1.Application of Immune Checkpoint Inhibitors in EGFR Mutant Advanced Non-small Cell Lung Cancer.
Yujun ZHENG ; Wei JIANG ; Jing LI ; Lulu DAI ; Dongyan CHEN ; Yanjun LI ; Lei HUANG ; Mingji WANG
Chinese Journal of Lung Cancer 2022;25(9):671-677
In recent years, immune checkpoint inhibitors (ICIs) have greatly improved the survival rate of non-small cell lung cancer (NSCLC) patients without driver mutation. Compared with wild-type tumors, tumors with epidermal growth factor receptor (EGFR) mutations have greater heterogeneity in immune microenvironment characteristics such as programmed cell death ligand 1 (PD-L1) and tumor mutational burden (TMB). Whether ICIs is suitable for NSCLC patients with EGFR mutation has been controversial. Clinical studies have shown that immunomonotherapy has no significant effect on patients with EGFR mutant NSCLC. ICIs combined with chemotherapy and antiangiogenic drugs show good survival benefits. This paper overviews the clinical research and related mechanism of ICIs single drug or combination therapy inadvanced NSCLC patients with EGFR mutation.
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B7-H1 Antigen/metabolism*
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Carcinoma, Non-Small-Cell Lung/pathology*
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ErbB Receptors/metabolism*
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Humans
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Immune Checkpoint Inhibitors/therapeutic use*
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Ligands
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Lung Neoplasms/pathology*
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Mutation
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Tumor Microenvironment
2.Effect of growth hormone releasing hormone on chondrocytes of osteoarthritis
The Korean Journal of Internal Medicine 2022;37(1):222-229
Background/Aims:
To evaluate the effect and possible mechanism of growth hormone releasing hormone (GHRH) on chondrocytes of osteoarthritis (OA).
Methods:
Articular chondrocytes were cultured and the expression of GHRH receptor in chondrocytes was detected. Then recombinant adenovirus GHRH (Ad- GHRH) was transfected to one group of chondrocytes. The expression of collagen type II, matrix metalloproteinase 13 (MMP-13) and signal transducer and activator of transcription 3 (STAT3) in each experimental group was determined by Western blot.
Results:
The GHRH receptor was expressed in chondrocytes, and this provided a basis for further study of the role of GHRH in chondrocytes. Cell proliferation of the Ad-GHRH group was significantly higher than that of the OA group by CCK-8 assay. Compared with the OA-group, the protein expression of MMP‑13 was decreased in the Ad-GHRH group. Compared with the OA-group, the protein expression of collagen type II, phosphorylated STAT3 (P-STAT3) were increased in the Ad-GHRH group.
Conclusions
Our results show that the GHRH receptor is expressed in chondrocytes. GHRH can promote the proliferation of chondrocytes and the synthesis of type II collagen, and increase the extracellular matrix, which is achieved by phosphorylated STAT3 protein.
3. Anti-lymphoma effect of arsenic preparations:research advances
Journal of International Pharmaceutical Research 2020;47(4):258-262
Arsenic was originally regarded as a toxic substance. For example, arsenic trioxide is highly toxic. But in recent years, arsenic compounds have played an important role in cancer treatment. Many studies have shown that arsenic preparations have a wide range of anti-tumor effects, especially with the significant effect seen in the treatment of leukemia. However, there have been few studies on the therapeutic effect of arsenic preparations on lymphoma so far. This paper reviews the research progress in the antilymphoma effect and related action mechanism of arsenic preparations, so as to provide a reference for further studies on the lymphoma treatment with arsenic preparations.
4.N1 characteristics and influencing factors in non-small cell lung cancer of T2 stage
LI Shanshan ; XU Ning ; GAO Yan ; KONG Qinglong ; CHANG Xu ; LI Jun
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery 2018;25(4):293-298
Objective To investigate the characteristics and influencing factors of N1 in T2 stage of the 8th TNM stage of The International Association for the Study of Lung Cancer (IASLC) (3 cm
5.Evaluation for Parkinson's Disease with Chinese Version of MDS-UPDRS and Original UPDRS
Yan QU ; Xiao-hong LI ; Ya-nan SUN ; Ya-hui LI
Chinese Journal of Rehabilitation Theory and Practice 2019;25(8):936-939
Objective:To evaluate the reliability and validity of Chinese version of International Parkinson and Movement Disorder Society (MDS)-Unified Parkinson's Disease Rating Scale (UPDRS), and then do comparative analysis with original UPDRS. Methods:From May, 2016 to December, 2018, 225 patients with Parkinson's disease were respectively evaluated by MDS-UPDRS and UPDRS, and then the reliability and validity of MDS-UPDRS were evaluated. Correlation between two scales was analyzed. Results:The Cronbach's coefficient of the total MDS-UPDRS was 0.734. Rank correlation's coefficient was 0.905. For factor analysis, KMO was 0.728. Pearson's correlation analysis showed that the correlation's coefficient of the common factor of the two scales was 0.969, while the correlation's coefficient of the total score of the two scales was 0.972. Compared with UPDRS, MDS-UPDRS added a number of non-motor symptoms, which adjusted the standard of measurement and increased the accuracy degree of evaluation at early stage of disease. Besides, MDS-UPDRS added a lot of instructions to evaluate the standards to make the evaluation normatively. Conclusion:Chinese version of MDS-UPDRS has high reliability and validity, and highly correlate with the original UPDRS. It is more suitable for Chinese patients with Parkinson's disease in clinical evaluation than UPDRS.
6.Preparation of cryoprecipitate coagulation factor from liquid-state plasma by supercooling method
Wei KANG ; Delong LI ; Dong WANG ; Manhua ZHOU ; Juanjuan LI
Chinese Journal of Blood Transfusion 2021;34(11):1261-1262
【Objective】 To establish a novel preparation method of cryoprecipitate coagulation factor from overcooled liquid-state plasma. 【Methods】 The fresh liquid plasma was kept at -11℃ to -13℃ for a period of time. It can remain in the liquid state with some coagulation factors generated due to supercooling. Then cryoprecipitate can be obtained from the liquid plasma by siphon method. 【Results】 The average fibrinogen content yielded in cryoprecipitate, prepared from 50 samples of 16-hour-stored fresh liquid plasma, was (186.02±22.72) mg, with the average recovery rate of (37.51±7.42) %, and the average content of coagulation FⅧ was (104.66±22.88) IU, with the average recovery rate of (46.62±5.58) %. 【Conclusion】 The cryoprecipitate coagulation factors could be obtained not only from fresh frozen-thawed plasma, but also from overcooled liquid plasma which is simple and stable, also meets the requirements of relative standards.
7. Types of nursing research papers and attentions before contributions
Chinese Journal of Practical Nursing 2019;35(36):2879-2881
Before writing nursing research papers, the authors need to determine the type of papers besides the topic planning. Different types of papers have different requirements for scientific research design, statistical methods, organization and collection of materials. Format specification is the basic requirement of paper writing. It is also the knocking brick for the paper to pass the preliminary examination. At the same time, mastering the attentions before contributions can increase the probability of paper recruitment. This article briefly introduces the types of nursing scientific research papers and the attentions before contributions, with a view to providing guidance for the author′s contributions.
8.Effects of Genipin on the expression of uncoupling protein 1 in brown adipose and white adipose tissues in mice.
Na-Na SHEN ; De-Zheng GONG ; Feng-Kai ZOU ; Yu YAN ; Li-Li GUAN ; Yuan ZOU
Chinese Journal of Applied Physiology 2019;35(1):74-78
OBJECTIVE:
To investigate the effects of genipin on promoting brown adipose tissue activation and white adipose tissue browning.
METHODS:
The male C57BL/6J mice were divided into three groups: normal control group, genipin group and cold-stimulus group.Genipin group were treated consecutively with genipin at a dose of 15 mg/kg once a day for 9 days, normal control group were treated with the saline.The mice with cold-stimulus were exposed to 4℃ environment for 5 days.Daily food amount and body weight were measured.Morphological changes were observed in the subscapular region, inguinal region and epididymis around the adipose tissue.The expression of uncoupling protein 1 (UCP1) was determined by real-time PCR and Western blot respectively.
RESULTS:
The wet weight of white fat in genipin-treated mice was decreased by 16% , and 28% in that of cold-stimulus mice, compared with the normal control group (P<0.05).After treatments of genipin and cold-stimulus, the color of white adipose tissues was darker, and the size of lipid droplets in adipocytes was smaller, whereas the number was increased.Compared with the normal control group, UCP1 expression was increased obviously in fat tissues, including the subcutaneous and visceral white adipose tissues, and brown adipose tissue after treated with genipin and cold-stimulus (P<0.05).
CONCLUSION
Genipin promoted activation of brown adipose tissue and browning of white adipose tissue by upregulating UCP1 expression, which could contribute to the loss of body weight against obesity.
Adipose Tissue, Brown
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drug effects
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Adipose Tissue, White
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drug effects
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Animals
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Cholagogues and Choleretics
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pharmacology
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Iridoids
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pharmacology
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Male
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Mice
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Mice, Inbred C57BL
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Obesity
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drug therapy
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Uncoupling Protein 1
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drug effects
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Up-Regulation
9.Research Progress on Exosome in Malignant Tumors.
Yujun ZHENG ; Wei JIANG ; Dongyan CHEN ; Lei WANG ; Yanjun LI ; Lulu DAI ; Lei HUANG ; Mingji WANG
Chinese Journal of Lung Cancer 2020;23(8):689-694
"Liquid biopsy" have been intensively studied in recent decades. The exosomes carry their maternal partial functional proteins and genes. As a form of intercellular vesicular transport, exosome-mediated intercellular communication participates in a variety of physiological and pathological processes. The information transmission mediated by exosomes is associated with pathophysiological status and plays an important role in the pathogenesis of cancer. Their detection may be beneficial for the diagnosis and treatment of tumor. This article reviews advances in knowledge on the exosome properties, the effect of exosomes on malignant tumorigenesis, and their clinical application. The data will provide a reference for clinical practitioners.
10.Serological and molecular characterization of HBV infection with HBsAg reactive and HBV DNA non-reactive
Chunxiang LI ; Xiaohan GUO ; Peng SUN ; Xiaochun LIU ; Dan LIU ; Lei ZHOU ; Liang ZANG ; Daniel CANDOTTI ; Xuelian DENG
Chinese Journal of Blood Transfusion 2022;35(9):950-954
【Objective】 To investigate the serological and molecular characteristics of HBsAg+ /HBV DNA non-reactive (NR) infections. 【Methods】 Samples tested as HBsAg+ and HBV DNA NR were confirmed by individual NAT repeat testing, viral particle concentration by PEG precipitation combined with in-house nested PCR and real-time quantitative PCR, anti-HBc testing, and HBsAg quantification. HBV sequences were compared with those from donors with chronic and occult infection as controls. 【Results】 A total of 792 195 samples were screened between January 2011 and December 2020, of which 53 (1: 14 947) were confirmed HBsAg+ /HBV DNA NR. HBV DNA was detected further in five (9.4%) samples; three S sequences and four Pre Core/Core sequences were obtained. Unique amino acid substitutions (P130T, P135Q/S, R151Q, G153S and S155F) were found in the Core protein that may affect virus packaging and replication. 【Conclusion】 Extremely low HBV DNA level was detected in plasmas of HBsAg+ /HBV DNA NR donors. Barely detectable HBV DNA might be associated with unusual mutations in the Pre Core/Core protein affecting viral replication. More sensitive HBV DNA and/or HBsAg assays may be considered to further reduce the potential HBV transfusion-transmission residual risk.