To evaluate the effectiveness of comprehensive psychological intervention for reducing the preoperative stress response in sterility women. Forty sterility women were equally divided into intervention group and control group. Heart rate(HR), blood pressure(BP), Zung anxiety grade and depression grade scores(SAS, SDS) were recorded; serum concentrations of glucose (GLU), cortieosteroid (Cor), adrenocorticotropic hormone(ACTH) and angiotensin (A Ⅱ) were measure in both groups at admission and before operation. These parameters before surgery were significant higher than those at admission for control group(P<0.05); but there were no significant differences for intervention group (P>0.05). The results indicate that comprehensive psychological intervention can reduce the preoperative stress response of sterility patients.