BACKGROUND: In the investigation of promoting fracture healing, exogenous growth factor is very instable, expensive and inaptitude to extend generally, so how to promote the expression of endogenous growth factor effectively is worthy of further studies. OBJECTIVE: To study the effects of Shenggu injection(SGI) on the expression of basic fibroblast growth factor(bFGF) and vascular endothelial growth factor(VEGF) in fracture healing. DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed at the Orthopaedic laborary, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology between July 2007 and August 2008. MATERIALS: Sixty clean male SD rats aged 3 months were enrolled. The components of 1 g/L SGI were angelica sinensis, ground beetle, barrenwort, etc., provided by Hubei TCM hospital. METHODS: After the tibial bone fracture model was established, the SD rats were divided into two groups with 30 rats in each group, experimental group and control group. The experimental group was injected with SGI at the bone stumps, the other group was injected with the same volume of saline. This was carried once every 2 days with 0.2 mL once. Five SD rats of each group were executed to get bony callus at the 1st, 2nd, 3rd, 4th, 5th and 6th weeks after operation. MAIN OUTCOME MEASURES: The methods of immunohistochemistry were used to detect the protein expression level and differences of bFGF and VEGF in the callus tissue during different periods of fracture healing. RESULTS: The immunohistochemistry findings showed that the expression levels of bFGF and VEGF in the experimental group were higher than those in the control group in each period. CONCLUSION: SGI can improve the expression levels of bFGF and VEGF in callus tissue during fracture healing, which is maybe one of the mechanisms of SGI to promote the healing of fracture.

The statistical data on Traditional Chinese Medicine (TCM) has not been fully exploited,resulting in the gap between the storage and usage of the TCM statistical data resources. The study on the retrospective collection, arrangement and application of Traditional Chinese Medicine Statistical Data aims to enhance the usage of TCM statistical data, basing on the collection and arrangement of the authoritative TCM statistical data, common analysis of the TCM statistical data from the founding of the People Republic of China.A pilot TCM Statistics Information Database and a TCM Health Statistics Data Query System have been established with the computer database technology, the object-oriented programming tool (C#) and the Internet Browser-based presentation programming tool (ASP. Net), providing the initial functions of the data retrieval and the graphical presentation of the search results on TCM statistics data. This paper explores "statistical data-oriented with attributes description" as the idea to establish the statistical database of complex statistical data, with the solution of the data problems such as numerous of data, diverse of data units, lack of regularity and hard to be classified. The study realizes the building of TCM Statistics Information Database and the retrieval finction of TCM statistical data, which lays a foundation on the building of Traditional Chinese Medicine Industry Statistics Database and the information services of TCM statistics data in the future.

Case material search system is mainly used in the rapid search,tracing and location of field medical cases including all material in it.It can timely determine the location and volume of medical material when they are in transportation,airdrop and mobile outspread so as to improve the rescue support ability for the wounded.

The disequilibrium of histone acetylation and deacetylation may cause tumor.Histone deacetylases (HDACs) maintain the equilibrium between histone acetylation and deacetylation by catalyzing the deacetylation of histone.They are related to many regulation processes containing transcription of oncogene,cell proliferation and differentiation,apoptosis and so on.HDACs inhibitors have become the hot field of researches,more than ten different HDACs inhibitors are testing for the treatment of both hematological and solid malignancies and show obvious anti-tumor activity.

Owing to climate influence, mean daily temperature in north China differs greatly from that of the southern regions during the same season. The introduction of Fritillaria thunbergii to Beijing for cultivation was hampered by delayed emergence of seedling and a shortened period for their growth, resulting in degeneration with the underground bulb becoming smaller year after year. With forcing culture as described in this paper, the emergence of seedling came earlier and growth period prolonged. The blossom and fruit became normal.By combining the method with formalin treated seed, diseases and pest index of the bulb were reduced distinctly and the yield increased with increased proportion of larger bulb year after year.

Objective To research the applicable ways on training and examination of defibrillator utilization in grassroots units. Methods The use of the "three-step training and two-part examination" mode, that is, three-step training(theoretical instruction, grouped practice and animal experiment), a total of 83 medical officers from inferior troops of PLA were trained for the use of defibrillators, and through passing two-part test(written test and skill test), the trainees were subsequently examined so that the efficacy of the training could be evaluated. Results The trainees can be acquired satisfying achievement in the two-part test after having passed the three-step training. The respectively average scores were 86.7 ?15.9 (written test) and 90.1?14.8 (skill test). Conclusion The "three-step training and two-part test" mode is a successful defib- rillators' training mode for inferior troops.

At present,the urinalysis has many puzzles and problems,this paper is proposed consensus of the key problems including standardization method in the urine formed elements examination,the urinalysis screening test,the results of reference intervals between the different examination methods,quantitative of the visible components,using centrifugal or non-centrifugal urine to do urine formed elements examination and the development direction of automatic urine sediment analyzer.These key problems are discussed and the consensus are put forward,in order to cause concern.( Chin J Lab Med,2012,35:790-791 )

Literature of TCM contains abundant information of Chinese medicine. Traditional Chinese medicine information is characterized by its high degree of knowledge-intensive and complex inter-knowledge, so it is difficult to integrate and share knowledge. TheShanghanlun is one of the Chinese medical classics, and it includes the rules and methods of syndrome differentiation and treatment and it showed good effects in the clinical application. However, it is difficult to master for beginners. As a kind of knowledge organization tool, topic maps can flexibly define the relationship between knowledge, which realizes the visualization of knowledge network, and integrates and shares the distributed knowledge. This paper focused on the current situation of topic maps researches. The topic maps was used to organize the original content ofShanghanlun, construct the concept model of it and explore the specific application of topic maps technology in the field of traditional Chinese medicine.

Objective To explore and evaluate the changes of hemorheology and fracture healing in patients undergoing spinal operation with intraoperative autologuous blood transfusion.Methods A total of 80 patients underwent internal fixation of spine in our hospital from January 2012 to December 2014 were selected in this study.Digital random table method were utilized for dividing them into two groups.Autologous blood transfusion was used in group A,allogeneic blood transfusion was applied in group B.Blood viscosity,plasma viscosity,hematocrit,fibrinogen,erythrocyte sedimentation rate (ESR) and the level of sICAM-1,sVCAM-1 were collected and compared 1,4,8,12 weeks after the operation.Fesults Concentrated red cell and plasma volume infusion in group A was lower than that in group B,the level of sICAM-1,sVCAM-1 1,4,8,12 weeks after operation in group A were significantly lower than that in group B.There was no delayed fracture healing patients in group A,but there was 3 patients with delayed fracture healing in group B (P＜0.05).Blood viscosity,plasma viscosity,hematocrit,fibrinogen,erythrocyte and ESR 1,4,8,12 weeks after the operation compared in both group were not statistically significant (P＞0.05).Conclusion Autologous blood transfusion is not only a safe and effective method for blood transfusion,but also can improve the tissue blood supply,reduce inflammation,and conducive to fracture healing.

Objective To investigate the CT angiography techniques of the pulmonary artery and aorta at a time.Methods Twenty-three patients who underwent the 64-slice spiral CT examinations of pulmonary and aortic angiography at a time were analyzed retrospec-tively (Group A).Also,13 cases whose pulmonary angiography showed normal (Group B)and 13 cases whose aortic angiography showed normal (Group C)enrolled in this study as control groups.The contrast media dose and the radiation dose were analyzed among three groups.The abnormal blood vessels were observed,and the CT values of the normal pulmonary artery and aorta and the number of the pulmonary 4,5 grade were compared among all groups.Results The contrast media dose in Group A was more than that in Group B or Group C,but less than the sum of that in Group B and Group C.The radiation dose in Group A was equivalent to that in Group C,which was obviously less than the sum of that in Group B and Group C.Six of 23 cases were accurately diagnosed as pul-monary embolism,1 case as pulmonary malformation,4 cases as aortic dissection whose true and false lumens clearly showed with contrast,5 cases as aortitis,and 10 cases as aortic atherosclerosis.For the cases whose angiography showed normal,there was no statistically significance of the number of the 4,5 grade of pulmonary artery and the CT values of pulmonary artery and the aorta compared with those of the control group (P >0.05 ).Conclusion The 64-slice spiral CT can be used to complete pulmonary and aortic angiography at a time,and the contrast media dose and the radiation dose can be reduced obviously.