BACKGROUND: It can to a certain extent prevent complications during root canal preparation and improve the success rate of root canal therapy by understanding root canal anatomical configuration and bending degree.OBJECTIVE: To investigate the root canal curvature of permanent anterior teeth from Han Chinese people in Shandong province.METHODS: A total of 208 anterior teeth were collected and examined by indirect digital X-ray radiography both from labiolingual and mesiodistal directions,according to Schneider method,the data obtained were analyzed.RESULTS AND CONCLUSION: Most root canals of anterior teeth from Shandong province were typeⅠ,and more than half were curved. The most curvature happened in the apical third. The highest rate of canal curvature occurred in maxillary canines. Except the central incisors,the S-shaped canal was observed on the rest teeth. The radius of mandibular anteriors was obvious longer in labiolingual directions than that in mesiodistal directions. Root canal curvatures of anterior teeth from Shandong province are complex and have a higher bending rate.

Objective:To evaluate the effect of semiconductor lasers irradiation after routine root canal preparation on root cannal seal.Methods:60 Single-rooted freshly extracted human teeth were randomly divided into 6 groups(n=10).The crowns were removed at the cementoenamel junction and the roots were endodontically prepared with conventional methods.The roots in groups A and B were irradiated with 1 W semiconductor laser for 20 s,in group C and D were ultrasonically washed for 1 min,in group E and F without any treatment were used as the controls.Then all the roots were filled by vertical condensation of warm gutta-percha.The root cannal seal was evaluated with microleakage measurement.The data was analyzed by ANOVA.The teeth of group B,D and F were sectioned and examined under scanning electron microscope(SEM).Results:The microleakage(mm) of group A,C and E was 1.70±0.82,2.02±0.40 and 4.56±2.72 respectively(A vs E,P<0.01;C vs E,P<0.05;A vs C,P>0.05).SEM observation showed the melting,narrowness or closure of most dentinal tubules in group B,past and/or gutta-percha in the most dentinal tubules of group D.Conclusion:Semiconductor laser irradiation prior to root cannal filling can promote the effects of cannal seal.

Objective To investigate the clinical application value of genechip detection system in the mycobacterial species iden-tification and drug resistance analysis .Methods The specimens of sputum ,punctured pus ,pleural and abdominal ascites ,cerebro-spinal fluid and so on were performed the Mycobacterium DNA detection by using the gene chip technique .Then Mycobacterium tuberculosis positive samples were further performed the drug-resistant analysis .Meanwhile the Ziehl-Neelsen acid-fast staining was adopted to detect the sample .The positive rates were compared between the two groups .And TB-IGRA was used to examine the tubercle bacillus infection in partial patients .Results In 4402 samples ,137 cases (3 .36% ) of M ycobacterium tuberculosis (MTB) and 11 cases(7 .4% ) of non-tuberculosis mycobacterium(NTM) were detected .Puncture solution ,bronchoalveolar lavage fluid and tissue specimen had higher positive rate .In the 137 positive M TB samples by rifampicin-resistant gene rpoB ,isoniazide-re-sistant gene katG and inhA detection ,22 cases of resistance gene mutations were detected ;the positive rate of genechip for detecting sputum ,cerebrospinal fluid ,hydrothorax and ascites was higher than that of acid-fast staining .TB-IGRA detection had higher pos-itive rate of TB infection than genechip .Conclusion The genechip detection system can directly conduct Mycobacterium identifica-tion and drug resistance analysis ,which is especially suitable for sputum ,cerebrospinal fluid ,hydrothorax and ascites samples ,and which is simple and rapid with higher sensitivity and good specificity .

Gossypol was found to have an inhibiting effect on spermatogenesis in animals and has been used as an antifertility agent in clinical trial. However, a very small proportion of subjects on gossypol were found to be hypokaleimia. The direct cause of it was due to renal potassium loss. The present study was designed to make an ultrastructural observation on the enzymatic activity of the membrane protein Na-K-ATPase, the so called sodium pump of renal tubular cells of rats and guinea pigs following gossypol treatment. Gossypol was administered to male animals at a dosage of 30 mg/kg body weight daily. Kidney tissues were removed at various time intervals after gossypol treatment and processed for ultra-cytochemical study and quantitative measurement of Na-K-ATPase. The results obtained were as follows.1. The enzymatic activity of Na-K-ATPase of kidney tissues was shown to decrease slightly following gossypol treatment in both rats and giunea pigs, but there was no significant difference between the gos ol treated and the control groups.2. Ultra-cytochemical observations on the localization of Na-K-ATPase demonstrated that the enzyme reactive products in renal distal convoluting tubules, ascending limbs of Henle's loop and collecting ducts desending through the cortex to the outer medulla were mainly localized to the basal plasma membranesand their retromembranous folds of the epithelial cells. A small amount of the reactive products associated with the luminal surface of the distal convoluting and collective tubules were also observed. The amount of enzyme reactive granules of the treated animals did not differ significantly from that of controls.3. The results of perfusion experiments with ouabain indicated that ouabain inhibited Na-K-ATPase specifically in renal distal convolutions, ascending limbs and collecting tubules in both the treated and control animals.4. The cytochemical reactive granules in the erythrocyte and cardiac cell membrenes decreased in number following gossypol treatment.

Objective To explore the effect of antidepressant intervention on mood state of at or around the time of radiation therapy. Methods 62 hospitalized patients with cancer receiving radiotherapy were divided into two groups by the order of the time when they accept the radiotherapy which called intervention group ( antidepressant fluoxetine group) and control group. And the two groups patients filled out Hamilton Anxiety Scale( HAMA )and Hamilton Depression Scale (HAMD) at or around the time of radiation therapy to investigate the effects of fluoxetine on anxiety and depression. Results The scores of HAMA and HAMD showed that there was no differences between the intervention group and the control group before the radiotherapy. There was significant difference during the radiotherapy ( 6.41 ± 2.30 ) vs ( 9.29 ± 5.62 ); ( 8.80 ± 3. 048 ) vs ( 12.22 ± 8.32 ) and two weeks after the end of the radiotherapy ( 4.90 ± 1.71 ) vs ( 12.32 ± 7.24 ); ( 6.83 ± 2.47 ) vs ( 16.09 ± 8.61 ) between antidepressant fluoxetine group and control group(P ＜ 0.05 ). Conclusion More attention should be paid to mental health and antidepressant can alleviate anxiety and depression of the cancer patients'at or around the time of radiotherapy.

Objective To establish a simple model for arteriosclerosis(AS)screening to provide a viable tool for the timely identification of AS risk among residents aged 40～65 years.Methods Data were obtained from the Sleep and Chronic Diseases Program in Fuquan City.The original dataset was divided into a training subset and a validation subset(80%:20%).LASSO and logistic regression models were used to screen variables,perform multivariate regression analyses.Internal validation was performed using the Bootstrap method.Nomogram Plot was constructed,and risk score thresholds were determined based on ROC curves to classify high-risk populations.Results RS Model was established to include age,gender,napping,sleep efficiency,sleep disorders,hyperten-sion and diabetes,with AUC=74.80%and a model risk score threshold=84.20.PHC Model was established to include age,gender,napping,sleep efficiency,systolic blood pressure,fasting blood glucose,and pulse variables,with AUC=82.80%and a risk score threshold of 78.00.Decision curves showed that both models performed well in terms of calibration and actual benefits for health management.Conclusion The two AS screening models exhibit acceptable accuracy and differentiation.Therefore,it can be applied in residents'self-health management and in primary care organizations'screening work in a large scale.

Objective:To analyze the association between body health score and the risk of hypertension among health examination population aged 40-65 years.Methods:This study was a cross-sectional study, and 1 104 people aged 40-65 years who underwent physical examination at the Physical Examination Centre of the First People′s Hospital of Fuquan City from March to November 2022 were selected. Clinical data, such as general information, physical examination, body composition and history of hypertension diseases, were collected. The body health score was reported by the Xiaomi Body Fat Scale′s accompanying exercise health software, and was calculated by combining body fat, water and other body composition data. The association between body health score and the risk of hypertension was analyzed using restricted cubic spline regression models, while a sensitivity analysis and sex-stratified analyses were performed. Multivariate logistic regression combined with stratified analysis was used to explore the association between dimensions of body composition and the risk of hypertension.Results:The body health score was significantly lower in hypertensive patients than in non-hypertensive patients among the 1 104 health examination population [52.0(30.0) vs 69.0(35.8) points] ( Z=-8.547, P<0.001). The lower the body health score, the higher the risk of hypertension ( χ2=18.48, PNonlinear<0.001). In the total population, high body mass index was associated with an increased risk of hypertension ( OR=1.744, 95% CI: 1.104-2.765), high protein content was associated with a reduced risk of hypertension ( OR=0.587, 95% CI: 0.344-0.982) (both P<0.05). Gender-stratified analyses showed that high protein content was associated with a reduced risk of hypertension only in men ( OR=0.233, 95% CI: 0.080-0.592) ( P=0.004). High body mass index was positively associated with the risk of hypertension when the body health score was ≥60 points ( OR=2.378, 95% CI: 1.255-4.542) ( P=0.008). High visceral adiposity index (VAI) was positively associated with the risk of hypertension when the body health score was <60 points ( OR=4.395, 95% CI: 1.466-13.620), and high protein content was negatively associated with the risk of hypertension ( OR=0.255, 95% CI: 0.091-0.638) (all P<0.05). Conclusions:Health examination population aged 40-65 years with lower scores of physical health are more likely to have a risk of hypertension. Men should pay attention to the impact of body protein in hypertension risk prevention and control. The effect of body mass index should be noted when body health scores are ≥60 points, and the effect of VAI and body protein should be considered when body health scores are <60 points.