Objective:To obtain the spike glycoprotein(S protein) of SARS coronavirus (SARS-Cov) for detection of the corresponding antibody and preparation of diagnostic kits.Methods:Genes encoding S protein were picked out to form a new gene and divided into two parts according to the prediction of antigenicty.They were obtained by nucleotide synthesis and were cloned into expression vector pET32a(+) respectively.Proteins then were induced by IPTG and purified through Ni-NTA.Results:Target proteins were successfully expressed and purified.Conclusion:The recombinant S proteins of SARS-CoV could be of great help in diagnosing the disease and investigating the virus further.

It is well established that renin-angiotensin system (RAS) is the major regulatory networkthat maintains blood pressure, fluid and electrolyte balance and the homeostasis of cardiovascular system.Most studies in the last decades centered on the pivotal members of RAS, that is, angiotensinⅡ (AngⅡ) and angiotensin converting enzyme (ACE). Recent identification of ACE-2, the homology of ACE,and the identification of the multiple products degraded from angiotensinⅠ, including AngⅢ, AngⅣ,Ang1 -9, Ang1 -7, Des-Asp-angiotensinⅠ(DAAⅠ), etc, have proved that AngⅡ is not the only bio-logical active compound of RAS. Peptides derived from angiotensinogen,that is, family of angiotensins,display independent and pleiotropic biological activitiesin vivo, and interact with each other both in me-tabolism pathway and the biologic effects. The imbalance of the network of angiotensin metabolites exhib-its significant pathophysiological role in cardiovascular disease.

Treating autoimmune diseases(AID) is a challenge for physicians.Traditionally,we use cortisone or other immune inhibitors to treat AID although we know that these kinds of drugs can cause some severe toxic action on patients if used longer.For over ten years,the biological therapy for AID,which specially targets to the pathogenesis of AID,has long been engaged in the study.These new drugs can protect the normal immune system while stopping undesirable immune reaction to the target organ.

Objective To investigate the application of the voice acoustic analysis in the evaluation of curative effects of different chronic laryngitis.Methods The adult patients with chronic laryngitis (80 cases)were divided into 3 different syndrome types what were as the Yin deficiency group (28 cases),the Qi deficiency group (25 ca-ses),and the TanNing blood stasis group (27 cases).According to the different compositions of golden voice series medicines,the medicines were administered,combined in two to three.A total of 30 normal people were set to the control group.The German ATMOS voice test and analysis system was used to detect continuous vowel signals and the results were compared before and after treatment.Results Before the treatment,the chronic laryngitis group, compared with control group,had higher jitters,shimmers and NHR.The MPT was significarry shorter and DSI smaller compared with the control group(P <0.05).After 1 month of medication,the Yin deficiency group was the best and there were no significant differences in almost all parameters compared with control group (P >0.05)ex-cept for the parameter-jitter.The jitter,shimmer,and NHR decreased significantly after 1 month treatment while the MPT and DSI increased significantly in the Yin deficiency group and the Qi deficiency group compared with be-fore treatment (P <0.05).There was a statistically significant difference on jitter,shimmer,NHR and DSI and no difference on MPT compared with before treatment in TanNing blood stasis group.Conclusion The voice acoustic a-nalysis could be objective and quantitative to analysis the chronic laryngitis patients'voice quality with different syn-drome types and evaluate the curative effects.

ObjectiveTo investigate the clinical application of TPO-Ab by comparing the relationship between TPO-Ab and TMAb in ATD. MethodsIn this study with the method of RIA, we measured the serum level of TPO-Ab and TMAb in 200 ATD patients, 50 patients with non-ATD and 30 normal controls, and analyzed the relationship of TPO-Ab and TMAb, compared the differ ence between TPO-Ab and TMAb for diagnosis ATD. ResultsTPO-Ab titer increased in ATD es pecially in Hashimoto's thyroiditis(P＜0.01), at the same time it could influence the state of the throid function. A significant positive correlation was found between TPO-Ab and TMAb(γ= 0.83,P＜0.01 ), but TPO-Ab appeared to be more sensitive and specific for diagnosising Hashimoto's thy roiditis than TMAb. ConclusionTPO-Ab is a sensitive and specific stantard for diagnosising ATD.

Objective To explore curative effects of coracolavicar ligament reconstructed with artificical tendon on treatment of complete acromioclavicular joint dislocation.Methods 32 patients with complete acromioclavicular joint dislocation received artificial tendon graft, 19 cases of whose implanted flexible carbonfiber tendon,and the others were treated with human-hair keratin artificial tendon (HHKAT). All patients were given bu internal fixation with Kirschner pins for 6 weeks after the operation.Results Fellowed-up for 4 months to 30 months showed the acromiclavicular join reduction and recovery of the anatomical relation of acromioclavicular joint checked by X-rayrecovered. According to karlsson scores, 21 cases graded excellent, 10 cases good and only one case bad, the excellent and good rate of recovery was 96 9%.Conclutions With the internal fixation with Kirschner pins in short term,and plus artificial tendon reconstructing coracoclavicalar ligament for patients with complete acromioclavicular joint dislocation, which had a little wound and a good curative effect, was easy to be performed and have worth in clinical application.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Female ; Humans ; Tremor ; therapy

Occupational Diseases ; prevention & control ; Occupational Health Services ; Public Health

Objective To explore the influence of the fetal microchemirism in the thyroid tissue on the Graves' disease(GD) animal model.Methods Mice in experimental group were injected with Ad-TSH289(5?108IU) to construct the GD animal model,and when the immunization was over,the experimental group was randomly divided into three parts again: two groups were mated with male mice until two weeks of pregnancy or two weeks post-partum while the other group was simple immunized with Ad-TSH289 only.SRY was tested by real-time PCR.Thyroid tissue was stained with HE for pathological examination.Results Histological examination of the thyroid gland revealed that 61.8% of simple immunized mice had goiter,the success rate of GD animal model was 38.5% in pregnancy group,but 76.9% in postpartum groups(P

Objective To explore the effect of endoplasmic reticulum stress (ER stress) in uric acid?induced phenotypic change in renal tubular epithelial cells (HK?2). Methods (1) HK?2 cells were cultured with 0, 75, 150, 225, 300 mg/L uric acid for 24 h in vitro. (2) The cells were divided into normal control group, ER stress inhibitor 4?PBA (5 μmol/L) group, uric acid (150 mg/L) group and 4?PBA+uric acid group for 24 h. Morphological changes of HK?2 cells were observed under inverted microscope. MTT assay was used to detect the proliferation of HK?2 cells treated with 150 mg/L uric acid for 24, 48 and 72 h. The protein expressions of α?smooth muscle actin (α?SMA), vimentin, snail, glucose regulated protein 78 (GRP78) and the phosphorylation of eukaryotic initiation factor 2α(p?eIF2α) in HK?2 cells were measured by Western blotting. Results Compared with the control group, HK?2 cells in uric acid groups (150, 225, 300 mg/L) showed fibroblast?like appearance. The protein expressions of α?SMA, vimentin, snail, GRP78 and p?eIF2α in 150 mg/L and 225 mg/L uric acid groups were higher than those in the control group (all P<0.05). The proliferation of HK?2 cells in 150 mg/L uric acid group was lower than that in control group at 48 and 72 h (all P<0.01). Compared with the uric acid group, the cell morphology in 4?PBA+uric acid group was improved, and the protein expressions ofα?SMA, vimentin, snail, GRP78 and p?eIF2α were decreased (all P<0.05). Conclusions Uric acid may induce the phenotype transformation of renal tubular epithelial cell, and ER stress is involved. 4?PBA may inhibit the uric acid?induced ER stress response and phenotypic transformation, and may be beneficial in attenuating uric acid?induced renal tubular damage.