The adolescence group is a unique group that deserves being paid close attention to.In terms of public crisis events,they are lack of essential sense of crisis and proper evaluation of crisis information,thus unable to protect and adjust their mental status.Correct responses to public crisis events for adolescence include a re-recognition of the real world,a positive adjustment of mental emotions,effective solutions to problems,and exploration for social support.In terms of intervention of adolescence mental crisis,it is important to implement early stage evaluation and intensification therapy,crisis counseling and treatment,crisis evaluation and intervention,and the usage of crisis intervention hotline.

Objective To evaluate the relationship between peritoneal transport type and serum C-reactive protein (CRP) in maintenance peritoneal dialysis (MPD) patients.Methods Standard peritoneal equilibration test (PET) was performed in 56 MPD patients (MPD group) with regular follow-up.According to D/P values,56 patients were divided into high permeability group (D/P ＞ 0.65,18 cases) and low permeability group (D/P ≤ 0.65,38 cases).In parallel at the date of PET examination,serum creatinine,blood urea nitrogen,uric acid,CRP and dialysate creatinine,blood urea nitrogen,uric acid was tested with an automatic biochemical analyzer,and urea clearance index and creatinine clearance rate was calculated.Dialysis prescription was formulated according to PET results to reach the criteria urea clearance index ≥ 1.7 and creatinine clearance rate ≥ 50 L/(week· 1.73 m2).Six months after MPD treatment,these indexes were detected again.And 20 cases of healthy person (control group) and 30 cases of uremic non-dialysis patients (uremic non-dialysis group) were selected randomly.Results The serum CRP level in high permeability group,low permeability group,uremic non-dialysis group was higher than that in control group [(54.41 ± 17.77),(43.34 ± 18.07),(39.10 ± 17.86) mg/L vs.(2.00 ±0.36) mg/L,P＜ 0.05].The serum CRP level in high permeability group was higher than that in low permeability group (P ＜ 0.05).There was no significant difference in the serum CRP level between MPD group and uremic non-dialysis group (P ＞0.05).There was no significant difference in the serum CRP level between 6 months after MPD and the date of the PET examination in high permeability group,low permeability group (P ＞ 0.05).Six patients with low permeability peritoneal transport changed into the high permeability at 6 months after MPD.The serum CRP level in these 6 patients at 1 month after catheter were significantly higher than the other patients of the low permeability [(64.45 ± 13.05) mg/L vs.(39.38 ± 16.12) mg/L,P ＜ 0.05].Conclusions Uremic patients in vivo exist micro-inflammatory state.The peritoneal transport characteristics of MPD patients are mainly in low permeability.Peritoneal transport characteristics of high permeability in vivo in patients with existing micro-inflammatory status are more severe than those in patients with low permeability.MPD treatment can not change the serum CRP levels in uremic patients.The original micro-inflammatory state in uremic patients may affect their peritoneal transport type.

Although methyltransferase has been recognized as a major element that governs the epigenetic regulation of the genome during temozolomide (TMZ) chemotherapy in glioblastoma multiforme (GBM) patients, its regulatory effect on glioblastoma chemoresistance has not been well defined. This study investigated whether DNA methyltransferase (DNMT) expression was associated with TMZ sensitivity in glioma cells and elucidated the underlying mechanism. DNMT expression was analyzed by western blotting. miR-20a promoter methylation was evaluated by methylation-specific PCR. Cell viability and apoptosis were assessed using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and TdT-mediated dUTP-biotin nick end labeling assays, respectively. The results showed that compared with parental U251 cells, DNMT1 expression was downregulated, miR-20a promoter methylation was attenuated and miR-20a levels were elevated in TMZ-resistant U251 cells. Methyltransferase inhibition by 5-aza-2\'-deoxycytidine treatment reduced TMZ sensitivity in U251 cells. In U251/TM cells, DNMT1 expression was negatively correlated with miR-20a expression and positively correlated with TMZ sensitivity and leucine-rich repeats and immunoglobulin-like domains 1 expression; these effects were reversed by changes in miR-20a expression. DNMT1 overexpression induced an increase in U251/TM cell apoptosis that was inhibited by the miR-20a mimic, whereas DNMT1 silencing attenuated U251/TM cell apoptosis in a manner that was abrogated by miR-20a inhibitor treatment. Tumor growth of the U251/TM xenograft was inhibited by pcDNA-DNMT1 pretreatment and boosted by DNMT1-small hairpin RNA pretreatment. In summary, DNMT1 mediated chemosensitivity by reducing methylation of the microRNA-20a promoter in glioma cells.
Animals ; Antineoplastic Agents, Alkylating/*pharmacology/therapeutic use ; Apoptosis/drug effects ; Brain/drug effects/metabolism/pathology ; Brain Neoplasms/drug therapy/*genetics/pathology ; DNA (Cytosine-5-)-Methyltransferase/antagonists & inhibitors/*genetics/metabolism ; DNA Methylation ; Dacarbazine/*analogs & derivatives/pharmacology/therapeutic use ; Drug Resistance, Neoplasm ; Female ; Gene Expression Regulation, Neoplastic ; Glioma/drug therapy/*genetics/pathology ; Humans ; Mice, Inbred C57BL ; MicroRNAs/*genetics ; Promoter Regions, Genetic

Drinking culture has high significance in both China and the world, whether in the entertainment sector or in social occasions; according to the World Health Organization's 2018 Global Alcohol and Health Report, about 3 million people died from excessive drinking in 2016, accounting for 5.3% of the total global deaths that year. Oxidative stress and inflammation are the most common pathological phenomena caused by alcohol abuse (Snyder et al., 2017). Scutellarin, a kind of flavonoid, is one of the main active ingredients extracted from breviscapine. It exerts anti-inflammatory, antioxidant, and vasodilation effects, and has been used to treat cardiovascular diseases and alcoholic liver injury. Although scutellarin can effectively alleviate multi-target organ injury induced by different forms of stimulation, its protective effect on alcoholic brain injury has not been well-defined. Therefore, the present study established an acute alcohol mice brain injury model to explore the effect of scutellarin on acute alcoholic brain injury. The study was carried out based on the targets of oxidative stress and inflammation, which is of great significance for the targeted therapy of clinical alcohol diseases.
Animals ; Apigenin/therapeutic use* ; Brain Injuries/drug therapy* ; Glucuronates/therapeutic use* ; Humans ; Mice ; Oxidative Stress

随着铜绿假单胞菌（铜绿）的耐药性逐年增强，铜绿感染已经成为公共医疗卫生的重点关注问题。线粒体自噬及其介导的线粒体功能障碍在多种细菌感染中已被报道，但线粒体功能障碍在宿主调控铜绿感染中的作用尚不明确。因此，本研究建立铜绿刺激小鼠巨噬细胞感染模型和小鼠急性铜绿感染模型，探讨铜绿是否通过诱导线粒体自噬改变线粒体功能，进而影响宿主免疫炎症反应和细胞毒性，并通过监测生存率和肺组织病理学变化进一步确定线粒体自噬在小鼠铜绿体内感染模型中的作用。结果表明，铜绿引起小鼠腹腔巨噬细胞线粒体功能障碍，并通过线粒体自噬途径清除铜绿刺激引起的活性氧（ROS）累积，从而抑制铜绿引起的促炎性细胞因子分泌并增强细胞毒性。体内实验进一步确认线粒体自噬在铜绿体内感染中的作用。
Mice ; Animals ; Reactive Oxygen Species/metabolism* ; Pseudomonas aeruginosa ; Macrophages/metabolism* ; Mitochondria ; Cytokines/metabolism*