Objective To evaluate the clinical effect of transcatheter ethanol embolization of bilateral uterine artery as a treatment for fibromyoma. Methods Twenty one women with uterine fibromyoma underwent femoral artery puncture and transcatheter uterine artery embolization. Free flow bilateral artery embolization with ethanol were performed on 16 cases, three women undergoing unilateral embolization, and gelatin sponge were used in 2 cases. Results All cases were observed with a follow up of 3 to 64 months (mean 25 months) after the treatment. Nineteen women infused with ethanol were reported noticeable symptomatic improvement, including fifteen women with complete resolution of symptoms. Two women embolized with sponge exhibited clinical response at 2 and 5 months after the procedure, respectively. The marked diminution of tumor size (40%-90%) was sonographically demonstrated. No severe complications were discovered in all 21 cases. Conclusion Uterine artery embolization represented a promising new method of treating fibroids. It had safe and good clinical results. Further investigation would be required to assess its long term effect.

Objective To observe the clinical curative effect of transcatheter arterial chemoembolization (TACE) in combination with 30% percutaneous acetic acid injection (PAI) under B-ultrasonic guidance to cure primary massive hepatocellular carcinoma. Methods Twinty three patients with intermediate or advanced stage of massive hepatocellular carcinomas were undergone transcatheter arterial chemoembolization. After 15-20 days, 30% percutaneous acetic injection was done for 2-3 times under B-ultrasonic guidance. Results After 2-3 periods of treatment, a significant difference (P

The present paper is aimed to explore the biological osteoinductive activity of recombinant human bone morphogenetic protein 9 (rhBMP-9) by various biological technologies. In this study, we firstly obtained hBMP-9 cDNA by PCR and inserted it into vector pcDNA4/His Max to reconstruct hBMP-9 eukaryotic expression vector pcDNA4/His Max-BMP-9. Recombinant Chinese hamster ovary (rCHO) cell line expressing high-level rhBMP-9 was reconstructed by co-transfecting the expression vectors pcDNA4/His* Max-hBMP-9 and plasmid pSV2-dhfr into dihydrofolate reductase (dhfr)-deficient CHO cells and the subsequent gene amplification by the methotrexate. We finally obtained a monoclonal cell line expressing the highest level protein. We purified the medium after culturing the highest-producing monoclonal by Ni-NTA His-Bind Resin columns and concentrated to by a Centricon 50 at 4 degrees C and stored at 70 degrees C until it was used. Western blot and SDS-PAGE analyses showed a specific band of about 32kD in pro-region lane and a specific band of about 50kD in pro-region complex lane. Biological activities of rhBMP-9 were tested by colorimetric determination and histochemical staining of Alkaline Phosphatase (ALP) Activity, osteocalcin and oesteopontin for C3H10 T1/2 cells, which were stimulated culture by different concentration (20, 50, 100 microg/mL) of rhBMP-9. The results showed that the rhBMP-9 could induce osteogenic differentiation of mesenchymal stem cells (MSCs) in vitro, and were proportional to the amount. This study can provide experimental data for further tests in vivo and clinical applications.
Animals ; CHO Cells ; Cricetinae ; Cricetulus ; Growth Differentiation Factor 2 ; biosynthesis ; isolation & purification ; pharmacology ; Humans ; Osteogenesis ; drug effects ; Recombinant Proteins ; biosynthesis ; isolation & purification ; pharmacology ; Transfection

OBJECTIVETo establish quality control criteria for medicinal herb Cajanus cajan based on the determination of longistylin A and longistylin C, two bioactive and specific stilbenes of the plant.

METHODLongistylin A and longistylin C were obtained from the leaves of C. cajan by silica gel column chromatography and identified as marker compounds of this plant by spectroscopic analysis. A RP-HPLC method was established to determine the two compounds.

RESULTLongistylin A and longistylin C were well separated on a Thermo BDS Hypersil C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase methanol-water (8:2), and showed good linearity in the range of 0.00288 - 0.0576 microg and 0.0112 - 0.224 microg, respectively. The average recoveries were 98.9% and 97.2% with RSD of 2.4% and 2.2% for these two compounds, respectively.

CONCLUSIONThe established analysis method is simple and accurate, whicn can be used for quality control of C. cajan.

Cajanus ; chemistry ; Chromatography, High Pressure Liquid ; methods ; Diethylstilbestrol ; analysis ; isolation & purification ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Plant Leaves ; chemistry ; Plants, Medicinal ; chemistry