Objective:To study combination AdCD-UPRT suicide gene mediated on lung cancer A549 in vitro. Methods:The recombinant AdCD-UPRT adenovirus was packaged and generated,and the title of virus was measured. The efficiency of transfecting target cells was detected,the toxicity of virus was measured,and the expression of CD-UPRT gene in A549 cells was assayed. The cytotoxicity and the bystander effect on A549 cells were measured by MTT assays respectively. The apoptosis was analyzed by AO-EB fluorescent staining,transmission electron microscope and flow cytometry. Results:The combination AdCD-UPRT had obviously growth inhibition effect on A549 cell and bystander effect. The cells were blocked in S phase and induced into apoptosis. Conclusion:The combination AdCD-UPRT suicide gene had cytotoxicity on lung cancer A549 in vitro.

Highlights of myelodysplastic syndromes(MDS)in the 18th congress of the European Hematology Association(EHA)were reviewed.Some new discoveries about the spectrum of gene mutations,prognosis and treatment of MDS patients were reported.

Secretome includes all the gene products secreted by a cell. The development of proteomicbased approaches and genome-based computational prediction technology have gradually revealed secretome plays an important role in pathogen infection recently. Secretome facilitates pathogen to proliferate and invade the host by inhibiting the host's immune system, resisting acidic PH conditions, snatching nutrition and so on.Identification of pathogenic secretome helps discovering new drug targets and biomarkers, and supporting the diagnosis and treatment.

Magnetic resonance imaging(MRI) has multi-faceted advantages due to its non-ionization radiation, excellent tissue contrast, high spatial resolution and multi-dimensional imaging technique. It can clearly observe the pathological changes of deseases, and judge for the prognosis. With the development of MRI, MRI has become increasingly popular in the diagnosis of fetal malformation. In this paper, it is described the value of MRd in the diagnosis, management and prognosis of fetuses malformations, via the central nervous system, circulatory system, respiratory system, digestive system, genitourinary system, musculoskeletal system, endocrine system, and so on.

OBJECTIVE:To study the effect of moxifloxacin on pharmacokinetics of phenytoin sodium in rabbits. METHODS: A total of 10 rabbits were injected with phenytoin sodium (10 mg?kg-1) via the vein in ear edge,with serum samples taken at 10,30,60,120,240,360,and 480 min,respectively for establishing phenytoin sodium alone group. 48 h after the last sampling,the rabbits were given oral moxifloxacin (10 mg?kg-1) for 5 consecutive days;on day 6,the rabbits were injected with phenytoin sodium (10 mg?kg-1) at 2 h after oral administration of moxifloxacin,then serum samples were taken at different time points for establishing combination group of phenytoin sodium and moxifloxacin. The serum concentrations of phenytoin sodium at different time points were determined by UV-spectrophotometry and the pharmacokinetic parameters were computed with 3p97 program. RESULTS: The plasma concentration of phenytoin sodium reduced in the combination group compared with alone group. The AUC in the two groups was (5 836.22?489.13) vs. (4 329.21?344.67) mg?min?L-1 (P0.05). CONCLUSION: The elimination of phenytoin sodium was significantly accelerated by combination with moxifloxacin.

Objective:To analyze one case of drug-induced liver injury to provide reference for clinical pharmacists participating in the clinical practice. Methods: Through the participation in the diagnosis and therapy of one patient with drug-induced liver injury, clinical pharmacists found the correlation between the suspected drugs and the disease, which provided basis for the clinical diagnosis and therapy. Results:Clinical pharmacists actively assisted physicians by providing reasonable defining characteristics and drug therapy scheme for the patient free from drug interactions and adverse reactions. The function of the patient recovered after the treatment. Con-clusion:Clinical pharmacists participating in clinical practice can make use of pharmacy knowledge to search causes of diseases and optimize therapeutic scheme.

Objective To investigate the role of Ginsenosides Rg1 for non-alcoholic fatty liver disease by β-oxidation.Methods 120 SD rats were randomly divided into control group(CON),model group(HFD),Ginsenosides Rg1low,medium and high dose group (GLD ,GMD and GHD) ,sodium deoxycholate of bear treatment group (PDT ) ,20 rats in each group .After 4 and 8 weeks treatment ,the rats were sacrificed ,Pathology of hepatic tissue was tested by HE staining ,and liver function ,lipid levels ,hepatic ac-yl-CoA synthetase (CoASH1) ,carnitine acyl transferase I(CATI) and acetyl coenzyme A oxidase 1 (ACOX1) mRNA and protein expression were tested .Results After 4 weeks of treatment ,the liver function tested by HE staining only improved in GHD group . After 8 weeks ,there′s a little fat particles aggregation in PDT and GLD groups ,but no infiltration of fat in GMD and GHD groups . After 4 weeks ,AST ,ALT and AKP ,CHOL ,TG and LDL-C levels were significantly lower in PDT ,GLD ,GMD and GHD groups compared with HFD group (P<0 .05) ,which were significant declined 8 weeks later .After 4 weeks ,HDL-C level in four groups was significantly increased ,then reached the normal level 8 weeks later .After 4 weeks ,CoASH1 ,CATI and ACOX1 expressions in hepatic tissue of four groups were significantly increased ,which improved more obviously after eight weeks .Conclusion Ginsen-oside Rg1 can improves nonalcoholic fatty liver phenotype by regulation of β-oxidation .

Objective To compare the efficacy and safety of candesartan cilexetil and enalapril in the treatment of senile hypertension.Methods 100 patients with hypertension were randomly divided into the treatment group and control group according to digital table method.The treatment group were received candesartan cilexetil 4 ～ 8mg once daily for 8 weeks,and control group were received enalapril 10 ～ 20mg once daily for 8 weeks,and the efficacy and adverse reactions were watched.Results The SBP and DBP were (134.42 ± 6.39) mmHg and (82.00 ± 5.05) mmHg for candesartan cilexetil and (137.70 ± 5.27) mmHg and (81.76 ± 5.03) mmHg for enalapril after 8 weeks.A significant reduction of blood pressure was achieved in both groups than before treatment (tcan =35.85,30.88;tena =37.92,31.67,all P ＜ 0.01).After treatment pulse pressure was (56.06 ± 4.91)mmHg for candesartan cilexetil and (60.04 ± 4.40) mmHg for enalapril.Compared with before treatment,pulse pressure was significantly reduced in the treatment group (t =16.93,P ＜ 0.01) and was no significant difference in control group (t =6.34,P ＞ 0.05).The incidence of adverse reactions of patients with treatment group was obviously lower than that of the control group.Conclusion Candesartan cilexetil is effective and safe in senile hypertension patients,and it could be used as a first-line treatment.

12 gestational-week uterus) were treated in our hospital by LAVH. The operation time,intraoperative blood loss,and postoperative recovery of the patients were compared to another 110 cases that underwent TAH. Results The operation time in the TAH group was significantly shorter than that in the LAVH group [(109?27) min vs (130?22) min,t=-6.441,P=0.000]. During the operation,the LAVH group had a mean of (121?70) ml blood loss,which was significantly lower than that in the TAH group [(141?73) ml,t=-0.903,P=0.368]. After the operation,the patients in the TAH group had longer recovery time for gastrointestinal function and longer hospital stay than those in the LAVH group [(45?13) h vs (24?6) h,t=15.778,P=0.000;and (7?2) d vs (5?2) d,t=7.530,P=0.000]. Conclusions Compared to TAH,LAVH results in shorter hospital stay and fewer postoperative complications. It is a feasible and safe treatment for large uterus.

OBJECTIVE To study approaches to effective infection management,so that it can function high effectively.METHODS Some important facts were reviewed,analyzed and summarized,which had influence on hospital infection management.RESULTS The quality and knowledge of the team in charge of infection management,the organization and regulations concerned were prerequisite to effective infection management,with reasonable incentive means.CONCLUSIONS Analysis and much emphasis to be given on the facts affecting hospital infection management are key points to its improvement.