Objective To study the expression of homocysteine induced gene(HCY-2) in the normal and abnormal human embryonic heart. To research the roles of HCY-2 expressing protein in the human embryonic ventricular myocardial cell development and differentiation. Methods Immunohistochemistry and the technique of picture assay were used to detect HCY-2 expression in the normal and abnormal human embryonic heart. Results HCY-2 expresses during the development of human embryonic heart and mainly in the myocardial cells.The expression increases in the congenital malformation.Conclusion HCY-2 expression protein affects the development of human embryonic heart through affecting the myocardial cells proliferation and the abnormal expression has close relation to the congenital malformation.