Objective To clarify the role of cardiomyocyte apoptosis in cardiac function after transplantation of bone marrow cells. Methods A mycardial infarction model was induced in SD rats by left anterior descending artery ligation. 5?106 of bone marrow mononuclear cells were injected into peri-infarct zone (BMT group). In 4 or 8 weeks post-infarct, apoptosis of cardiomyocytes and expression of Bcl-2/Bax protein were determined by TUNEL or immunohistochemistry. Cardiac function was evaluated by echocardiography (UCG). Results TUNEL results indicated that apoptosis index of peri-infarct zone from the BMT group was reduced significantly when compared with the control group (4 wk: 0.094?6?0.017 vs 0.173?0.018, P

Objective To characterize the effects of TPP1 knockdown on Pot1a and Pot1b localization at telomeres and on the telomere end protection.Methods Knockdown of endogenous TPP1 in mouse embryonic fibroblasts(MEFs) with the retrovirus vector encoding shRNA targeting TPP1,IF/PNA-FISH was performed to determine the localization of Pot1a and Pot1b at telomeres,and TdT-FITC was applied to characterize the effects on the function of telomere end protection,cellular senescence was analyzed by SA-beta gal staining,and phosphorylated p53ser18 and p21 were examined by Western blotting.Results Pot1a and Pot1b were unable to localize at telomeres in about 65% of MEFs with TPP1 knockdown,while that was found in less than 5% of MEFs without TPP1 knockdown(t=10.96,P

This paper presents a method for estimating three-dimensional (3D) motion of coronary arteries from single-plane X-ray angiogram sequences on two views. Firstly, original images are preprocessed and two-dimensional (2D) vessel skeletons are extracted. 2D motion estimation is performed along the skeletons in two images. Then geometrical transformation matrix between views is obtained based on perspective projection model for X-ray angiography system, and 3D coordinate of spatial points are calculated. The 3D motion estimation and reconstruction algorithm is applied along the two image sequences to accomplish 3D reconstruction of vessel skeletons and motion vectors between consecutive time instants. Its effectiveness has been demonstrated on clinical single-plane coronary artery angiograms and potential errors are discussed.
Algorithms ; Coronary Angiography ; methods ; Coronary Disease ; diagnostic imaging ; Electrocardiography ; Humans ; Imaging, Three-Dimensional ; methods ; Movement ; Radiographic Image Interpretation, Computer-Assisted