Objective To investigate the immune effect of neonatal hepatitis B vaccination and its once more immune effect in low or no responders .Methods Screening Songzi city MCH 2 000 cases of hepatitis B vaccine 7-12 months baby's first immunization effect ,analyze the impact of the prime factors for early response in children with low or no free choice 10μg Hansenula yeast recombinant hepatitis B vaccine or 5μg hepatitis B vaccine ( HepB-SC) , carried revaccination(Immune again),then analyzed the effect of free 1,3 times.Results (1) Primary immune response rate of neonatal hepatitis B vaccine in 2 000 cases was 92.2%,the average anti-HBsGMC was (321.88 ± 100.45) IU/L,in which the HBsAg positive persons and birth in the township hospital neonatal response rate was low that had a statistically significant (χ2 =30.61,47.58,all P <0.05) difference with the children whose parents'HBsAg was negative and borned in hospitals above the county level;( 2 ) GMC content was increase in once more immune at 1,3 times than before,the difference was statistically significant (t=98.32,102.15,all P<0.05),and the 3 time is higher than the 1 time once more immuneof levels of GMC ,the difference was statistically significant ( t=98.55,102.33,all P<0.05),content of GMC is higher in once more immune of Hansenula hepatitis B vaccine at 1, 3 times when compared with the recombinant hepatitis B vaccine , the difference was statistically significant ( t =104.11,124.31,all P<0.05).Conclusion Low and non-responders'proportion is high in the children whose par-ents'HBsAg is positive and be born in the primary hospital ,which has a higher response rate after once more immune , Hansenula hepatitis B vaccine has a better immune effect .