Objective:To observe the effects of different low intensity pulsed ultrasound(LIPUS) on proliferation of fibroblast and to screen out the optimum parameters of LIPUS.Methods:LIPUS of different frequency(1?1.2?1.7MHz)and different intensity was used to stimulate the cultured human fibroblast cells for 5 min,10 min,20 min and 30min,respectively,and examination of cell proliferation by MTT after 24h.Results:①Optical absorption value of cell activity in MTT assay increased significantly in the cell culture with LIPUS of different frequency(1?1.2?1.7MHz) and four intensity of each frequency treatment for 5,10 minutes,as compared with the control group(P

Objective To investigate the therapy of chronic eczema patient by mounting method withshen que point,and its effect to relieve itch fast and increase the comfort and contentment of patients.Meth-otis 68 caiges of chronic eczema were randomly enrolled into the experimental gttmp(38 cases)and the controlgroup(30 cases).The experimental group added mounting method with shen ClUe point to normal therapy in-observed the beginning time of itch remission and the therapeutic effect of two groups after 14 days.ResultsCompared with the control group,We found that itch remission in the experimental group wok effect earlier thanthat of the control group,P<0.05.and the therapeutic effect in the experimental group wag significantly differ-ent from that ofthe control group after 14 days.P<0.05.Conclusions The mounting method with sben quepoint can relieve pruritus of patients with chrunie eczema effectively and promote therapeutic effect.

Actins ; immunology ; metabolism ; Antibodies, Monoclonal ; analysis ; Antigens, CD34 ; immunology ; metabolism ; Breast Neoplasms ; metabolism ; Female ; Humans ; Immunohistochemistry ; methods ; Lung Neoplasms ; metabolism ; Membrane Proteins ; immunology ; metabolism ; Tumor Suppressor Protein p53 ; immunology ; metabolism

Objective To investigate the efficacy of transorally inserted anvil system(OrVilTM)in esophagogastrostomy and esophagojejunostomy after laparoscopic gastrectomy.Methods The clinical data of 34 patients with gastric neoplasms who were installed OrVilTM for esophagogastrostomy or esophagojejunostomy at the Changhai Hospital from July 2009 to February 2011 were retrospectively analyzed.After radical dissection of lymph nodes and full mobilization of esophagus,the esophagus was transected and the anvil was then transorally inserted into the esophagus by using the OrVilTB system.Double-stapling esophagogastrustomy or esophagojejunostomy with a circular stapler Was performed intracorporeally under direct laparuscopic view.Results The surgery was success fully completed in all the 34 patients with no conversion to open surgery.Two patients had difficulty in placing OrVilTM system.and the condition Was alleviated by reducing tension in the cuff and tilting the head back.The mean operation time,volume of blood loss,duration of postoperative hospital stay and time to gastrointestinal function recovery were 175 minutes(range,90-240 minutes),196 ml(range,50-800 ml),7.6 days(range,5-14 days)and 3 days(range,2-6 days).No postoperative anastomotic leakage Was detected.Thirty-three patients were followed up for 2-20 months with a mean time of(10±6)months,and no tumor recurrence or metastasis occurred.Conclusion OrVilTM system changes insert direction of the anvil,which significantly reduces the difficulty of laparoscopic operation,shortens the operation time and avoids the thoracotomy.

0.05). CONCLUSION:The treatment of the Tile C type pelvic fracture with the percutaneous iliosacral articulation screws and pelvis external fixation under X-ray perspective has the advantages including small wound,simple operation,reliable fixation,short operation time,quick reactivating and low operation infection rate.

Objective To investigate the clinical and imaging features of cerebral cortical vein thrombosis (CoVT). Methods The clinical data of 13 inpatients with CoVT were enroled from September 2010 to September 2015. The etiology, clinical symptoms, imaging features, and differential diagnosis of CoVT w ere summarized and analyzed. Results Among the patients, there w ere 7 females (53.85%) and 6 males (46.15%). Their age ranged from 18 to 59 years (mean 34.23 ±12.08). The most common clinical manifestations were headache (9 patients) and seizures (9 patients). The 13 patients had cerebral parenchymal involvement and they al received anticoagulant therapy. Conclusions The clinical symptoms of CoVT are various. Seizures and headache are the most common symptoms. Most of patients are accompanied by brain parenchymal lesions and venous sinus thrombosis. When patients have headache and seizures, and head images suggest hemorrhagic infarction, imaging examination of the improvement of the cerebral venous system thrombosis is recommended.

Multidrug resistance is a common form of drug resistance of tumors and one main reason for chemotherapy′s failure and recrudescence of acute leukemia. In recent years,substantial studies addressed the different aspects about MDR1,including MDR1 genetic polymorphism,mRNA/protein expression as well as the function of its coding protein (P-gp).This paper reviewed recent advances about the relation between MDR1 genetic polymorphism and acute leukemia prognosis with the aim to improve acute leukemia curing and chemotherapy outcome evaluating in China.

The study evaluates the lipolysis rate and extent of type Ⅲ lipid formulations using testosterone undecanoate as a model drug after digestion with in vitro lipolysis model, and studies the digestive regularity with optical microscope and electrical conductivity. The results showed that for testosterone undecanoate type Ⅲ lipid formulations with castor oil as oil phase and Transcutol HP as latent solvent, the lipolysis rate and extent were increased with the increase of oil phase proportion and were decreased with excessive proportion of surfactant, in which can see liquid crystal phase during lipolysis process. The lipolysis rate of type ⅢB lipid preparations with different surfactant were ordered as Labrasol > Tween 80 > Cremophor EL, but the rate of type ⅢA is different in quick digestion phase and slow digestion phase. The lipolysis extent of type Ⅲ lipid formulations with different surfactant were ordered as Cremophor EL > Tween 80 > Labrasol. These may be related to the digestive effect of pancreatic lipase on different surfactants. This study implied that the lipolysis rate and extent of type Ⅲ lipid formulations are greatly influenced by the proportion of oil phase and surfactant, and the surfactant structure. These factors will affect the in vivo digestion and should be taken into account when screening type Ⅲ lipid formulations.

The neurotoxin β-amyloid (Aβ) is the main hallmark of Alzheimer′s disease (AD). Recent evidence suggests that, in common sporadic or late-onset forms of AD, elevated brain Aβ levels are caused by impaired clearance rather than overproduction. The cell surface receptor′s low-density lipoprotein receptor-related protein-1 (LRP1) has been reported to not only play a role in Aβ endocytosis, but also exist in the blood-brain barrier system, peripheral blood, liver, kidney and other tissues and organs, and transport Aβ to the cerebrospinal fluid or blood system by passing through the blood-brain barrier or the blood-cerebrospinal fluid barrier effectively, and finally clear it out of the body through peripheral tissues and organs. In this review, the role of LRP1 in the peripheral transport and clearance of Aβ is described, and it may be a safe and effective way to reduce Aβ in the brain and even improve cognitive dysfunction.

Toll like receptors (TLRs) are the earliest discovered natural immune pattern recognition receptors (PRRs). The abnormality of TLR signal transduction pathway is the key factor leading to chronic inflammatory, cancer, nervous system disease and cardiovascular diseases. The development of TLR agonists and inhibitors has attracted much attention. Currently known TLR2 agonists, such as lipopeptides or their derivatives, have certain limitations in drug development due to their difficult synthesis, easy hydrolysis, and triggering inflammatory cytokine storms, while inhibitors have been rarely reported. New small molecule TLR2 agonists or inhibitors with higher stability are more likely to be developed as tumor immunotherapy or anti-inflammatory drugs.