1.A novel method for easy and reliable endotracheal intubation in the mouse
Lihui LIU ; Lingyu YE ; Yuanming TAN ; Zhaoqian LIU ; Hatton WILLIAM ; Dayue DUAN
Journal of Chinese Physician 2001;0(08):-
Objective To explore a safe and efficient method for endotracheal intubation in mice.Methods A small longitudinal incision was made in the middle of the neck in rats.After the trachea was exposed,the rats were undergone endotracheal intubation and the operation was completed in about 1 min.Results The success rate of intubation was 100% in over 1300 cases.Conclusion The whole procedure can be finished by one person without extra equipment and special mouse position.This novel and efficient method can significantly improve the success rate in using mice as animal models for in vivo studies.
2.Chrysophanol protects against doxorubicin-induced cardiotoxicity by suppressing cellular PARylation.
Jing LU ; Jingyan LI ; Yuehuai HU ; Zhen GUO ; Duanping SUN ; Panxia WANG ; Kaiteng GUO ; Dayue Darrel DUAN ; Si GAO ; Jianmin JIANG ; Junjian WANG ; Peiqing LIU
Acta Pharmaceutica Sinica B 2019;9(4):782-793
The clinical application of doxorubicin (DOX) in cancer chemotherapy is limited by its life-threatening cardiotoxic effects. Chrysophanol (CHR), an anthraquinone compound isolated from the rhizome of L., is considered to play a broad role in a variety of biological processes. However, the effects of CHR׳s cardioprotection in DOX-induced cardiomyopathy is poorly understood. In this study, we found that the cardiac apoptosis, mitochondrial injury and cellular PARylation levels were significantly increased in H9C2 cells treated by Dox, while these effects were suppressed by CHR. Similar results were observed when PARP1 activity was suppressed by its inhibitors 3-aminobenzamide (3AB) and ABT888. Ectopic expression of PARP1 effectively blocked this CHR׳s cardioprotection against DOX-induced cardiomyocyte injury in H9C2 cells. Furthermore, pre-administration with both CHR and 3AB relieved DOX-induced cardiac apoptosis, mitochondrial impairment and heart dysfunction in Sprague-Dawley rat model. These results revealed that CHR protects against DOX-induced cardiotoxicity by suppressing cellular PARylation and provided critical evidence that PARylation may be a novel target for DOX-induced cardiomyopathy.