Objective To explore the clinical feasibility of three-port laparoscopic cholecystectomy(LC).Methods From April 2006 to April 2007,364 patients with acute or chronic cholecystitis underwent LC using three-port method.The clinical data of the patients were analyzed retrospectively.Results All of the 364 patients were cured.Three-port LC were completed in 357 of them with a success rate of 98%.Seven patients were converted to four-trocar LC because of frozen Callot's triangle and gallbladder atrophy.The operation time was 30-80 min(mean,55 min).No severe complications including bile duct injury,bile leakage,and hemorrhage,occurred in our patients.Among the patients who were treated with three-trocar LC,250 were followed up for 1 to 13 months with a mean of 5 months.During the follow-up,no bile duct stenosis,gallbladder pouch,or residual stones were found.Conclusion Three-port LC is safe,effective,and feasible for patients with cholecystitis,if the surgeons have grasped the techniques of four-port LC.