OBJECTIVE:To investigate the pharmacotherapy for Parkinson disease(PD)and the factors influencing the choice of the treatment schemes for the PD patients.METHDOS:A total 269 patients with PD were assigned to 3 groups:Group I(Dopamine Agonist or Antan),Group Ⅱ(L-dopa),and Group Ⅲ(L-dopa plus Dopamine Agonist).The possible relative factors such as patients' age and sex,pathogenetic condition,educational level,occupation and income etc were analyzed statistically.RESULTS:The P value of the likelihood ratio test concerning the investigated factors such as age,sex,pathogenetic condition,education level,occupation and income were all less than 0.05 among patients treated with different kinds of therapy.The logistic regression analysis showed that all the six factors mentioned above had influence on the choice of therapy.CONCLUSION:The PD patient's selection of therapy was affected by his age and sex,pathogenetic condition,education level,occupation and income.

Objective: Strengthen the school education of full-time medical junior college students,and to explore the teaching method of them. Methods:The teachers should adjust the teaching method in view of the teaching difficult points of full-time medical junior college students, to teach students in accordance with their aptitude:1)To go into the inner world of students, in-depth understand the characteristics of students;2)Enrich the teaching method and stimulate students' interest in learning; 3)To use the PBL teaching method properly; 4)To change the traditional examination methods;5)To strengthen the training of Teachers. Results:Self-confidence of the medical college students is strengthened, learning interest is improved, and learning effect is improved obviously through the teaching methods above. Conclusion:It can provide a good reference for teaching of medical junior college students through the methods of strengthen the school education of medical junior college students, and cultivate more qualified excellent medical talents for basic medical and health institutions.

Objective To discuss the effect of expanded deltopectoral skin flaps in repairing scar contracture deformity of facio-cervical .Methods Twenty-four patients with scar contracture deformity of faciocervical were included into the study .We expanded the deltopectoral skin flap with dilator .8-10 weeks later ,the deltopectoral skin flap were transferred after resection of faciocervical scar .3-4 weeks later ,the pedicles were cut off ,and then the remaining wound were repaired .Results They had not exudation .Ex-pander exposure ,local hematoma and local infection respectively were 1 ,3 ,4 cases ,all did not affect operation effect by symptomatic treatment ,and obtain good postoperative effect .Deltopectoral flap was for 27 cases ,blood supply obstruction low temperature and skin flap necrosis respectively were 4 ,2 ,1 cases ,all get good healing after corresponding .Followed up for 0 .5 year ,all flaps were survived well ,no ectropion or mental cervical adhesion ,its color and texture were similar with surrounding normal skin appearance , so they were got high satisfaction .Conclusion Application of expanded deltopectoral skin flaps to repair scar contracture deformity of faciocervical can achieve good effect and may be an ideal method to repair ,which is worthy of clinical application .

HIV-1 integrase (IN) is a key enzyme for the viral replication. The protein-protein interaction (PPI) between HIV-1 IN and a cellular cofactor lens epithelium-derived growth factor (LEDGF/p75) is a validated target for anti-HIV drug discovery. In order to build the platform for screening inhibitor against PPI between IN and LEDGF/p75, the vector containing the LEDGF/p75 protein cDNA was constructed and expressed in Escherichia coli and the function of the LEDGF/p75 protein was assayed. The LGDGF/p75 encoding gene optimized according to the preference codon usage of E. coli, was synthesized and cloned into the expression vector pGEX-4T-1 to form a recombined plasmid, then transformed into host cell E. coli BL21 (DE3). The recombined clones were identified and confirmed by BamH I/Sal I digestion and sequencing, the successfully recombined plasmid in the host cell was induced by IPTG and the condition of the expression was optimized. The expressed protein was purified by the Ni2+ affinity chromatography column and SDS-PAGE was used to analyze the molecular weight and specificity. In addition, ELISA assay was used to analyze the function of the recombinant protein. The recombinant LGDGF/p75 was soluble, and expressed highly and stably in E. coli. The protein was proved to enhance HIV-1 IN strand transfer activity in vitro by ELISA. It will be helpful to build the platform of screening inhibitors against PPI between IN and LEDGF/p75.

Objective To explore the effect of ginseng combined with transplantation of bone marrow mesenchymal stem cells(BMSCs)on functional recovery of rats with spinal cord injury(SCI). Methods Forty-eight female Wistar rats were randomly assigned to following four groups(n=12 per group):the sham operation group was treated by opening the vertebral lamina and exposing spinal cord without SCI;the SCI model group was reproduced by using improved Allen bump method and afterwards no treatment was given;methyl prednisolone(MP)group was treated by MP pulse treatment after SCI,including intravenous injection of MP 30 mg/kg immediately after SCI and 4 hours later the same injection was repeated,and then the same intravenous injection 2 times daily,3 days in total;The ginseng+BMSCs group was treated by orally taking ginseng ultra-microgranules 300 mg/kg after SCI,twice a day for 20 days and BMSCs 5μL(concentration 1×107 cell/μL)transplantation was carried out in SCI region on the 7th day after SCI. In the above 4 groups,the ethological observation(BBB scores)was done regularly and on the 30th day after operation,silver staining was applied to investigate the changes of spinal cord,and neuro-electrophysiological tests including somatosensory evoked potential(SEP)and motor evoked potential(MEP)were performed. Results In sham operation group,after surgery the movement of both hind limbs became temporarily sluggish and on the 7th day their functions recovered to approximately normal. In SCI model group,after injury paralysis of both hind limbs occurred,while in the MP group and ginseng+BMSCs group,different degrees of functional recovery of the injured limbs developed,and the recovery in ginseng + BMSCs group was more significant. Compared with sham operation group,after surgery the BBB score was reduced markedly at various time points in SCI model group;compared to the SCI model group,the BBB scores in MP and ginseng+BMSCs groups were increased significantly,especially more remarkable in ginseng+BMSCs group(all P<0.05),and beginning from the 12th day after operation,the difference became obvious (5.23±1.22 vs. 3.61±1.03, P<0.05). Histological detection showed that in sham operation group,the structure of spinal cord was complete,neurons distributed evenly in the gray matter and a large number of silver staining positive nerve fibers paralleled to each other and arranged regularly;in SCI model group,fragmented construction was present and the defects of gray and white matters were prominent. Compared with the SCI model group, the extents of tissue necrosis in MP and ginseng + BMSCs groups were ameliorated. The neuro-electrophysiological tests demonstrated that in SCI model group,the loss of normal wave form occurred. Compared with SCI model group,in MP and ginseng+BMSCs groups,after treatment latent periods(ms)were shortened prominently in different degrees of SEP and MEP,and their peak-to-peak values(mV)were increased obviously;the improvement in potential in ginseng+BMSCs group was greater than that in MP group〔SEP:latent period(ms):3.31±0.36 vs. 4.66±0.33, peak-to-peak value(mV):0.10±0.01 vs. 0.05±0.01,MEP:latent period(ms):3.40±0.13 vs. 4.24±0.31, peak-to-peak value(mV):41.12±0.56 vs. 16.46±2.83,all P<0.05〕. Conclusion A combined treatment of ginseng and BMSCs transplantation can effectively promote the recovery of neural function for rats with SCI.

Purpose To study X-ray and CT findings of pulmonary infection in AIDS. Methods We evaluate 10 cases who have chest abnormalities. The all patients have X-ray and CT examination. Results Imaging of pulmonary tuberculosis in AIDS, including infiltrative imaging and diffuse patchy imaging, enlarged mediastinum lymph nodes and extra chest enlarged lymph nodes. The imaging of legionella species is infiltrative imaging. Carinii pneumonia appearances diffuse infiltrative and interstitial abnormalities. Conclusion Pulmonary tuberculosis, legionella species and carinii pneumonia are common infections in AIDS. The patchy, consolidations, diffused diseases and enlarged lymph nodes are important imaging appearances.

Objective o explore intraperitoneal human fluorine safe release chemotherapy after radical surgery on the prognosis of patients with gastric cancer.Methods One hundred gastric cancer patients who were treated in Jinshan Hospital Affiliated to Fudan University from June 2011 to September 2012 were selected and divided into observation groups (gastrectomy + Human fluorine An chemotherapy) and control group (simple gastrectomy) according to a random number table method.Biochemical index before treatment and 7 d after treatment,complication occurrence and survival rate of two groups were compared.Results There were no statistically significant differences in terms of WBC,PLT,IBIL,Cr and other biochemical markers before and after treatment between the two groups (P＞0.05).There were statistically significant differences in terms of chemical peritonitis,intestinal obstruction,fistula,intestinal perforation and other complications between two groups (P＞0.05).The 6 months,1 year,2-year survival rates of control group were 87.50％,80.00％,62.50％,of observation group were 96.67％,90.00％,78.33％,the differences were significant (x2 =6.108,8.796,4.579;P=0.014,0.003,0.032).The 6 months,1 year,2-year free survival rates of control group were 75.00％,55.00％,35.00％,of observation group were 93.33％,86.67％,70.00％,the differences were significant (x2 =3.902,6.635,5.203;P =0.045,0.010,0.021).Conclusion Intraoperative intraperitoneal chemotherapy sustained radical surgery in patients with no adverse effects on the stomach,can effectively improve the postoperative survival rates,worthy of promotion.

Objective To study the biological and clinical features of familial testicular tumor. Methods 2 cases of familial testicular tumor were reviewed of which one was between father son and the other uncle nephew. Results Of the case between father son,the son was testicular teratoid cancer and father seminoma.Of the other case,both were testicular seminoma. Conclusions Familial testicular tumor presents specific clinical and biological features which help its diagnosis.

Objective To investigate the sensitization to HLA antigens and relationship between sensitization and allograft function after liver transplantation. Methods The panel reactive antibody (PRA) in serum was determined by using LAT ELISA kit in 15 recipients before and after liver transplantation. Results Moderate sensitization was detected in one of 15 cases before transplantation. The patient experienced one episode of mild rejection, which was reversed by treatment with Methylprednisolone. PRA in this patient maintained positive 3 months posttransplantation. The remaining 14 patients had negative PRA (

[Objective]To investigate the clinical value of different magnetic resonance(MR) pulse sequences in the diagnosis of bone contusion.[Method]Forty-five patients with bone contusion underwent magnetic resonance imaging(MRI).All cases underwent computed radiography(CR),digital radiography(DR)or computed tomography(CT)and no bone fracture were revealed.Complications included swelling、pain and limit activity.The sagittal,coronal and transversal scanning were performed by Siemens Novus1.5T magnetic resonance imaging.The body coil were used on spines and knee coil on knees.MR pulse sequences included spin echo T1WI,Turbo spin echo T2WI,fl-2d T2WI and fat suppressed sequence T2WI.[Result]In 45 cases with bone contusion,44 cases were revealed on spin echo T1WI(97.7%),37 cases on Turbo spin echo T2WI(82.2%),24 cases on fl-2d T2WI(75%),45 cases on fat suppressed sequence T2WI(100%).The fat suppressed sequence T2WI was superior to TSE T2WI(