LVMI was decreased by astragaloside IV treatment. Biochemical results indicated that the contents of angiotensin II in plasma and cardiac tissue as well as aldosterone in plasma were all increased in abdominal aorta banding rats comparing with sham-operated rats, then, decreased by astragaloside IV treatment. Gene expressions of cardiac ACE was downregulated by astragaloside IV, however, gene and protein expressions of cardiac AT2 were upregulated by astragaloside IV. Both elevated gene and protein expressions of AT1 were not attenuated by astragaloside IV.

CONCLUSIONExcessive activated rennin-angiotensin system in rats with pressure-overload induced cardiac hypertrophy is inhibited by astragaloside IV treatment.

Aldosterone ; blood ; Angiotensin II ; blood ; metabolism ; Animals ; Blood Pressure ; physiology ; Cardiomegaly ; drug therapy ; Enzyme-Linked Immunosorbent Assay ; Hypertrophy, Left Ventricular ; drug therapy ; metabolism ; Male ; Peptidyl-Dipeptidase A ; genetics ; Polymerase Chain Reaction ; Rats ; Rats, Sprague-Dawley ; Receptor, Angiotensin, Type 1 ; genetics ; Receptor, Angiotensin, Type 2 ; genetics ; Renin-Angiotensin System ; drug effects ; Saponins ; therapeutic use ; Triterpenes ; therapeutic use