The statistical data on Traditional Chinese Medicine (TCM) has not been fully exploited,resulting in the gap between the storage and usage of the TCM statistical data resources. The study on the retrospective collection, arrangement and application of Traditional Chinese Medicine Statistical Data aims to enhance the usage of TCM statistical data, basing on the collection and arrangement of the authoritative TCM statistical data, common analysis of the TCM statistical data from the founding of the People Republic of China.A pilot TCM Statistics Information Database and a TCM Health Statistics Data Query System have been established with the computer database technology, the object-oriented programming tool (C#) and the Internet Browser-based presentation programming tool (ASP. Net), providing the initial functions of the data retrieval and the graphical presentation of the search results on TCM statistics data. This paper explores "statistical data-oriented with attributes description" as the idea to establish the statistical database of complex statistical data, with the solution of the data problems such as numerous of data, diverse of data units, lack of regularity and hard to be classified. The study realizes the building of TCM Statistics Information Database and the retrieval finction of TCM statistical data, which lays a foundation on the building of Traditional Chinese Medicine Industry Statistics Database and the information services of TCM statistics data in the future.

BACKGROUND: In the investigation of promoting fracture healing, exogenous growth factor is very instable, expensive and inaptitude to extend generally, so how to promote the expression of endogenous growth factor effectively is worthy of further studies. OBJECTIVE: To study the effects of Shenggu injection(SGI) on the expression of basic fibroblast growth factor(bFGF) and vascular endothelial growth factor(VEGF) in fracture healing. DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed at the Orthopaedic laborary, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology between July 2007 and August 2008. MATERIALS: Sixty clean male SD rats aged 3 months were enrolled. The components of 1 g/L SGI were angelica sinensis, ground beetle, barrenwort, etc., provided by Hubei TCM hospital. METHODS: After the tibial bone fracture model was established, the SD rats were divided into two groups with 30 rats in each group, experimental group and control group. The experimental group was injected with SGI at the bone stumps, the other group was injected with the same volume of saline. This was carried once every 2 days with 0.2 mL once. Five SD rats of each group were executed to get bony callus at the 1st, 2nd, 3rd, 4th, 5th and 6th weeks after operation. MAIN OUTCOME MEASURES: The methods of immunohistochemistry were used to detect the protein expression level and differences of bFGF and VEGF in the callus tissue during different periods of fracture healing. RESULTS: The immunohistochemistry findings showed that the expression levels of bFGF and VEGF in the experimental group were higher than those in the control group in each period. CONCLUSION: SGI can improve the expression levels of bFGF and VEGF in callus tissue during fracture healing, which is maybe one of the mechanisms of SGI to promote the healing of fracture.

Case material search system is mainly used in the rapid search,tracing and location of field medical cases including all material in it.It can timely determine the location and volume of medical material when they are in transportation,airdrop and mobile outspread so as to improve the rescue support ability for the wounded.

Objective To research the applicable ways on training and examination of defibrillator utilization in grassroots units. Methods The use of the "three-step training and two-part examination" mode, that is, three-step training(theoretical instruction, grouped practice and animal experiment), a total of 83 medical officers from inferior troops of PLA were trained for the use of defibrillators, and through passing two-part test(written test and skill test), the trainees were subsequently examined so that the efficacy of the training could be evaluated. Results The trainees can be acquired satisfying achievement in the two-part test after having passed the three-step training. The respectively average scores were 86.7 ?15.9 (written test) and 90.1?14.8 (skill test). Conclusion The "three-step training and two-part test" mode is a successful defib- rillators' training mode for inferior troops.

Owing to climate influence, mean daily temperature in north China differs greatly from that of the southern regions during the same season. The introduction of Fritillaria thunbergii to Beijing for cultivation was hampered by delayed emergence of seedling and a shortened period for their growth, resulting in degeneration with the underground bulb becoming smaller year after year. With forcing culture as described in this paper, the emergence of seedling came earlier and growth period prolonged. The blossom and fruit became normal.By combining the method with formalin treated seed, diseases and pest index of the bulb were reduced distinctly and the yield increased with increased proportion of larger bulb year after year.

The disequilibrium of histone acetylation and deacetylation may cause tumor.Histone deacetylases (HDACs) maintain the equilibrium between histone acetylation and deacetylation by catalyzing the deacetylation of histone.They are related to many regulation processes containing transcription of oncogene,cell proliferation and differentiation,apoptosis and so on.HDACs inhibitors have become the hot field of researches,more than ten different HDACs inhibitors are testing for the treatment of both hematological and solid malignancies and show obvious anti-tumor activity.

At present,the urinalysis has many puzzles and problems,this paper is proposed consensus of the key problems including standardization method in the urine formed elements examination,the urinalysis screening test,the results of reference intervals between the different examination methods,quantitative of the visible components,using centrifugal or non-centrifugal urine to do urine formed elements examination and the development direction of automatic urine sediment analyzer.These key problems are discussed and the consensus are put forward,in order to cause concern.( Chin J Lab Med,2012,35:790-791 )

Basing on the introductions to the three levels about the health statistics,including the concept of staffstics,the overview of the health statistics development and the current status of the Health Statistics on Traditional Chinese Medicine,we discuss the significance of Health Statistics on Traditional Chinese Medicine and analysis its existing problems,possible reasons and solutions.

Objective To evaluate the efficiency of PCR-restriction fragment length polymorphism (PCR-RFLP)aiming at the structure gene g6341,versus PCR fingerprinting analysis in the genotyping of Cryptococcus neoformans MethodsEight reference strains and 68 clinical and environmental isolates of C.neoformans were genotyped by PCR-RFLP and PCR fingerprinfing.In PCR fingerprinting,the minisatel lite-specific core sequence of wild-type phage M13 was used as a single primer.The structure gene g6341 was selected for PCR-RFLP analysis by sequence alignments of multiple genes,a pair of pnmers were developed based on the conserved region of g6341 gene.PCR products were digested with the appropriate restriction endonucleases,and RFLP profiles were analyzed.Partial sequence analysis of g6341 gene was performed for different genotypes of C.Neoformans.Phylogenetic analysis was done to study the relatedness between these genotypes.Results As sequence homology analysis showed,g6341 gene was suitable for RFLP analysis.In the case of enotyping of 76 C. Neoformans strains,the results obtained from PCR-RFLP were consistent with those from PCR fingerprinting.Sequence analysis of g6341 gene revealed a homology of 84%-97%among the eight genotypes as well as a consistency of 99%-100%within a same genotype.In the phylogenetic tree,genotypes VNⅠ,VNⅡ,VNⅢand VNⅣ belonged to one cluster,and genotypes VGⅠ,VGⅡ,VGⅢ and VGⅣ to another cluster.Conclusions PCR-RFLP analysis aiming at the structure gene g6341 is a useful tool to genotype C.neoformans.Sequence analysis of g6341 gene can disclose the relatedness among different molecular types of C.neoformans.

Object To investigate the process of flocculation impurity removal for the Anti-flu Nose Drops (AND). Methods Five kind of effective factors on flocculation impurity removal with the index of clarifying results were studied. Comparative tests for Shandong Yaosheng 1 + 1 (Ⅲ) clarificant, 50% alcohol precipitation, and 75% alcohol precipitation were performed with the quantitative index of gardenoside contents, the qualitative index of magnolin and gardenoside TLC chromatograms. The optimum process condition was selected out. Results Under the optimum process condition of flocculation impurity removal, gardenoside contents in the flocculation group is higher than those in 50% , 75% alcohol groups. TLC chromatograms showed that the spots of flocculation group are the same as those in the original liquid group. Conclusion Shandong Yaosheng 1 + 1 (Ⅲ) clarificant has following advantages; high reserved rate of active components, simple operation, cheap cost, stable process, which is a ideal method for the impurity removal.