Objective To construct the risk model for healthcare-associated infection (HAI)with multidrug-re-sistant organisms(MDROs)in intensive care unit (ICU).Methods 836 patients who were admitted to ICU for more than 48 hours between October 2012 and September 2015 were analyzed retrospectively,logistic regression model of HAI was constructed,the model was conducted goodness of fit tests and the area under ROC curve analysis. Results Among 836 patients,incidence of HAI with MDROs was 14.23%(n=119).15 variables that were statis-tically significant in univariate analysis were included in logistic multivariate analysis,the results showed that the following variables entered into logistic regression equation:length of ICU stay (OR,2.493 [95%CI ,1 .816 -3.494]),underlying diseases (OR,1 .536 [95%CI ,1 .243 - 1 .898 ]),hypoproteinemia (OR,87.211 [95%CI , 36.165-210.304]),ventilator days (OR,1 .723 [95%CI ,1 .399-2.121 ]),fever(OR,20.639 [95%CI ,3.462 -123.043]),and primary pulmonary infection (OR,0.295 [95%CI ,0.133 -0.664]).Evaluation of model effect:sensitivity 95%,specificity 87.9%,the area under ROC curve 0.973.Conclusion Logistic regression model has a high goodness of fit in predicting HAI among ICU patients.

Objective To study the effects of Qilongtian on interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in hypoxic pulmonary hypertension (HPH)rats.Methods All male rats were ran-domly divided into six groups:normal group,model group,bosentan group,Qilongtian high-,mid-and low-group.Except the rats in normal group,those in other five groups were under atmospheric hypoxia to establish HPH model.From the first day of modeling,all medication groups were i.g corresponding drugs for consecutive three weeks.Pulmonary hemodynamic parameters were detected by right heart catheteriza-tion under direct vision,mRNA expression of IL-1βand TNF-αin lung tissue were measured using ELISA,protein expression of IL-1βand TNFαin lung tissue were measured using immunohistochemistri-cal methods.Results Qilongtian and bosentan significantly reduced the level of mean pulmonary arterial pressure (MPAP),IL-1βand TNF-αexpression in HPH rats,and the effect of Qilongtian high dosage was stronger with the dose-dependence.Compared with normal group,expression of IL-1βand TNF-αmRNA and protein increased,and those in model group and bosentan group did significantly(P <0.01). Compared with the model group,there was no significant difference in bosentan group (P >0.05),the expression level of IL-1βand TNF-αwere significantly downregulated in all Qilongtian groups (P <0.01 or P <0.05).Compared with the bosentan group and all the Qilongtian groups,there were significant differences,and the Qilongtian high-,mid-dose group had more impact (P <0.01 or P <0.05).Con-clusion High,middle and low dosage of Qilongtian reduced pulmonary hypertension,with high dosage prior to bosentan.The mechanism of lower blood pressure may be via downregulating the expression of IL-1βand TNF-α,but it would be influenced by other vascular tension factors and angiogenic factors.

Background and objective Uridine-diphosphoglucuronosyl transferase 1A1 (UGT1A1),UGT1A1 *28 polymorphism can reduce UGT1A1 enzymatic activity,which may lead to severe toxicities in patients who receive irinotecan.This study tries to build a fragment analysis method to detect UGT1A1 *28 polymorphism.Methods A total of 286 blood specimens from the lung cancer patients who were hospitalized in Guangdong General Hospital between April 2014 to May 2015 were detected UGT1A1*28 polymorphism by fragment analysis method.Results Comparing with Sanger sequencing,precision and accuracy of the fragment analysis method were 100％.Of the 286 patients,236 (82.5％ harbored TA6/6 genotype,48 (16.8％) TA 6/7 genotype and 2 (0.7％) TA7/7 genotype.Conclusion Our data suggest hat the fragment analysis method is robust for detecting UGT1A1 *28 polymorphism in clinical practice.It's simple,time-saving,and easy-to-carry.