Objective: To find an effect approach to solve the problem on the management of clinic charge bill. Methods:According to the actual working conditions, it suggests we should enhance the management of clinic charge bill form the attitude, system and measures. Results:According to the method and the microcomputer management referred by this article, the management of outpatient charges and bills will be more effective. Conclusion:The management of outpatient charges and bills should be regarded as an important part of the hospital management, and the microcomputer monitoring management should be used to strengthen and improve the management level.

Objective:In order to discuss the influence of processed methods to the contents of total saponins and asperosaponin Ⅵ in Dipsacus asperoids,the content alternation of total saponins and asperosaponin Ⅵ in crude and processed Dipsacus asperoids was investigated.Methods:The content of total saponins was determined by spectrophotometry using vanillin-acetic acid-perchloric acid as the chromogenic reagent.The content of asperosaponin Ⅵ in crude and processed Dipsacus asperoids was determined by HPLC.Results: Contracted to the crude Dipsacus asperoids,the content of total saponins in the processed is higher.The content of asperosaponin Ⅵ in the processed Dipsacus asperoids is higher than that in the crude significantly.Conclusion:Different processed mothods affected the content alternation of asperosaponin Ⅵ in Dipsacus asperoids.The processing of Dipsacus asperoids is reasonable.

Objective To accesse the psychological situation of Gastroscopiers. Methods Questional investigation face to face had been done to some gastroscopiers by using SCL-90 chart and the results were comapred to the common model. Results There were no obviously diaappiontment between the results of the gastroscopiers’and the common model. In total fraction , total average fraction and all kinds of elements which proved the normal psychological health of these people. That was to say that it’s not the gastroscopyical check who has caused the supper stimulaion or substaintial passive emotion although more bitterness. Conclutions The psychological situation of Gastroscopiers is basical normal.

Pediatric knowledge was not fully learned by seven-year program medical students of pediatrics major in first five years,so two years of postgraduate was the key period for culturing their pediatric specialty.Students of seven-year program were characterized by strong theoretical knowledge but poor pediatric clinic knowledge,therefore,pediatric clinic teaching should emphasize on basic knowledge and skills,avoid dwelling on specific details at early stage,pay attention to their communicative ability with children and their relatives,combine theory with clinic practice and teach from surface to point.Meanwhile,we aimed at changing them into pediatric doctor through cultivating their clinic skills and scientific research ability.

Objective To observe the changes of plasma lipoprotein concentrations after an oral glucose tolerance test and research their relation to insulin resistance in Type 2 diabetic patients.Methods The age,duration of diabetes,sex,waist circumference,hip circumference,waist-hip ratio,height,body weight,body mass index ( BMI ),systolic blood pressure,diastolic blood pressure,fasting plasma glucose,HbAl C,fasting insulin,and fasting and 60-,120-,180-minute post-load plasma glucose,plasma insulin,triglycerides ( TG),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol(LDL-C) and total cholesterol(TC) after a 75-g oral glucose tolerance test were measured in 26 Type 2 diabetic patients.Comparison of plasma fasting lipoprotein concentrations and postload lipoprotein concentrations was made,and the relation between fasting and post-load lipoprotein concentrations and Homa-IR was researched.Stepwise multiple regression analysis was applied to investigate the areas under the lipoproteins' curves over 180 minutes.Results The declination of post-load lipoprotein concentrations after the 75-g glucose tolerance test was statistically significant( all P ＜0.01 ).Plasma TG concentration both in fasting and post-load state was positively related to Homa-IR( all P ＜ 0.05 ),and plasma HDL-C concentration in fasting and post-load state was negatively related to Homa-IR( all P ＜ 0.05 ).However,the correlation between both fasting and post-load state LDL-C and TC concentration and Homa-IR had no statistical significance.Moreover,stepwise multiple regression analysis revealed Homa-IR was the independent factor capable of modulating the area of HDL-C curve over 180 minutes after the 75-g glucose tolernce test ( P ＜ 0.05 ).Conclusion There was a significant declination of plasma lipoproteins after a 75-g glucose tolerance test,and plasma fasting and post-load TG and HDL-C concentrations were related to insulin resistance.Insulin resistance should play a significant role in the post-load HDL-C concentration after 75-goral glucose tolerance test.

Under the high pressure of employment, traditional teaching method that is based on inculcating the knowledge to students does not adjust current condition any more. So, we start to looking for a new, more suitable way of teaching.--Project Based-on Learning, and have analyzed the advantages in health information technology teaching by construction doctrine in combination with the concept and principle in wedlock construction. At the same time we introduce project Based-on Learning in concrete application to find a way to put the theory into practice.

BACKGROUND:Mesenchymal stem cels can differentiate into lung parenchymal cels involved in lung injury repair, providing a new approach for the application of mesenchymal stem cels in patients with chronic obstructive pulmonary disease. OBJECTIVE:To observe the effect of bone marrow mesenchymal stem cel transplantation on the repair of airway injury in rats with chronic obstructive pulmonary disease. METHODS:Twenty-four female rats were randomized into four groups: bone marrow mesenchymal stem cel transplantation group (cel transplantation group,n=12); bone marrow mesenchymal stem cels group (cel control group,n=4); model group (n=4); healthy control group (n=4). Rat models of chronic obstructive pulmonary disease were established in the cel transplantation group and model group using fumigation+lipopolysaccharide method; and at 1 day after modeling, model rats were given 1 mL CM-Dil-labeled bone marrow mesenchymal stem cels and 1 mL PBSvia the tail vein in these two groups, respectively. In addition to tracheal injection of normal saline (300 μL) at 1 and 14 days, rats in the cel control and healthy control groups were given 1 mL CM-Dil-labeled bone marrow mesenchymal stem cels and 1 mL PBSvia the tail vein, respectively. At 1, 7, 15 and 30 days after cel transplantation, lung tissue and serum markers of al rats were detected. RESULTS AND CONCLUSION:(1) Hematoxylin-eosin staining showed that emphysema and airway injury was milder in the cel transplantation group than the model group, but severer than the cel control and healthy control groups. (2) The total number of leukocytes and neutrophils in the peripheral blood was higher in the cel transplantation group than the cel control and healthy control groups (P < 0.05); with time, the total number of leukocytes and neutrophils was decreased gradualy. (3) Compared with the cel control and healthy control groups, the interleukin-10 level in the peripheral blood was lower and the levels of tumor necrosis factor-α and granulocyte colony-stimulating factor were higher at 1 day after cel transplantation (P < 0.05). With time, in the cel transplantation group, the interleukin-10 level was increased gradualy, the level of tumor necrosis factor-α was decreased gradualy, and the level of granulocyte colony-stimulating factor was increased first and then decreased, which was highest at 7 days after cel transplantation. (4) Partial CM-Dil-positive cels were positive for CC16. Taken together, bone marrow mesenchymal stem cel transplantationvia the tail vein can improve lung injury of rats with chronic obstructive pulmonary disease, and it is involved in the repair of airway injury through differentiation into epithelial cels and immune regulation.

Objective: To improve the standardization method for ethanol potassium hydroxide volumetric solution. Methods: A new potentiometric titration method was established and compared with the indicator method described in Chinese Pharmacopeia (2010 edition) . Results:There was no significant difference between the results of the potentiometric method and the indicator method( P>0. 05). Furthermore, the potentiometric titration had significant jump range, good precision and high reliability. Conclusion:The po-tentiometric method can be used in the standardization of ethanol potassium hydroxide volumetric solution.

Scutellarin (SC),a botanical extract,possesses a variety of suppressing free radical generation,anti-inflammatory,cardioprotection and anti-virus activity.Recently,many studies show that SC has inhibitory effects on the growth of many tumors and is a kind of potential anti-tumor drug.Its anti-tumor mechanisms mainly include inducing apoptosis,arresting cell cycle,enhancing the adhesion between cells,inhibiting tumor invasion,reversing multidrug resistance,anti-angiogenesis and so on.

Objective Working memory (WM) is one of important cognitive functions.The aim of this study was to investigate spatial distributions of electroencephalographs (EEGs) power during visual WM task,which may provide support for studying the principal frequency band and critical region related to WM.Methods 32-channel EEGs were recorded from 16 healthy subjects while they perform visual WM task.The data came from 240 trials and the original EEGs were preprocessed.Fourier transform was applied to calculate spatial distributions of EEGs power in the δ,θ,α,β,γfrequency bands and the percentage of each band in the total power.Results The percentages of δ,θ,α,β,γbands in the total power were (22.44±0.86)％,(31.88±0.55)％,(24.66±1.43)％,(13.54±0.64)％,(7.47±0.48)％,respectively.The power in the θ band was significantly higher than those in the other bands (paired t-test,P＜0.001).The θ power was focused in the frontal midline region.Conclusions The principal frequency band related to WM was the θ band and the critical region was the frontal region.