Objective:To investigate the infiltration of CD45RO+ T cells and CD68+ cells in the lesions of non-neoplastic epithelial disorder and in vulva cancer.Methods:The infiltration of CD45RO+ T cells and CD68+ cells was detected with S-P immunohistochemistry in the lesions of 20 non-neoplastic epithelial disorders,including vulvar squamous cell hyperplasia(SH) and vulvar lichen sclerosus(LS)) and vulvar cancer,respectively.The vulvar skins from 10 healthy individuals were selected as the control.Results:The infiltration of CD45RO+ T cells and CD68+ cells in healthy vulvar skin was very low and significantly lower than that in SH,LS and vulvar cancer(P0.05).The number of both cells were significantly higher than that in SH,poorly vulvar cancer and early stage of LS(P0.05).The infiltration of CD68+ cells was shown in a positive correlation with CD45RO+ T cells in the different lesion of vulva,the correlation coefficient was 0.742(P

OBJECTIVETo study clinicopathological features, diagnosis, differential diagnosis of oral Langerhans cell histiocytosis (LCH), retrospective clinicopathologic study was carried on and a variety of immune phenotype were detected.

METHODSThe clinicopathological features of 29 cases of oral LCH were analyzed. The immunohistochemical staining of S-100 protein, CD1a, CD83 and Ki-67 were used in above cases by immunohistochemical streptavidin-biotin peroxidase (SP) and Elivison two-step method. Statistical analysis was adopted for the results.

RESULTSOf the 29 cases of LCH, the expression of S-100 protein and CD1a were positive in 24 cases and negative in 5 cases, so 5 cases were excluded from the diagnosis of LCH. Among 24 cases of LCH, 15 patients were male and 9 were female. The median age was 7.50 years. 14 lesions were in the mandible, 5 were in the maxilla and 5 involved the mandible and maxilla. 9 cases were in stage I, 13 in stage II and 2 in stage III, according to Bartnick classification. Immunohistochemistry showed all 24 cases staining for S-100 protein and CD1a were positive. Comparing with maxillofacial lesions involved soft tissue, Ki-67 positive rate was lower and CD83 positive rate was higher in maxillofacial single bone lesion.

CONCLUSIONThe immunohistochemical staining of S-100 protein and CD1a are important for the diagnosis of LCH. Maxillofacial bone single LCH might have lower proliferative activity and a higher state of maturity. Maxillofacial LCH involved soft tissue might have a higher proliferative activity and a lower state of maturity.

Antigens, CD1 ; Diagnosis, Differential ; Female ; Histiocytosis, Langerhans-Cell ; Humans ; Immunohistochemistry ; Male ; Mandible ; Maxilla ; Retrospective Studies ; S100 Proteins

Objective: To investigate the relationship between plasma levels of procalcitonin(PCT), C-reactive protein(CRP) and tumor necrosis factor-alpha(TNF-α) and postoperative intestinal obstruction in patients with colorectal cancer. Methods: From December 2014 to December 2017, 60 patients with colorectal cancer and 60 patients who had no intestinal obstruction after operation in the Third Hospital of Shanxi Datong Coal Mine Group Limited Company were selected.The patients were divided into intestinal obstruction group and non-intestinal obstruction group.All the patients received blood examination, and the levels of PCT, CRP and TNF-α were measured.The levels of PCT, CRP and TNF-α were compared between the obstruction group and the non-intestinal obstruction group before operation, 1 day after operation and 5 days after operation.The levels of PCT, CRP and TNF-α in patients with intestinal obstruction were compared at different time points after operation.According to the outcome on the 5th day after operation, the patients in the intestinal obstruction group were divided into effective group and ineffective group, and the levels of PCT, CRP and TNF-α were compared between the effective group and the ineffective group.Pearson correlation analysis was used to analyze the correlation between the levels of PCT, CRP and TNF-α with postoperative intestinal obstruction in patients with colorectal cancer. Results: At preoperative 1d and postoperative 1d, the PCT, CRP and TNF-α levels between the intestinal obstruction group and non-intestinal obstruction group had no statistically significant differences(preoperation t=0.706, 0.459, 0.249, postoperative 1 day t=0.874, 0.434, 1.460, all P>0.05). At 5 days after surgery, the levels of PCT[(1.89±0.53)ng/mL], CRP[(10.06±2.50)mg/L], TNF-α[(1.95±0.59)ng/L]in the intestinal obstruction group were higher than those in the non-intestinal obstruction group[(1.05±0.32)ng/mL, (7.27±2.06)mg/L, (1.15±0.28)ng/L, t=10.510, 6.671, 9.489, all P<0.05]. The levels of PCT, CRP and TNF-α at the fifth day after intestinal obstruction were higher than those at the first day after operation (t=12.762, 6.708, 6.973, all P<0.05). The levels of PCT[(2.13±0.48)ng/mL], CRP[(11.24±2.37)mg/L], TNF-α[(2.16±0.40)ng/L] of the ineffective group were higher than those of the effective group[(1.64±0.31)ng/mL, (8.86±1.65)mg/L, (1.77±0.32)ng/L, t=4.757, 4.507, 4.052, all P<0.05]. Correlation analysis showed that PCT, CRP and TNF-α levels were closely related to postoperative intestinal obstruction in colorectal cancer patients, and all of them were positively correlated(r=0.892, 0.817, 0.835, all P<0.05). Conclusion The levels of PCT, CRP and TNF-α in the patients with postoperative intestinal obstruction of colorectal cancer are higher than those of the control group.It can predict the occurrence of postoperative intestinal obstruction of colorectal cancer and can be used as a reference index for diagnosis and treatment of postoperative intestinal obstruction in colorectal cancer.

0.05). Expression of MDR1 had a positive ~correlation with mutant p53 accumulation and HER2 expression(P0.05 ).In univariate analyses,TNM staging, axillary lymph node metastasis, mutant p53 accumulation, and HER2 over-expression were negatively correlated with DFS and OS, and MDR1 over-expression significantly reduced OS but not DFS. In multivariate analysis, axillary lymph node metastasis, over-expression of MDR1 and HER2 were independent risk factors for prognosis. Conclusions ~Induction of multidrug resistance and poor response to chemotherapy and endocrinotherapy may be the chief reasons for poor prognosis of breast cancer with mutant p53 accumulation, and HER2 and MDR1 over-expression. ~Determination of the above genes′expression in breast cancer tissue can be of use in deciding the degree of ~malignancy , metastasis phenotype and prognosis of brest cancer. Increasing anthracycline dose may increase the ~overall response rate to chemotherapy and improve prognosis in patients with mutant p53 accumulation, HER2 and MDR1 over-expression, especially HER2 over-expression.

Objective: Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear. Methods: A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients' medical records. Multivariable logistic regression models were used to estimate the odds ratio ( Results: Overall, 158 (13.6%) patients were diagnosed with severe illness and 32 (2.7%) had unfavorable outcomes. Hypertension (2.87, 1.30-6.32), type 2 diabetes (T2DM) (3.57, 2.32-5.49), cardiovascular disease (CVD) (3.78, 1.81-7.89), fatty liver disease (7.53, 1.96-28.96), hyperlipidemia (2.15, 1.26-3.67), other lung diseases (6.00, 3.01-11.96), and electrolyte imbalance (10.40, 3.00-26.10) were independently linked to increased odds of being severely ill. T2DM (6.07, 2.89-12.75), CVD (8.47, 6.03-11.89), and electrolyte imbalance (19.44, 11.47-32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission (5.46, 3.25-9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes (6.58, 1.46-29.64) within two weeks. Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes.
Adult ; Aged ; COVID-19/virology* ; China/epidemiology* ; Comorbidity ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Severity of Illness Index ; Treatment Outcome