Objective: To find out the effect of mineralization condition medium on bone marrow stromal cells(MSCs) proliferation,differentiation and the secretion of extracellular matrix. Methods: MSCs of the third passage were induced by minerlization condition medium (?=10%FBS,10 nmol/L dexamethasone,50 mg/L L-vitamin C and 10 mmol/L ?-sodium glycerophosphate in DMEM). The control cells were cultured in DMEM with ?=10% FBS. The cells were observed by inverted microscope. The proliferation status and alkaline phosphatase level of cells were investigated with MTT assay. Mineralization potential was studied by Von-Kossa staining. TypeⅠ,type Ⅲ collagen and BMP synthesis were examined by immunohistochemistry stains. Results:The proliferation of MSCs in mineralization condition medium was decreased, but ALP level was increased(P

Objective:To investigate the feasibility of marrow stromal cells/ceramic bovine bone/hydrogel in the repair of autogenous calvarial defects. Methods:The complex of ceramic bovine bone,hydrogel,rhBMP-2,TGF-? and marrow stromal cells(MSCs) induced by minerlization inducing medium was prepared and applied in the repair of autogenous calvarial defects in 8 SD rats(group A).Ceramic bovine bone /MSCs was used as control in another 8 SD rats(group B). 4 and 8 weeks after operation 4 rats in each group were sacrificed, the osteogenesis was examined by HE staining,modified Mallory's trichrome staining and image analysis.Results:4 weeks after operation, osseous plerosis can be seen in both groups, the quantity of bone-like tissues formation(?m~2) in group A and group B was 6 813.09?96.32 and 3 839.25?104.52(P

Objective To study the effect of the combination of rivaroxaban and Xuesaitong injection on deep venous thrombosis (DVT) of lower extremity after spinal surgery.Methods The clinical data of 90 patients undergoing spinal surgery from May 2013 to May 2015 were retrospective analyzed.The patients were divided into two groups randomly: the treatment group and control group.Forty-five patients in treatment group were treated with rivaroxaban and Xueshuantong injection from 1 st day after surgery to one week after off-bed activity, and forty-five patients in control group were treated with rivaroxaban alone.The vessels of lower extremity were detected by doppler ultrasonography pre-surgery, 7-10d and 3 months post-surgery, then the rates of DVT and pulmonary embolism were statistically analysed.Results The DVT rate in treatment group was 8.89%(4 cases), which was significantly lower than 24.4% (11 cases) in control group (P<0.05).Conclusion The anticogulant effect of the combination of rivaroxaban and Xuesaitong injection is stronger than rivaroxaban alone.

Objective: To investigate the effect of ceramic bovine bone (CBB) combined with hydrogel complex (CBB/HG), ?-tricalcium phosphate (?-TCP) combined with hydrogel complex(?-TCP/HG) on the osteogenesis of bone marrow stromal cells (MSCs) in vitro. Methods: MSCs(induced by 50 mg/L ascorbic acid,10 -8 mol/L dexamethasone and 0.01 mol/L ?-sodium glycerylphosphate) were cultured on CBB/HG , ?-TCP/HG,CBB and ?-TCP respectively. 5 and 10 days after set up, marrow stromal cell attachment and morphology were observed by scanning electronic microscope, the proliferation of the cells was evaluated by cell counting, Alkaline phosphatase(ALP) activity was tested,typeⅠcollagen synthesis were examined by immunohistochemistry stainning. Results: On day 5 and 10 the marrow stromal cells on CBB/HG and on ?-TCP/HG were significantly more than those on CBB or ?-TCP. TypeⅠcollagen synthesis and ALP activity of the cells on CBB/HG and ?-TCP /HG were not different but significantly higher than those on CBB or ?-TCP. Conclusion: CBB/HG and ?-TCP /HG may stimulate bone marrow stromal cell proliferation, differentiation and extracellular matrix synthesis.

Objective To evaluate the dose distribution of target volume and normal tissues with different treatment planning such as three dimensional conformal radiotherapy(3DCRT) ,simplified intensity modulated radiotherapy(sIMRT) ,and intensity modulated radiotherapy (IMRT) for patients with radically resected rectal cancer. Methods Ten male patients with stage Ⅱ and Ⅲ rectal cancer after radical resection (Dixon surgery) were enrolled in this study. 3-field or 5-field 3DCRT,slMRT and 5-field or 7-field IMRT plans were performed for each patient. The dose distributions of target volume and normal tissues,conformal index(CI) and heterogeneous index(HI) were analyzed using the dose-volume histogram(Dvit). The prescription dose was 50 Gy in 25 fractions. Results The CI for PTV of IMRT and sIMRT was superior to 3DCRT. Conversely,the HI for PTV of 3DCRT was superior to sIMRT and IMRT. sIMRT and IMRT can protect the organs at risk better than 3DCRT. The mean of total MU for 3DCRT3f,3DCRT5f, sIMRT, IMRT5f and IMRTT7f was 482±13,504±11,455±42,841±36 and 884±46, respectively. Conclusions Comparing with 3DCRT plans and IMRT plans, sIMRT plan was the optimal plan for clinical practice. All of the three radiotherapy techniques can protect the rectal stump and anal canal well with the prescription dose of 50 Gy.

Objective To commission a Mobetron intra-operative mobile accelerator and analyze the characteristics of its electron beams. Methods The dosimetrie characteristics of the electron beams genera-ted by Mobetron accelerator were measured and compared with those generated by conventional accelerator (Primus, Siemens). M oberton accelerator can generate electron beams of nominal energies of 4,6,9 and 12 MeV. The measurement items were as followings : percentage depth dose perpendicular to water phantom sur-face and beam profiles parallel to water phantom surface, output factors, applicator leakage, electron beam at-tenuation made by lead blocks,and machine output calibration. The measurement devices included a three-dimensional ( 3 D) water scanning phantom, an electrometer, a 0.6 cm3 Farmer ionization chamber, a parallel-plate ionization chamber and solid water slabs. During measurement, all applicators of different tilt angles and diameters were attached to the machine head,and their ends were adjusted to be tangent to the phantom surface. Results Except for the 12 MeV,skin dose for all energies was no more than 90%. The skin dose was higher for Mobetron accelerator electron beams than for regular electron beams. The Dmax depth in water for a 10 cm flat applicator were 0.7,1.3,2.0 and 2.2 cm for the 4 energies,respectively. The depths of 90% dose were 1.0,1.8,2.7 and 3.6 cm, respectively. The selected flat applicator was just 1 cm larger than the tumor bed. But for the beveled applicators,the field flatness and symmetry became worse,and con-sequently,the applicator size had to be selected based on the isodose distribution. The leakage dose at 1 cm outside the applicator was 1.2% ,5.1%, 10.0% and 9.1%, respectively. The lead thickness for full block was 1.5,3.0,4.5 and 6.0 mm,respectively. Conclusions Through the commissioning of Mobetron accel-erator, the machine characteristics are understood, and the data for clinical implementation and routine quality assurance are acquired.

OBJECTIVE:To discuss the application of biomedical fibrin glue in laparoscopic hepatectomy(LH).METHO-DS:40 patients undergoing LH were randomly divided into biomedical fibrin glue group(trial group) and control group:the patients in trial group underwent LH,transection of liver by ultrasound knife,with the transected liver surface sprayed with biomedical fibrin glue guided by laparoscope;the control group underwent routine LH only.The postoperative blood loss and bile leakage were observed by T-tube drainage.RESULTS:In the biomedical fibrin glue group,the operations for all the patients were successful,the postoperative recovery was smooth,the postoperative drainage volume,RBC in the drained fluid,level of hemoglobin and length of hospital stay were all significantly lower than in the control group(P

Objective Studies on IMRT technique demonstrates that the improved dose homogeneities throughout the breast as well as reduction in dose to the heart and the ipsi-and control-lateral lungs. Methods Based on the physical principle of rolling tangential technique, a treatment plan for a patient has been planned by using Pinnacle aperture-based inversion software. The rolling tangential IM-beam was pre-determined at each pre-selected gantry angle with normally angle separation of 5?, 10? or 15?. Each beam's width was so chosen that the inner-and outer-edge of the beam should be tangential to the inner chest wall and target outer surface respectively. For the starting and ending tangential beam, a pair of opposing beam was designed. The beam's orientation was suggested to be reversed to its opposite angle when the tangential beam's gantry angle reached 90? or 270?. Each beam's shape and size were adjusted on its BEV map. For each tangential beam, 7 sub-segment beams were chosen for each tangential beam. Then, planning system was allowed to do optimization according to the clinical dose prescriptions. Results The calculated dose distributions in target(CTV), left & right lungs, and heart showed that the volume received the prescribed dose 50?Gy V_ 50 was 0.95; The volume in target enclosed with 95%, 105%, 110%, 115% isodose lines V_ 95% , V_ 105% , V_ 110% , V_ 115% was 0.98, 0.84, 0.53, 0.18, respectively. The volume of V_ 10 ,V_ 20 , V_ 30 , V_ 40 V_ 50 , in the lungs and heart were significantly decreased when compared to the current IMRT techniques published in the literature(Table 1).Conclusions The rolling tangential IM-segment irradiation technique for the breast developed by the author is able to offer not only a better dose distribution and better dose conformity throughout the target, but also reduce significantly the dose to the lungs and heart, with the patient's set up and localization process being very simple. The treatment planning for individual patient is optimal in any treatment planning system with Aperture-Optimization inverse software. This technique is certainly to offer more benefit to the patient if some measures are taken to limit or to follow the movement of the chest wall during irradiation.

Glomerulonephritis and rheumatic heart disease are usually inferred as immune complex disease caused by infections, but the pathogenesis of rheumatic heart disease is still not clear. In this experiment, we infected 38 rabbits with cercariae of S. japonicum and de-monstrated the histopathological changes of endocarditis, myocarditis and perivasculitis in the heart tissues of 33(86.8%) infected rabbits. Thrombosis was also found in heare vessels, but adult schistosomes or eggs were not found in the heart tissues. Direct and ndirect immune enzyme staining studies showed the presence of schistosome-specific antigenst and host antibodies in the heart tissues, suggesting that the histopathological changes in ithe infected rabbit hearts were resulted from the deposition of immune complexes and th secondary events pf host autoimmune reactions. In this paper, correlative clinical cases were reviewed and the possibility of heart disease in human schistosomiasis caused by immune mechanism was discussed. We hope that the above findings might broaden our understanding of the pathogenesis of rheumatic heart disease, myocardiopathy and cardio-vascular diseases (Plates 1-3).

To find anti-hypertensive lead drug, angiotensin converting enzyme (ACE) inhibitory peptides were synthesized and their effects on inhibiting ACE activity were investigated. ACE inhibitory peptides were synthesized via Fmoc solid-phase synthesis, isolated and purified through reversed phase high-performance liquid chromatography (RP-HPLC), and identified by mass spectrometry. A RP-HPLC analysis method was used to test ACE inhibitory activity in vitro of these ACE inhibitory peptides. Six octapeptides were successfully synthesized, and the analytical results of mass spectrum were consistent with their theoretically calculated data. Among these synthetic octapeptides, the anti-SARS (severe acute respiratory syndromes) octapeptide had the most obvious ACE inhibitory activity with an IC50 value of 3.4 x 10(-5) mol x L(-1). So octapeptide AVLQSGFR-OH (anti-SARS peptide) was found to be the strongest candidate for potential development as an anti-hypertensive drug and had the implication of further study.