Objective To investigate the clinical effect of chronic dacryocystitis through dacryocystorhinostomy.Methods The clinical data about 104 eyes in 84 chronic dacryocystitis patients who treated by dacryocystorhinostomy were analyzed.Than summarized and analysed the preoperative preparation,operation method and the curative effect after operation.Results The 104 eyes operation were all successful.The patients followed up for 6 to 24 months.96 eyes had no symptom of tear overflows and overflow pus(approximately 92.31%),2 eyes recurrenced after 1 year,then after operation recovered again(approximately 1.92%),4 eyes had symptom of tear overflows,but tear road washing unobstructed (approximately 3.85%),2 eyes lost to follow up(the rate Was about 1.92%).Conclusion The clinical treatment on the chronic dacryocystltis by dacryocystorhinostomy was effective and the recurrence rate wag very seldom.

Two kinds of different functional groups modified RuBpy-doped silica fluorescent nanoprobes Probe A and B that conjugated with avidin were prepared for the recognition of liver cancer cells. Firstly RuBpy-doped silica nanoparticles were synthesized by reverse microemulsion and modified with different functional groups, then Probe A was prepared by the conjugation of avidin with carboxyl modified nanoparticles through covalent binding using 1-ethyl-3-( 3-dimethylamino propyl ) carbodiimide hydrochloride ( EDC )/sulfo-NHS, whereas Probe B was prepared by the conjugation of avidin with the polyethylene glycol ( PEG) linkers on the surface nanoparticles using cyanogen bromide method. Therefore, compared with Probe A, Probe B was obtained by coupling avidin to the nanoparticles through long-chain PEG molecules. The two probes were incubated with liver cancer cells respectively, and microscopic fluorescence imaging shows that Probe B which contained PEG molecules could be more effectively applied for the recognition of tumor marker carcinoembryonic antigen ( CEA) in liver cancer cells.