Objective: To study the effects of compress lung drain fluid(CLDF) and sustained inflation( SI) on beagle dogs with moderately and severe acute respiratory distress syndrome. Methods: twelve beagle dogs were randomly assigned to two groups. SI was applied at pressure of 35 cmH2O -40 cmH2O for 20s in one group, compress lung drain fluid (CLDF) was applied at pressure of 15cmH2O- 20 cmH2O for 15 s in anther group, then was continued ventilation, and blood gas ,and lung mechanics were observed. Results; Pressure of arterial oxygen and arterial saturation were improved in CLDF group( P 0. 05 ). PIP ,Pm ,Pp were decreased in CLDF group( P

Objective To explore the application value of ATP bioluminescence in assaying the cleaning quality of uterine suction.Methods According to the principle of random,we selected 120 uterine suction tube after use.60 used tubes in A group were given water immersion + high-pressure water jets wash + dry method,60 used tubes in B group were given alkaline cleaning solution soak + ultrasonic + high-pressure water washing + manual scrub + drying method.The quality of suction tube was initially tested with the visual method after cleaning,Straw inside the top cavity,2.5cm at inside straw joint and the front of straw outer wall,10cm at suction cavity wall,before and after cleaning the residues of bacteria were assayed with the ATP bioluminescence and bacteria counting method for parallel comparison.Results After cleaning drying program,the RLU of two groups in suction head internal top,2.5cm at inside straw joint,the front of straw outer wall,10cm at suction cavity wall had significant differences (t =26.81,29.13,7.14,55.74,P ＜ 0.01).The cleaning effect of B group was better than A group..Bacteria counting method was used to detect pathogenic microorganism that was not detected.The qualified rate of cleaning at four parts was as following:group A:70.0％,85.0％,93.3％,93.3％.B group:95.0％,98.3％,100.0％,100.0％,and the qualified rate of group B was significantly higher than group A (x2 =12.99,6.98,4.14,4.14,all P ＜ 0.05).Conclusion ATP bioluminescence method can dynamically rapid detect the bacteria before and after intrauterine suction cleaning residues,can be viewed as an effective method to detect quality of uterine suction cleaning.

Objective To observe the effects of IL-4 on the expression of epithelial sodium channel(ENaC) ?-subunit.Methods A549 cells were cultured with IL-4 at the concentration of 0,0.1,1 and 10 ng/ml for 12 h or with 1 ng/ml IL-4 for 3,6,12,24 and 48 h.Then ?-ENaC protein and mRNA expressions were detected by Western blotting and RT-PCR respectively.Results IL-4 decreased the expression of ?-ENaC in A549 cells in a time-and dose-dependent manner,via gene transformation.When A549 cells were cultured with IL-4 at the concentration of 1 ng/ml for 3,6,12,24 and 48 h,the expression of ?-ENaC protein and mRNA were decreased in a time-dependent manner.Statistical analysis showed culture for 6 h had a significant difference compared with other time points(P

Objective To investigate the effects of adenosine receptor A2a on the expression of epithelial sodium channel ?-subunit (?-ENaC) in alveolar epithelium A549 cells and the effects of adenosine receptor A2a in acute lung injury/aute respiratory distress syndrome. Methods After alveolar epithelium A549 Cells were incubated with 0,0.1,1,10 and 100 ?mol/L adenosine receptor A2a agonist CGS-21680 for 8 h or with 100 ?mol/L CGS-21680 for 0,1,4,8,24 and 48 h respectively,the mRNA and protein levels of ?-ENaC were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis respectively. Results After A549 cells were incubated with CGS-21680 at different doses for 8 h,the mRNA and protein levels of ?-ENaC were elevated significantly at 0.1 ?mol/L CGS-21680 treatment compared with the control group (P

0.05).The ENaC-? mRNA and protein expression level in A549 cells in 6,12 and 24 h after treatment with ulinastatin (at the concentration of 5 000 U/ml) + TNF-? was significantly increased compared with that after treatment with TNF-? (P

Objective To study the expression of lectin-like oxidized LDL receptor-1 (LOX-1) protein in acute lung injury (ALI) and the effect of captopril on this expression in order to investigate its role in the process and preliminary intervention.Methods Sprague-Dawley rats were randomly divided into 3 groups,normal control group,lipopolysaccharide (LPS) groups,and a LPS+captopril group,each group 10 rats.ALI model was induced by intratracheal injection of LPS (5 mg/kg),then those of LPS+captopril group were give an intraperitoneal injection of captopril (1.25 mg/kg).The animals of normal control group received an intratracheal injection of normal saline.In 6 h after LPS injection,the level of p(O2),wet/dry ratio (W/D),concentration of total protein in bronchoalveolar lavage fluid (BALF) and lung tissue histopathological changes were examined.The expression of LOX-1 protein in the lung tissue was measured by Western blot analysis.HE staining was used to examine the pathological changes of the lung.Results Histological examination showed that extensive lung inflammation were seen in the LPS group,which manifested by accumulation of significant numbers of neutrophils.The level of p(O2) in LPS group [(6.86?0.75) kPa] was decreased compared with that in sham group [(12.14?0.60) kPa,P

[ ABSTRACT] AIM:To investigate the effect of adipolin/CTRP12 in LPS-induced acute respiratory distress syn-drome (ARDS) and its potential regulation on alveolar epithelial sodium channel (ENaC) in mice.METHODS:C57BL/6J mice (n=40) were randomly divided into control group, LPS group, adipolin group and wortmannin (PI3K inhibitor) group with 10 mice in each group using random number table.The pathological changes of the lung tissues were evaluated by HE staining.The alveolar fluid clearance ( AFC) was measured by Evans blue-marked albumin, and the concentrations of total protein in bronchoalveolar lavage fluid ( BALF) were assessed by bicinchoninic acid ( BCA) method.In BALF, the levels of IL-1βand TNF-αwere determined by ELISA, and the activity of myeloperoxidase ( MPO) was detected by an MPO assay kit.The total cell counts and polymorphonuclear neutrophil ( PMN) counts in the BALF were analyzed by Gi-emsa staining.The mRNA levels of α-ENaC were assessed by qPCR, while the protein levels of α-ENaC and p-Akt were determined by Western blot.RESULTS: Compared with control group, the classic ARDS pathological changes were ob-served in the mice in LPS group, manifesting by severe pathological lung injury (P<0.05), increases in W/D weight rati-o, total protein levels, cell counts, MPO activitiy, and IL-1βand TNF-αlevels in the BALF, and decrease in AFC ( P<
0.05), accompanied by down-regulated levels of α-ENaC and p-Akt in the lung tissues (P<0.05).The deteriorating effects triggered by LPS were significantly reversed by administration of adipolin.However, PI3K inhibitor wortmannin can-celed the beneficial effects of adipolin on LPS-induced ARDS, as evidenced by aggravated lung injury, increased levels of W/D weight ratio, protein levels, cell counts, MPO activity, and IL-1βand TNF-αlevels in the BALF (P<0.05), and decreased levels of AFC,α-ENaC and p-Akt in the lung tissues.CONCLUSION:Adipolin protects against LPS-induced ARDS in the mice by up-regulatingα-ENaC and enhancing AFC via PI3K/Akt signal pathway.

Objective To construct recombinant lentivirus silence vector aiming at rictor gene in mTORC2 specific protein, and to investigate its regulation on mTORC2/SGK1 signal pathway and the effect on pulmonary alveolar epithelial sodium ion chan-nel,as well as the role in acute respiratory distress syndrome(ARDS)and acute lung injury.Methods The interfering vector plas-mid and empty vector plasmid of target gene rictor were constructed,which and the lentivirus packaging system were co-transfected to 293T cells.The viral supernatant was collected,centrifuged,concentrated and purified for obtaining recombinant lentivirus.The virus titer was detected and the virus was infected to A549 cells.Stable cell lines were screened.RT-PCR was used to confirm the silencing situation of target gene rictor.The expression situation of various signal indexes in this pathway was detected by PCR and Western blot.Results The recombinant lentivirus of silence gene rictor was successfully constructed and transfected to A549 cell for obtaining stable cell lines.Compared with blank and control groups,the mRNA levels of rictor,downstream SGK1 andα-,β-andγ-ENaC in the shRNA-rictor group were significantly decreased (P <0.05 ).Meanwhile,the protein levels of rictor,downstream SGK1,P-SGK andα-,β-andγ-ENaC in the shRNA-rictor group were significantly decreased compared with the other two groups(P<0.05).Conclusion Silence rictor gene has the obvious regulation effect on mTORC2/SGK1 signal pathway,meanwhile affects the expression of pulmonary alveolar epithelial cellular α-,β-and γ-ENaC at gene and protein level.It is speculated that mTORC2/SGK1 may be an important signal pathway for regulating the clearance capacity of pulmonary alveolar epithelial cells on pulmonary alveolar fluid and simultaneously affecting the pulmonary edema formation.

Objective To explore the peroxisome proliferator activated receptor gamma (PPARγ) expression characteristic of lymphocytes in patients with asthma .Methods Collected blood samples from healthy subjects (health control group) and asthmatic patients(asthmatic group) before treatment ,2 and 4 days after treatment .Expression levels of PPARγtested with Q-PCR .Analyzed eosinophil percentage of induced sputum ,IL-5 concentration in sputum supernatant measured with ELISA kits .Results Compared with healthy control group ,the eosinophil percentage and IL-5 concentration were higher in asthmatic group before treatment ;meanwhile the expression level of PPARγwas at the lowest .After treatment ,PPARγgradually increased accompanied with eosino-phil percentage and IL-5 concentration gradually decreased .Conclusion Asthmatic patients had a lowest PPARγ expression level . Their recovery perhaps attributed to the up-regulation of PPARγin lymphocytes .The anti-inflammatory effect of PPARγachieved might be via inhibiting the function of Th2 cells .

Objective To investigate the effect of resveratrol on alveolar epithelial sodium channel in acute lung injury mice and the potential mechanism.Methods Twenty-four C57BL/6 mice were randomly divided into control group, LPS group, RES group and PP242(mTORC inhibitor) group with 6 mice in each group.The pathological changes in lung tissue were evaluated by HE staining;the concentrations of total protein in bronchoalveolar lavage fluid (BALF) were assessed by BCA (bicinchoninic acid).The levels of inflammatory cytokines in BALF were determined by ELISA.The proportions of polymorphonuclear neutrophil (PMN) in BALF were detected by Flow Cytometry.The transcription levels of α-ENaC mRNA were assessed by qPCR while the protein levels of α-ENaC and p-GSK1 were measured by Western blot.Results 1)Compared with mice in control group, severe pathological lung injury changes were observed in mice of LPS group, with increased total protein levels, PMN proportions,levels of inflammatory cytokines in BALF (P<0.05), accompanied by down-regulated level of α-ENaC and p-SGK1 in lung tissues (P<0.05).2)Compared with mice in LPS group, resveratrol significantly reversed lung injury triggered by LPS, decreased total protein levels, PMN proportions, levels of inflammatory cytokines in BALF (P<0.05), with down-regulated levels of α-ENaC and p-SGK1 in lung tissues (P<0.05).3)However, PP242 prevented beneficial effects of RES on ALI.Conclusions Up-regulation of α-ENaC expression via activation of SGK1 takes part in the protective effects of RES on LPS-induced ALI in mice.