Objective:Apoptosis inhibitor 6(Api6),also known as AIM(Apoptosis Inhibitor expressed by Macrophage)or SP?(Soluble Protein alpha),is a newly defined member of the group B scavenger receptor cysteine-rich(SRCR)superfamily.Previous studies have suggested its important roles in immune system regulation,but the involvement of Api6 in lipid metabolism is seldomly studied.Methods:The aim of this study was to define the expression pattern of Api6 in lipid loading and inflammatory macrophages.The human monocyte/macrophage lineage THP-1 cells and murine Raw264.7 macrophages were incubated in the presence of 100 ?g/ml oxidized low-density lipoprotein(oxLDL)alone,LDL plus 200 ng/ml lipopolysaccharide(LPS)and LPS alone.Results:Foam cell formation after treatment was evidenced by oil red O staining.Real-time PCR results showed that after LPS or oxLDL treatment,the mRNA levels of Scavenger receptor SRA were increased 6-fold,2.5-fold and 6-fold,44-fold in Raw264.7 cells and THP-1 cellls respectively,but the expression of Api6 in Raw264.7 did not change.The mRNA levels of Api6 increased 2.7-fold after LPS and oxLDL treatment in THP-1 cells.Conclusion:The expression levels of Api6 in these macrophages were highly related to the presence of nuclear receptor liver X receptor(LXR)isoform LXR?.This study revealed that due to the lack of LXR? isoform,the basic expression level of Api6 was low in Raw264.7 macrophages,that makes it an ideal cell line for further study related to pathophysiological function of Api6 in lipid metabolism.

Cloud computing possesses the great, safe and reliable ability to store data, implements the integration and sharing of information resources, and reduces the economic cost.The infringement risks of intellectual property in digital library under cloud computing environment were described , such as infringement risks of intellectual proper-ty due to cloud computing safety , direct and indirect infringement risks of intellectual property of cloud service pro-viders.Measures for how to prevent and control them were put forward in aspects of laws and administrations.

SoLoMo in the mobile Internet time has provided a new way for service marketing in medical library, its meaning and its effect on medical library and how to innovate SoLoMo marketing service model in medical library were thus described in this paper.

Objective To observe the clinical efficacy of ear acupuncture combined with mirtazapine in the treatment of insomnia.Methods Sixty-two patients with insomnia diagnosed by CCMD-Ⅲ were randomly divided into two groups,31 patients in each group.The patients in the observation group were treated by ear acupuncture combined with mirtazapine,and the patients in the control group were treated by mirtazapine.The clinical efficacy was evaluated by SDRS after 4 weeks of treatment.Results The SDRS score of the observation group significantly decreased after one week of treatment [(13.40±5.79)points vs.(21.79±6.12)points,t=2.204,P＜0.05].The SDRS score of the control group significantly decreased after one week of treatment [(14.68±5.88)points vs.(22.67±6.06)points,t=2.198,P＜0.05].After 4 weeks of treatment,the clinical efficacy of the observation group was significantly better than that of the control group (z=-2.209,P＜0.05),the SDRS score of the observation group was also significantly better than that of the control group (t=2.163,P＜0.05).Conclusion Ear acupuncture combined with mirtazapine has better effect in the treatment of insomnia patients,it has less side effects and lasting effects.

Exhaled nitric oxide fraction (FeNO)reflects eosinophilic bronchial inflammation,and has been widely used to assist in the diagnosis of asthma,to monitor the airway inflammation and the responce to treatment effects of asthma in recent years.FeNO as a noninvasive examination,obtains a high degree of cooperation in adults and children over 5 years of age,but there are a lot of problems in children under 5 years of age.At present,there is still a lack of normal values and large sample-sized studies of FeNO in infants and neonates in China.The principle and method,normal reference value,influencing factors,clinical application and so on of exhaled nitric oxide detection are reviewed in this paper.

Objective To investigate the relationship between plasma N-terminal pro brain natriuretic peptide(NT-proBNP) level and left ventricular(LV) diastolic dysfunction in hypertensive patients without systolic dysfunction.Methods Forty-four cases of primary hypertensive patients with left ventricular ejection fraction more than 50％ were enrolled,14 cases in normal diastolic function group,30 cases in diastolic dysfunction group(18 cases in delayed relaxation group and 12 cases in pseudo normalization filling group).LV diastolic function was assessed with conventional Doppler by means of mitral inflow and with tissue Doppler echocardiography by means of mitral annulus.The ratio of early diastolic transmitral E wave velocities to tissue Doppler mitral annulus early diastolic E' wave velocities (E/E') was used to detect LV filling pressures.Patients were divided into three groups according to E/E' ratios.Plasma concentrations of NT-proBNP were also measured.All related data were statistically analyzed.Results The levels of NT-proBNP were (115.2 ± 78.9)ng/L in LV diastolic dysfunction group,(34.7 ± 17.5) ng/L in normal filling group and (206.6 ± 75.8) ng/L in pseudonormal filling group.The level of NT-proBNP significantly elevated (F =85.263,P =0.000) along with the severity of lefi ventricular diastolic dysfunction.There was significant difference between each of the two groups among the three groups(all P ＜0.01).According to E/E' ratios,the patients were divided into three groups:E/E' ＜10(n =16),10 ＜ E/E ＜ 15(n =18),E/E' ＞ 15(n =10).The level of NT-proBNP were (39.5 ± 20.1) ng/L,(71.2 ± 26.4) ng/L and (203.7 ± 75.2) ng/L respectively in the three groups.The NT-proBNP blood level was positively correlated significantly with E/E' ratio (r =0.80,P ＜ 0.01).The sensitivity was 87％ and the specificity was 100％ for diagnosis of left ventricular filling pressure (E/E' ＞ 15)according to a NT-proBNP value of 119 ng/L.Conclusion Combining of plasma NT-proBNP and Doppler E/E'have potential value for identification of those patients with hypertension to detect early cardiovascular changes,especially LV diastolic dysfunction.

Adult ; Calcinosis ; metabolism ; pathology ; Diagnosis, Differential ; Fibroma ; metabolism ; pathology ; Gastrointestinal Stromal Tumors ; pathology ; Humans ; Male ; Stomach Neoplasms ; metabolism ; pathology ; Vimentin ; metabolism

Objective To investigate the relationships between serum inflammatory mediators and myocardial injury in rats with sepsis.Methods Thirty male Sprague Dawley (SD) rats were randomly divided into normal control group and sepsis (0,24,48,72 h) groups.The rats subjected to sepsis were induced with cecum ligation perforation (CLP).Tumor necrosis factor alpha (TNF-α),interleukin-6 (IL-6),cardiac troponin Ⅰ (cTnI) and B-type natriuretic peptide precursor (NT-proBNP) in the venous blood were measured at 0 h,24 h,48 h,and 72 h after CLP.The lactic acid (Lac) of the arterial blood,cardiac index (CI),acute physiology and chronic health evaluation (APACHE) Ⅱ score were also determined simultaneously.Results The levels of TNF-α,IL-6,cTnI,NT-proBNP,and Lac in sepsis (24 h,48 h,and 72 h) groups were significantly elevated compared to normal control and sepsis (0 h) group.In addition,the number of sepsis (24 h,48 h,and 72 h) groups progressively increased (P ＜0.05).Conclusions It existed a close relationship between serum inflammatory mediators and myocardial injury,and made myocardium damage after CLP.

Objective To investigate the effect of transverse incision surgery for epicanthoplasty with double eyelid fold reconstruction. Design Retrospective case series. Participants 103 patients with epicanthus. Method Transverse incision was applied for epicanthoplasty in all patients, and incorporated with double eyelid fold reconstruction. Patients were followed up 3-6 months. Main Outcome Measures Size of lacrimal caruncle, length of palpebral fissure, inner canthic diameter and scarring degree. Results Lacrimal caruncle was revealed 3/4 merous in 21 cases, 2/3 merous in 56 cases, 1/2 merous in 8 cases. Extent of palpebral fissure was 30-31mm in 5 cases, 28-29 mm in 69 cases, 26-27 mm in 11 cases. Inner canthic diameter was 32-33mm in 61 cases, 30-31 mm in 23 cases, 29 mm in 1 case. The scarring was little in 56 cases, obviously in 29 cases. Epicanthus relapsed in 2 cases. 16 cases lost follow-up. Conclusion Transverse incision surgery for epicanthoplasty with double eyelid fold reconstruction has less tissue damage and minimal scarring produced at the medial cantha. It is a simple and reliable surgical approach for correction of epicanthus.

Objective: To investigate the regulatory effect of lumbrukinase CI.RK) on the gastric cancer SGC7901 Cells, and to clarify its mechanism Methods: The SGC7901 cells in the logarithmic growth phase were selected and divided into control group and 2, 4 , 8 U • ml. I.RK groups. MTT assay was used to detect the inhibitory rates of proliferation of SGC7901 cells in various groups in vitro at different time (24, 48 and 72 h). Cell scratch assay was used to detect the migration abilities of the SGC7901 cells in vitro in various groups. Flow cytometry was used to determine the apoptotic rates of SGC7901 cells and the pencentages of cells at different cell cycles in various groups. The expression levels of Bcl-2, Rax, and caspase-3 in the SGC790I cells in various groups were detected by Western blotting method. Results: The results of MTT assay showed that compared with control group, the inhibitory rates of SGC7901 cells in different doses of I.RK groups after treated for 24, 48 and 72 h were increased ( P<0.01). The cell scratch assay results showed that compared with control group, the migration distances of SGC790I cells in 4 and 8 U • mL"1 I.RK groups were increased significantly ( P