Objective To observe the changes of protein tyrosine kinase (PTK) activity in scar tissue of rabbit ear induced by TGF-?_1 and to investigate the signal mechanism of TGF-?_1 in the scar proliferation. Methods TGF-?_1 (5 ng/ml) was applied on the wound and in the scar of rabbit ear. The PTK activity in the tissues from normal control, and 0, 14, 30, 45, and 60 d after wound epithelization was measured by phosphorus ( 32 P) incoporation. The scar changes were also observed. Results PTK activity in hypertrophic scar tissue [from (4.40?1.31) to (4.91?1.32)pmol?min -1 ?mg -1 ] was obviously elevated as compared with(2.87?0.96) pmol?min -1 mg -1 of normal skin ( P 0.05). Furthermore, PTK activity of hypertrophic scar tissue was obviously elevated as compared with that of normal scar tissue ( P 0.05) but could accelerate scar hypertrophy ( P

Objective To investigate the effects of hyaluronan (HA) and hyaluronidase (HAse) on the formation of hypertrophic scar in rabbit ear after injury. Methods Wound healing models were established on 18 adult rabbit ears, which were randomly divided into the HA treated group (Group A), the HAse treated group (Group B), the PBS treated group (Group C). The process of wound healing was observed grossly and histologically. The variation of the contents of hydroxyproline(HPr) at the stage of wound healing and post healing was measured. The relative height of scars were measured under microscope at the peaks of scar formation. Results ①The mean values of wound healing time of Group A, B and C was 11.7?0.6, 9.8?0.9, 10.8?1.0 days respectively. ②Compared with PBS controls and HAse treated group, the collagon fiber was slender and arranged in normal order in HA treated wound at early stage of wound healing. But there were no difference among them at the stage of scar formation. ③The contents of HPr were statistically lower in HA treated group than in controls at the stage of wound healing, but no difference at the stage of scar formation.The HPr contents in HAse treated group were higher than the others all the way. ④ The mean values of relative thickness of Group A, B and C were 1.55?0.35,1.89?0.39,1.59?0.29 respectively. Conclusion ①Exogenous HA on the wound surface can not affect the scar tissue proliferation;② HAse promotes the proliferation.

To study the clinicopathological and immunohistochemical characteristics of omental and mesenteric stromal tumor, 8 cases of stromal tumor were investigated, and followed up. The results showed that these tumors were solitary nodule, most were large. Histologically, cytoplasmic eosinophilia and perinuclear vacuolization were often seen in omental and mesenteric stromal tumor of spindle cell type. 3 cases showed epithelioid cytologic features. An immunohistochemical study showed that all the patients were C kit positive (100%),7 were CD34 positive, whereas 6 were SMA positive, positive expression of p53 and PCNA was found in 2 cases of malignant stromal tumor, Lower expression of PCNA was observed in 1 case of benign one.The results suggested that omental and mesenteric stromal tumors have distinct clinicopathological features, the expression of C kit and CD34 plays an important role in its diagnosis.

Objective To provide anatomical basis of the zygomatic branch of the facial nerve for the rhytidectomy. Methods The clinical surgical anatomy of the zygomatic region was studied bilaterally on 12 embalmed cadaveric head specimens and 2 fresh cadavers. Results The zygomatic branches came out of the parotid and divided into 2 or 5 branches (66.70 %), three branches (25.00 %), or multiple branches (8.80 %). 91.67 % of the rami superficialis was observed, and its branches had three types. The path of the rami superficialis could be located beforehand in a plane section. The rami superficialis and the rami profundus had various anastomosis. Conclusions Zygomatic branches divide into the rami superficialis and the rami profundus according to crossing the zygomatic majar musle. The rami superficialis distribute on the upper one third of the zygomatic majar musle, and it accounts for 91.67% in the group. It can be labeled before rhytidectomy to avoid the nerve injury.

Objective To study the effect of dimethylaminoethano (DMAE) and compound amino acid injection (AA) by mesotherapy on collagen Ⅰ and collagen Ⅲ mRNA expression levels of D-galactose-induced chemical skin aging rats.Methods 80 rats were randomly divided into individual experimental group.At 18 days after D-gal induction,rats of aging treatment groups were treated with intradermal microinjection of 0.2％ DMAE+AA,0.1％ DMAE+AA,0.2％ DMAE,0.1％ DMAE,AA,and saline,respectively,once a week for 4 weeks.At 42 days after treatment,the skin wounds were harvested.HE,PCNA,hydroxyproline and collagen Ⅰ and collagen Ⅲ mRNA expression levels of every group skins were detected.Results Dermal thickness,hydroxyproline content and collagen type Ⅰ and Ⅲ mRNA expression levels of 0.1％ DMAE+ AA and 0.2 ％ DMAE+ AA groups were significantly improved (P＜0.05),but PCNA expression of sham control group was significantly higher than all of aging groups (P＞0.05).Conclusions Local co-injection of DMAE and compound AA directly into targeted tissue under mesotherapy has marked anti-aging effects on D-galactose induced skin aging model of rat by increasing the dermal thickness,collagen content and acceleration of collagen synthesis.

Objective To explore the baggy eyelids anaplasty through posterior orbital septum fat displacement. Methods Orbital septum and posterior orbital septum fat were exposed through palpebral margin incision, internal orbital septum fat was released and sutured with arcuate expansion of capsulopalpebral fascia at the middle lower eyelid, which was taken as the function of tighting the flaccid lower eyelid at transverse direction. On such a basis, the muscle and skin were repaired and so did orbital septum and baggy eyelids.Results Ofthe 38 patients with this operation, baggy eyelids anaplasty was performed with satisfactory results.Conclusion The method of baggy eyelids plasty through posterior orbital septum fat displacement could reduce hemorrhage, prevent enophthalmos and decrease baggy eye palindromia, with better long-term effects.

Objective To investigate the distribution range and depth of the apocrine sweat glands of the axillary fossa,in order to supply with anatomic and histopathologic basis in the treatment on axillarv osmidrosis.Methods From December 2008 to ()ctober 2010,2 biopsy samples(with axillary osmidrosis),8 biopsy samples(normal,without axillary osmidrosis),were employed into the axillarv anatomy study. 25 patients with severe axillary osmidrosis were observed both maerographicallv and microscopically by using of operation and histopathological methods.Results Secretory portion of apocrine sweat glands was seen clearly,it was pitchy millet-like granules on axillary osmidrosis corpse,and pink millet-like granules in vivo.Secretory portions distributed most within the armpit hair area,exceeded the edge of armpit hair line,but not surpassed the edge of armpit hair line 1.0 cm.The depth of the apocrine sweat glands located vertically at superficial fat tissues between the dermal reticular 1aver and superficial fascia layers which were dissected away easily.Trimming with scissors under dermaIlayer,the secretory portion of apocrine sweat glands was removed cleanly without harms to reticular laver of dermas.Secretory portions became ducts under reticular layer of dermas.White Drominence-like granules were proved to be the compomers of hair follicle and sebaceous glands through Dathological section.Conclusions In order to treat axillary osmidrosis effectively,the secretary portion should be removed away through cutting off the tissues between the dermal reticular layer and suDerficial fastia layers;the ducts of apocrine sweat glands should be handled with removing hair follicle under the reticular layer of dermas．0peration area should not exceed 1.0 cm off the edge.

Objective To investigate the proliferation of rat bone marrow endothelial progenitor cells (EPCs) in the culture under different concentrations of TGF-?1 in order to optimize the culture conditions. Methods EPCs harvested from bone marrow by flushing fresh rat femur and tibia were isolated by density gradient centrifugation. The isolated cells were further purified and enriched by fast adhere to fibronectin coated dish. Flow cytometry was applied to enrich the cells positive to CD34, CD133 and VEGFR-2. EPCs were expanded in M199 medium in presence of different concentrations of TGF-?1 (10-300 pg/ml). Total cell output was recorded; and the expressions of CD34, CD133, and VEGFR-2 at different cell passages were analyzed through flow cytometer. Results Fast adhere cell group showed significantly higher positive proportion of CD34, CD133, and VEGFR-2 than unsorted cells (P

Objective To explore the correlation of data obtained through three dimensional reconstruction and measurement of mandibular angle related angles and bigonial breadth.Methods Sixty cases of adult female head spiral CT data were involved in this study.By the three dimensional reconstruction,mandibular angle related angles and bigonial breadth were measured.Through SPSS13.0 software their correlation was analyzed,including among mandibular angle related angles and these with bigonial breadth.Results The related data of the mandibular angle were measured on the image of three dimensional reconstruction; the mandibular angle was (124.28-±-4.12)°,the mandibular elevated angle was (25.52±2.22)°,the valgus angle of mandibular angle was (9.35±7.85)°,the mandiblular included angle was (77.32 ± 2.34)°,and the tangent angle of mandible was (105.53 ± 1.79)°.The correlation analysis of the related angles of mandibular angle and bigonial breadth showed that there was a significant correlation between the angles.Conclusions Three dimensional reconstruction and measurement of mandibular angle related angles and their correlation analysis can provide theoretical foundation and basis for the preoperative design and effect evaluation of mandibular angle plastic surgery.

BACKGROUND:Various factors can affect the osteogenic differentiation of human adipose-derived stem cel s, and the osteoinductive factor of traditional Chinese medicine is very important for the research of human adipose-derived stem cel s. OBJECTIVE:To investagate the effects of ginsenoside Rb1 on the proliferation and osteogenic differentiation of human adipose-derived stem cel s in vitro. METHODS:The human adipose-derived stem cel s were isolated and cultured in vitro. After passaqed to the third generation, human adipose-derived stem cel s at 2×103/wel were incubated in a 96-wel plate, and treated with 200μL of 0.5, 1.0, 2.0, 4.0,6.0μmol/L ginsenoside Rb1 medium. The human adipose-derived stem cel s in the control group were treated with an equal volume of Dulbecco’s modified Eagle medium. Growth curves were examined by 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide T colorimetric assay. Alkaline phosphatase activity and osteocalcin content were detected by alkaline phosphatase kit and radio-immunity method, respectively. Calcified nodules were observed using alizarin red O staining. RESULTS AND CONCLUSION:The proliferation viability of human adipose-derived stem cel s was significantly increased after cultured with 0.5μmol/L ginsenoside Rb1. With the increasing of the concentration of ginsenoside Rb1, the mitogenic activity of the cel s was decreased. The 6.0μmol/L ginsenoside Rb1 showed a depressant effect on proliferation. Ginsenoside Rb1 could promote alkaline phosphatase activity and osteocalcin expression in human adipose-derived stem cel s and showed a dose-dependent manner. Calcified nodule formation induced by 4.6 and 6.0μmol/L ginsenoside Rb1 were better when compared with 0.5, 1.0 and 2.0μmol/L ginsenoside Rb1. Ginsenoside Rb1 can promote the proliferation of human adipose-derived stem cel s cultured in vitro in a certain concentration, and in the high concentration, the ginsenoside Rb1 can promote the osteogenic differentiation of human adipose-derived stem cel s. So ginsenoside Rb1 can be used as an osteoinductive factor.