BACKGROUND:Implant related problems such as loosening,dislocation and infection often come along with joint replacement.Nanotechnology provides a new insight into the preparation of joint replacement implants.OBJECTIVE:To elaborate the research progress of nanotechnology in improving joint replacement implants.METHODS:The first author used the computer to retrieve PubMed databases using the key words of joint replacement,artificial implant,nanotechnology,nano-materials,nanoscales,biocompatibility in English,to find relevant literature on nanotechnology and joint replacement implants.All data were primarily screened to exclude repeated and irrelevant articles,and finally 51 articles related to the study were retained.RESULTS AND CONCLUSION:Through reviewing these 51 articles,we make a detailed introduction about some of the biological responses that occur with nano-materials,and some of the biocompatibility problems that have been raised in relation to materials,as well as the ways that have been employed to improve biocompatibility of nano structured materials.But the long-term effect of nanotechnology on the human body is still worthy of further research in medicine or related fields.

Objective To investigate the effects of calcium pyruvate supplementation on body weight gain and fatty acid metabolism in rats.Method Thirty-two 150 g male SD rats were randomly divided into four groups:control group,low,middle and high dose calcium pyruvate group (0,100,500 and 1000mg/(kg.d)).Calcium pyruvate was administered intragastrically to rats-for 4 w,and then the rats were killed and blood and tissue samples were collected.Serum lipids and blood glucose were determined by biochemistry analyzer.Analysis of fatty acid composition of adipose tissue,liver tissue and musculature was determined by capillary gas chromatography and mRNA expressions of ACC-? in liver by RT-PCR.Result At the end of the experiment,the body weight and food conversion efficiency of rats in all experiment groups were significantly lower than that of control group.TG of high and middle dose group were obviously lower than that of control group.TC and blood glucose concentration were not statistically different among different groups.contents of C16:0,c9-C18:1,c9,c12-C18:2,?-C18:3 and C20:4 in the adipose tissue were significantly decreased in the experiment groups,but the content of C18:0 was not statistically different on fatty acid composition of musculature in rats.Fatty acid composition of liver tissue was remarkably lower than that of the control group,except C18:0 and c12-C18:2.mRNA expression level of ACC in liver of high dose group was significantly decreased (P

Objective To introduce the construction and application of clinical microbiology laboratory data management expert system.Methods Firstly, the process management was introduced to clinical microbiology laboratory. Then the characteristics of data on each node of work process were analyzed, and SQL Server data table was created as the knowledge base of the expert system.Finally, VB6.0 was used to compile the knowledge acquisition module, reasoning desktop module and input/output interface procedures to finally construct the expert system.Rates of defect report, errors in bacterial identification and drug sensitivity test, delay in culture results reporting and average delayed days were compared before and after the application of the expert system.Results The expert system could be used for data management in process nodes like sample reception, information collection and input, bacteria culture medium selection, bacterial identification and drug sensitive test, interpretation of drug sensitivity results, comprehensive evaluation in bacterial identification and drug sensitivity results, report of negative result, report of positive result, blood culture, Mycoplasma culture, time limit of detection, and nosocomial infection indicators.No defect report was found after the application of expert system; rate of errors in selection of drug sensitivity test medium was reduced from 0.81% ( 31/3 836 ) in 2012 to 0.02%(1/5 433) in 2014;rate of delay in culture results reporting was reduced from 1.78% (320/17 983) to 1.18%(232/19 692), and the average delayed days was also reduced (3.8 d vs.3.2 d).Conclusion Clinical microbiology laboratory data management expert system can improve work efficiency and reduce errors, which can enhance the overall management of laboratory and the quality of clinical service.

Relying on the practice platform of P2 laboratory, researchers reform and explore the contents and forms of experimental teaching for pathogenic biology and immunology, creating an innovative experimental teaching system suitable for training medical students' professional quality and innovative ability. By improving teaching conditions (construction of P2 laboratory), improving teaching methods and innovating experimental teaching contents, and introducing comprehensive design experiment, students' consciousnesses of service and biosafety have been strengthened, meanwhile, their ability to analyze and solve problems has been improved, and their critical and creative thinking has been cultivated.

Therapeutic HIV vaccine was considered as a hopeful curative method for AIDS patients. However, there is still no suitable HIV animal model for vaccine study since the difference in the immune system between human and animals. To evaluate the therapeutic effect of combined immunization strategy with multiple vector vaccines in macaque models. Plasmid DNA, recombinant Ad5 and MVA vaccines which expressing SIV gag and env genes were constructed. Sequential and repeated immune strategy were applied to immunize mice with these three vaccines. Cellular immune responses in mice immunized with these three vaccines were measured by ELISPOT test in vitro and CTL assay in vivo. The results were analyzed and compared with different antigen combination, order of vaccines and intervals to choose a suitable immunization strategy for macaque immunization in future. It indicated that strong SIV-Gag/Env-specific cellular immune responses were induced by these three vector vaccines. It laid a foundation for evaluating the therapeutic effect of combined immunization strategy with multiple vector vaccines in SIV infected macaque models.
AIDS Vaccines ; administration & dosage ; genetics ; immunology ; Adenoviridae ; genetics ; metabolism ; Animals ; Antibodies, Viral ; immunology ; Female ; Gene Products, env ; administration & dosage ; genetics ; immunology ; Gene Products, gag ; administration & dosage ; genetics ; immunology ; Genetic Vectors ; genetics ; metabolism ; HIV Infections ; immunology ; prevention & control ; virology ; Humans ; Immunization ; Mice ; Mice, Inbred BALB C ; Simian Immunodeficiency Virus ; genetics ; immunology ; Vaccines, DNA ; administration & dosage ; genetics ; immunology

Hand ; Humans ; Occupational Exposure ; Vibration ; Workplace

OBJECTIVE: To analyze the changes of finger skin temperature in cold provocation test( CPT) in workers with vibration white finger( VWF). METHODS: A total of 245 male workers engaged in hand arm vibration operation was selected as study subjects using random number table method. All subjects were divided into VWF group( 73 persons) and control group( 172 persons). CPT( 10 ℃,10 min) was performed and the skin temperature of 6 fingers( index finger,middle finger and ring finger of both hands) was measured at pre-CPT adaptation period( 0,10,20,30 min) and after CPT period( 0,5,10,15,20,25,30 min). RESULTS: The effect of interaction between grouping and observe time was statistically significant on finger skin temperature( P < 0. 01). In the pre-CPT adaptation period,there was no statistically significant difference on skin temperature between 10 and 30 min time point in the two groups( P > 0. 05). After CPT,the fingers skin temperature of VWF group was lower than that of control group at 5 min time point( P < 0. 05),but there were no statistically significant differences on fingers skin temperature of other time points between the two groups( P > 0. 05).In both groups,the finger skin temperature at 0 min time point after CPT were lower than other time points in the same group( P < 0. 05),and the finger skin temperature increased with time( P < 0. 01). However,the finger skin temperature at 30 min after CPT did not restore to that at 30 min time point of pre-CPT. Except the VWF group,the abnormal rewarming temperature at 5 min time point after CPT of left index finger,the right index finger and the right ring finger were higher than that of the control group( 72. 6% vs 56. 4%,75. 3% vs 57. 6%,86. 3% vs 65. 1%,P < 0. 05),but there were no statistically significant differences on the abnormal rewarming temperature at 10,30 min time points of the six fingers in the two groups( P > 0. 05). There were no statistically significant differences on the detection rate of abnormal rewarming temperature between left index finger and the right index finger,or the right index finger and the right ring finger in the VWF group( 72. 6% vs 75. 3%,75. 3% vs 86. 3%,P > 0. 05). CONCLUSION: When CPT( 10 ℃,10 min) was performed in workers engaged in hand arm vibration operation,it is recommended to measure the finger skin temperature of index finger,and adaptation time before CPT can be adjusted to 10 min.

Smart dressings, which show obvious advantage and potential in wound treatment and real-time monitoring, attract widespread attention in recent years. Real-time and dynamic acquiring wound information is vital to the treatment and prognosis of wound. Further research on smart dressings is helpful for wound management, personalized treatment, and realization of medical application translation of health monitoring technology. In the article, we categorize smart dressings and conclude their functions according to the type of micro-environment information of wound gathered by smart dressings.

Objective To investigate the clinical isolation situation and drug resistance features of enterococcal bacteria in order to provide reference for the clinical rational use of antibacterial agents and infection control. Methods A total of 1220 strains of enterococcal bacteria that induced hospital infection were analyzed retrospectively. Walk Away 96 automated microbial analyzer was used for strain identification and drug sensitive test. MIC was used for screening high-level aminoglycoside resistant strains. WHONET 5.6 was used for data analysis. Results A total of 1220 strains of enterococci were detected, including 675 strains of enterococci faecalis, accounting for 55.3%, and 445 strains of ente-rococci faecium, accounting for 36.5%. Enterococcal bacteria mainly distributed in clinical urine specimens, accounting for 57.5%. The total drug resistance rate of enterococci faecalis was high and the drug resistance rates to penicillin, ampicillin, ciprofloxacin and levofloxacin were all higher than 90%, which were significantly higher than those of the enterococci faecium (<17%). The drug resistance rate of enterococci faecalis to quinupristin/dalfopristin was 100.0%and that of enterococci faecium was 12.6%. For both types of bacteria, no strain resistant to vancomycin was found, but 3 strains of enterococci faecalis were resistant to linezolid. The screening rates of enterococci faecalis for high-level gentamicin drug resistant strains and high-level streptomycin resistant strains were 54.1% and 27.3% respectively while those of enterococci faecium were 58.2% and 56.9% respectively. Conclusion The drug resistance situation of enterococcal bacteria to common antibacterial drugs is not optimistic, and the monitoring of clinical distribution and drug resistance situation of enterococcal bacteria is of important guiding significance to the clinical treatment of entero-coccal bacterial infection.

Objective To investigate the acute phase expression of serum cytokines in children with Kawasaki disease (KD) and its association with coronary artery lesions (CALs). Methods Expression of 13 cytokines in serum of 104 KD patients including 11 cases with CALs and 74 febrile control cases admitted to the hospital from October 2016 to March 2017 were retrospectively analyzed. Results Interleukin (IL)-8, IL-1, IL-2, IL-6, IL-10, IL-17A, IL-18, tumor necrotic factor-α and SCD25 (IL-2R) were declined significantly after intravenous immunoglobulin administration (IVIG) in KD patients (all P<0.05). Compared with the fever control group, Pre-IVIG, an IL-17A level ≥ 0.155 pg/mL had a sensitivity of 50% and 93.2% specificity for predicting KD; IL-18 level ≤ 15.43 pg/mL had a sensitivity of 71.2%, and the specificity was 54.1%; SCD25 (IL-2R) ≥ 29475.29 pg/mL had a 65.4% sensitivity and 81.1% specificity for predicting KD. Before IVIG treatment, the level of IL-10 was significantly lower in KD patients with CALs than in those without CALs. Conclusions Determination of various cytokines profile may be helpful for predicting the disease prognosis and targeting treatment strategies in patients with KD.