OBJECTIVE To explore the nursing supervision experience in laminar air-flow operating room(OR) and to provide a very clean and safe operating environment for the surgical patients,in order to prevent the operation infection and raise operation quality.METHODS To take the measures of aseptic,clean,purificatory management,and compare the ratio of infection of incisional wound on aseptic operation and the air bacteria detection results with the general operating room simultaneously.RESULTS The ratio of infection of incisional wound on aseptic operation was 0.05% in the laminar air-flow OR(group A) and 0.15% in the general operating room(group B),P

OBJECTIVE:To prepare chloramphenicol and ephedrine nasal gel and establish its quality control method.METHODS:Gel was prepared with chloramphenicol and ephedrine hydrochloride as principal agents and with carbomer-940 as base.The contents of the principal agents were determined by HPLC and the stability of the gel was investigated as well.RESULTS:The preparation was transparent colloid.The linear ranges of chloramphenicol and ephedrine hydrochloride were 62.5～1 000 and 31.25～500 ?g?L-1(r=0.999 9),respectively,with average recovery rates at 99.64%(RSD=0.5%)and 99.57%(RSD=0.7%),respectively.Storing under room temperature,no obvious change was noted for the property of the sample at 12 months.CONCLUSION:The preparation technique of the gel is simple and feasible,and its quality is stable and controllable.

Objective To investigate the correlation between disability acceptance and personality trait in patients with cerebral infarction, so as to provide a new perspective to improve the disability acceptance. Methods A total of 205 patients with cerebral infarction were investigated by the Acceptance of Disability Scale and the Eysenck Personality Questionnaire-revised-Short Scale for Chinese( EPQ-RSC) . The level of personality trait in patients was compared with the norm, and the Pearson correlation analysis and hierarchical regression analysis were used to explore the effect of personality trait on the disability acceptance. Results The total score of disability acceptance was (128. 08 ± 28. 89) in patients, and 57. 07% of them (117/205) were in the low level, 27. 80% of them (57/205) were in the medium level, 15. 13% of them (31/205) were in the high level. The level of neuroticism, psychoticism and lie in personality trait were significantly higher than those of the norm (t=4. 143,12. 622, 6. 604, respectively;P<0. 01). The level of introvert or extrovert character in personality trait was significantly lower than that in the normal population (t= -9. 552,P<0. 01). Pearson correlation analysis showed that each factor of disability acceptance was significantly negatively correlated with the scores of neuroticism, psychoticism and dissimulation in personality trait (r= -0. 436 0. 025,P<0. 05), but significantly positively correlated with the score of introvert or extrovert character in personality trait ( P<0. 05). Hierarchical regression analysis showed that dissimulation, introvert or extrovert character and neuroticism in personality trait could explained 30. 50% of the total variance of disability acceptance in patients with cerebral infarction (P <0. 05). Conclusions The level of disability acceptance is in the low level in cerebral infarction patients, and personality trait is the influencing factor. Nursing staff can start from the personality trait to improve the level of disability acceptance in cerebral infarction patients.

Objective To evaluate the microbial contamination levels of 10 kinds of Chinese herbal pieces,and explore the diversity of fungi and heat-resistant bacteria.Methods The total aerobic microbial count(TAMC),total yeast and mould count(TYMC),total heat-resistant microbial count(THRC)and 3 types control bacteria were detected according to the microbial limit inspection method of Chinese Pharmacopoeia 2020.Besides,the community characteristics of contaminated fungi and heat-resistant bacteria on prepared slices were analysed based on internal transcribed spacer(ITS)and 16S ribosomal DNA(16SrDNA)high-throughput sequencing method.Results The TAMC of 28％(14/50)samples exceeded 106 cfu/g,the TYMC of 20％(10/50)samples exceeded 104 cfu/g,the THRC of 68％(34/50)samples were higher than 10 cfu/g,and the bile-tolerant gram-negitive bacteria were detected in 40％(20/50)of the samples.Escherichia coli were found in 2 batches of the Pogostemon cablin,but Salmonella was not found in any sample.High throughput sequencing results showed that the fungi contaminated distributed in 12 phylums,777 genera and 1 467 species;the heat-resistant bacteria were mainly distributed in 5 phylums,57 genera and 74 species.The decoction pieces contained Aspergillus flavus and heat-resistant pathogens such as Bacillus amyloliquefaciens,Staphylococcus lentus,and Staphylococcus warneri.Conclusion The 10 kinds of decoction pieces are generally contaminated by microorganisms,and some of them contain toxic fungi and pathogenic heat-resistant bacteria,which poses a potential risk of microbial pathogenicity for patients.

Objective This study aims to investigate the therapeutic effect and mechanism of Naoxinqing on non-alcohol fatty liver disease (NAFLD) induced by a high-fat diet through network pharmacology,molecular docking and in vitro and in vivo experiments. Methods ApoE-/-mice were given a high-fat diet for 12 weeks to establish the NAFLD model,followed by a 12-week Naoxinqing administration. To evaluate the therapeutic effect of Naoxinqing on NAFLD induced by a high-fat diet,biochemical and histopathological experiments were performed,including assessment of blood lipids,liver function,serum inflammatory factors,as well as Hematoxylin and eosin (HE),Oil red O,and Sirius red staining of liver. Subsequently,network pharmacology and molecular docking techniques were employed to predict the key targets of Naoxinqing. Finally,the mechanism of Naoxinqing was validated by Western Blot in HepG2 cells and liver tissue. Results The results of serum biochemistry and liver tissue pathology showed that Naoxinqing can significantly improve high-fat diet-induced hepatic lipid accumulation,hepatocellular injury,and inflammation. Network pharmacology and molecular docking analysis results suggested that Naoxinqing may affect lipid metabolism through the AMP-activated protein kinase (AMPK)/Sirtuin 1 (SIRT1) pathway. Finally,in vitro cell experiment confirmed that the main mechanism of Naoxinqing is to activative the AMPK/SIRT1 pathway,upregulate the expression of downstream carnitine palmitoyltransferase 1 (CPT1A),promote fatty acid oxidation,and ultimately improve NAFLD. Conclusion This study demonstrated that Naoxinqing improved NAFLD by promoting fatty acid oxidation through the activation of the AMPK/SIRT1 pathway.