Aim of the study Aim of the study: The aim of our work intends to define of peripheral analgesic effect of the traditional drug Garidi 5 in experimental animal models. Objectives of the study: 1. To determinate the LD50 of traditional drug Garidi-5 2. To study of analgesic effect of Garidi-5 on experimental animal models Material and Methods: Mongolian traditional drug Garidi 5 was produced from the traditional drug manufacture of TMSTPCorporation of Mongolia. - The LD50 of Garidi-5 determined by Chan Chi (2005) - Analgesic effect was assessed by glacial acetic acid-induced writhing test (for peripheral action). All the animals used in the study were taken care of under ethical consideration, with approval from Ethical Committee Corporation and Minister of Health of Mongolia. Analgesia by peripheral action: The peripheral analgesic effect was tested by glacial acetic acid–induced writhing test in Albinomice (Wilkin et al,1961). Healthy mice of either sex weighing 20-30 g were fasted overnight and divided into three groups with six animals in each group. One hour after administration of drugs, induction of writhing was done in mice by giving intra peritoneal injection of acetic acid at a dose of 10 ml/kg body weight. The number of writhing responses were counted and recorded for 20 min. Traditional drug of Garidi 5 (20 mg/kg , 80 mg/kg, 200 mg/kg) was used as the test drug. Aspirin was taken as standard drug at a dose of 100 mg/kg p.o. (Ghosh, 2008) Results: Traditional drug Garidi-5 is LD50=2,28±0,15, it’s means that drug has low toxicity. Aqueous extract of Garidi 5 had significant peripheral analgesic effect as compared to control (p<0.05).

Background and Aim: The traditional herbal formulation of “Sendeng- 4”have been used to treat ulcers. The wound healing activity of traditional herbal formulations (Sendeng-4) containing Acacia catechu Willd (FamilyFabaceae), Terminalia chebula Retz, Terminalia bellarice (Family Combretaceae), Gardenia jasminoides (family Rubiaceae). We were prepared lotion of “Sendeng-4” was evaluated for its wound healing potential of incision wound models in mice’s. Material and Methods: Healthy inbred white mice’s were weighing 20-22 used for the study. The animals were randomly divided into two groups(n= 10) in all the models. The mice’swere anesthetized ketamine (12mg kg–1bodyweight) of the experimental wounds. We used comparable to that of lotion of thestandard drug and standard solution used for comparison in this present investigation. The results were also comparable of wound contracting ability, epithelization period, regeneration of tissues at the wound area. On Day 14-16th lotion of “Sendeng-4” treated animals exhibited 100% reductionin the wound area when compared to controls. The increased rate of woundcontraction, skin breaking strength and of “Sendeng-4” in wound healing. Conclution:The lotion of traditional herbal formulation of “Sendeng-4” have facilitateswound healing in the experimental animal model.

Objectives: To study of the effects on traditional drug Sendeng 4 for papain-induced osteoarthritis in rats. Methods: The rats were divided into three equal groups in number and were sacrificed under high-dose ether anesthesia after one, two, and four weeks of the last papain injection, respectively. Osteoarthritis was experimented in Wistar rats. Total 21 rats were injecting an admixture of 4% papain and its activator 0.03 M cycteine into the right knee joints on the first, fourth, and seventh days. The same volume of sterile saline solution was injected into the left knees as controls. Result. Pre sacrifice, measured with the artritis scoring evalution and X ray analysis, after cartilage from femorel condyles and tibial plateaus was macroscopically and histophatologically evaluated. The artritis scoring evalution were significantly lower at Sendeng 4 groups (2.2±0.16) compared to the control (3.7±0.25) groups. (p

Introduction: Diet therapy is based onbody constitution, disease conditions and seasonal influences in traditional medicine. Traditional principles of disease treatment and prevention with diet can be combined with conventional medical practices and may lead to a possibility of developing new treatment and preventive strategies. Objectives: To study the effects of interconnected factors of diet and season on some pathological changes of organ systems, we examined the effects of horse meat which has hot quality and goat meat which has cold quality on rat models of acute hepatitis induced by CCL4 and acute pulmonary edema induced by adrenalin hydrochloride in spring and autumn. Materials and methods: CCL4 (200 mg/kg) was was administered percutaneously to induce acute hepatitis in rats. Adrenalin hydrochloride (25 mg/kg) injected via the tail vein by slow injection to induce acute pulmonary edema in mice. Horse and goat meat were given for 14 days before the CCL4 and adrenalin hydrochloride administration. Renal activity was determined by measuring urine volume at every 6 hours. Results: Results of our study show that goat meat which has cold quality is more effective in autumn when a rise of bile element is occurred, and horse meat which has a cold quality is more effective in autumn when phlegm element rises. Horse meat increased pulmonary surfactant stabilitycoefficient by 54.73% in autumn while it increased that by 69.66% in spring. Decreased alveolar collapse and improved pulmonary surfactant stability by horse meat treatment support the concept of traditional medicine that diseases of the lungs, the phlegm dominant organs are treated with diet, medicine, and behavior with warm and oily qualities. In spring, urine excretion was increased in animalstreated with goat meat which has cold and oily qualities. Conclusions: 1. The “spring + horse meat” and “autumn + goat meat” versions of treatments more effectively decrease cytolysis during acute hepatocellular necrosis in animals administered CCL4. 2. Compare to goat meat, horse meat has more effects to decrease alveolar collapse and pulmonary edema in mice model of pulmonary edema induced by adrenalin hydrochloride. 3. “Goat meat + spring” version of treatment decreases urine excretion in experimental animals.