1.A study on the comparability of SCID-Ⅱ and PDQ-4
Chinese Journal of Behavioral Medicine and Brain Science 2008;17(5):468-469
Objective To study the comparability of two personality diagnostic instruments(SCID-Ⅱ and PDQ-4)in psychiatric patients. Methods One hundred and twelve mental disorder patients were investigated with the SCID-Ⅱ and PDQ-4. Results 1)Significant differences were found between groups(dividing by total scores on PDQ-4)by means of SCID-Ⅱ interviews(P<0. 0 1). 2)Categorical personality disorder(PD)groups by means of SCID-Ⅱ interviews had hisher scores on PDQ-4 than their related non-PD groups. 3)For agreement on categorical diagnoses between SCID-Ⅱ and PDQ-4, the correlation coefficients varied from 0. 17 to 0. 57. Except for antisocial PD(r=0. 57), the others had poor-fair coefficients, as r<0. 50. Conclusions In general, there is some correlation between SCID-Ⅱ and PDQ-4. Low agreement between PDQ-4 and SCID-Ⅱ is observed for categorical PD evaluations. Thus, PDQ-4 can't be a substitute tool for SCID-Ⅱ.
2. Systemic toxicity of (S)-(-)-pantoprazole sodium in rats following intravenous injection for 30 d
Chinese Pharmaceutical Journal 2013;48(13):1060-1064
OBJECTIVE: The systemic toxicities of S-(-)-pantoprazole sodium in rats following intravenous injuction for 30 d were studied. METHODS: One hundred rats were divided randomly into 5 groups: vehicle control group, pantoprazole sodium control group and three S-(-)-pantoprazole sodium groups with different dosages, and received vehicle, pantoprazole sodium(80 mg·kg-1·d-1), S-(-)-pantoprazole sodium(80, 40 and 20 mg·kg-1·d-1) by i.v.via tail vein. Administrations were performed each day for consecutive 30 d. Haematological parameters, biochemical parameters, and histopathology analysis were determined at 30 d of treatments and 14 d after the withdrawal, respectively. RESULTS: The rats in the S-(-)-pantoprazole sodium group at 80 mg·kg-1·d-1 apperanced shortness of breath, unsteady gait, lying motionless and other symptoms. The levels of TC, Na+, Cl- in this group were significantly higher or lower than those in vehicle control group at 30 d(P<0.05), The change of these parameters regained to normal at 14 d after withdrawal. CONCLUSION: Intravenous administration of the S-(-)-pantoprazole sodium for 30 d at high dose could induce reversible damage to liver and electrolyte. The toxicity of S-(-)-pantoprazole sodium is similar with pantoprazole sodium at the same dosage.
3.Determination for Total Flavonoids in Health Food with the Ultrasonic Extraction Technology
Jian WU ; Xingbi DAI ; Xiao XIAO
Journal of Environment and Health 1992;0(04):-
extraction time.The extraction of total flavonoids in health food was the highest under the situations of ethanol concentration 60%,solid-to-liquid ratio 1:20 and ultrasonic time 20 min.The average value was 5.370 mg.The linear range of total flavonoids was between 1.0-40 ?g/ml while the regression equation is y=0.003 3x-0.010 9,correlation coefficient was 0.999 5 and detection limit was 1.0 ?g/ml.The standard addition recovery rates of this method were 95%-104%(n=3),RSD was 1.717%(n=5).Conclusion Under the optimum situation,this method has high precision and accuracy while the blank is low and stable as well.It is applicable to the detection of total flavonoids in health food and some other foods.
5.Role of cAMP-PKA signal transduction pathway in lidocaine-induced up-regulation of expression of surfactant protein-A in rat alveolar epithelial type Ⅱ cells
Changzong DAI ; Deming WANG ; Ji XIAO
Chinese Journal of Anesthesiology 2014;34(5):620-623
Objective To evaluate the role of cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) signal transduction pathway in lidocaine-induced up-regulation of the expression of surfactant protein-A (SP-A) in rat alveolar epithelial type Ⅱ cells (AEC Ⅱ).Methods Healthy male Sprague-Dawley rats were sacrificed and AEC Ⅱ were isolated,purified and incubated in 24-well culture plates (100μd/hole) with density of 1 × 106/ml.After being incubated for 2 h,the culture medium was replaced with serum-free medium DMEM.The cells were randomly divided into 4 groups (n =48 each):control group (group C),forskolin (adenylate cyclase agonist) group (group F),lidocaine 200 μg/ml group (group L),and PKA inhibitor H89 + L group (group P+ L).Forskolin 10 μmol/ml was added to DMEM in group F.Lidocaine 200 μg/ml was added to DMEM in group L.H89 10μnol/ ml was added to DMEM and AEC Ⅱ were incubated for 10 min,and then lidocaine 200 μg/ml was added in group P + L.At 6,12 and 24 h of incubation (T1-3),cAMP content and PKA activity (using ELISA),and expression of SP-A mRNA (by real-time fluorescent quantitative PCR) and SP-A (by Western blot) were measured.Results Compared with group C,the expression of SP-A mRNA and SP-A was significantly upregulated,and cAMP level and PKA activity were increased at T1-3 in group F and at T2,3 in group L.Compared with group L,the expression of SP-A and SP-A mRNA was down-regulated,PKA activity was decreased,and no significant change was found in cAMP level at T1-3 in group P + L.Conclusion Lidocaine can up-regulate the expression of SP-A in AEC Ⅱ of rats through activating cAMP-PKA signal transduction pathway.
6.Effect of torasemide or furosemide combined with mannitol in treatment of acute cerebral hemorrhage patients with cerebral edema
Yanbei YANG ; Jingcun DAI ; Xiao DONG
Chinese Journal of Postgraduates of Medicine 2014;37(6):42-44
Objective To compare the effect of torasemide or furosemide combined with mannitol in the treatment of acute cerebral hemorrhage patients with cerebral edema.Methods One hundred and sixty patients with cerebral hemorrhage were selected,and divided into torasemide group and furosemide group according to different treatment methods,40 cases in each.The torasemide group was treated with torasemide combined with 20% mannitol,the furosemide group was treated with furosemide combined with 20% mannitol.The curative effect,mannitol dosage,edema volume,24 h urine and adverse reactions in two groups were compared.Results The total effective in torasemide group was higher than that in furosemide group [97.5% (78/80) vs.77.5% (62/80)],mannitol dosage was less than that in furesemide group [(347.5 ±32.5) ml vs.(438.2 ±30.7) ml],the incidence of adverse reactions was lower than that in furosemide group [7.5%(6/80) vs.27.5%(22/80)],which reached statistical significance (P< 0.01 or < 0.05).The edema volume in the 7th,14th day in torasemide group was less than those in furosemide group [(21.1 ±3.4) ml vs.(23.3 ±4.8) ml,(17.6 ±4.5) ml vs.(22.4 ±5.6) ml],the 24 h urine in the 3re,7th,14th day were more than those in furosemide group [(3 684 ±528) ml vs.(3 429 ±592) ml,(3 854 ± 746) ml vs.(3 185 ±490) ml,(3 742 ±t658) ml vs.(2 251 ± 324) ml],which reached statistical significance (P < 0.05).Conclusions Torasemide in treatment of acute cerebral hemorrhage patients has better efficacy and safety.It is better than furosemide.
7.Molluscicidal effect of 5%niclosamide ethanolamine granules against Onco-melania hupensis in a marshland field
Rongfeng WU ; Min XIAO ; Jianrong DAI
Chinese Journal of Schistosomiasis Control 2014;(5):573-574
Objective To investigate the molluscicidal effect of 5%niclosamide ethanolamine granules against Oncomela-nia hupensis in a marshland field. Methods The 5%niclosamide ethanolamine granules were sprayed at a dose of 40 g/m2 on 3 snail-breeding marshlands in Yangzhong City of Jiangsu Province to assess its field molluscicidal actions while 26%suspension concentrate of metaldehyde and niclosamide MNSC) at a dose of 4 g/m2 and fresh water served as controls. Results After seven days spraying, 5%niclosamide ethanolamine granules resulted in a of snail mortal85.42%ity while the mortality rates of snails were 82.35% and 2.86% in the MNSC and water control groups respectively. Conclusion 5% niclosamide ethanol-amine granules exhibit a high molluscicidal activity which is suitable to be used in the mashland.
8.Effect of implanted sustain-released 5-fluorouracil during operation in patients with advanced rectal cancer
Ling DAI ; Xiaolin TIAN ; Xiao CHEN
Clinical Medicine of China 2011;27(4):430-433
Objective To assess the effect of intraperitoneal interstitial implant of Sustained-release 5-Fluorouracil during operation for advanced rectal cancer. Methods One hundred and four cases with advanced rectal cancer were enrolled into the study and divided into 3 groups: treatment group 1(n = 37), underwent combined hypotonic peritoneal chemo-hyperthermia and Sustain-released 5-fluorouracil implantation treatment during operation; treatment group 2(n = 34), underwent combined intraoperative hypotonic peritoneal chemohyperthermia and early postoperatively intraperitoneal chemotherapy treatment; and control group(n = 33),underwent peritoneal lavage during radical resection alone. Results Digestive tract toxicity in group 1 was significantly lower than that in group 2(2. 70% vs. 20. 59%, x2 = 9. 104, P < 0. 05). There were significant difference on 1 year survival rate among the three groups. The 2-year survival rate of group 1 was significantly higher than that in the control group (78. 37% vs. 48. 48%, x2 = 1. 297 ,P < 0. 05). After two years follow-up the 2-year local recurrence rate of group 1 was significantly lower than that in the control group(21.62% vs 32. 35 %, x2 = 7. 001, P < 0. 05). However, no significant difference was found compared to group 2, which was 32. 35%. Conclusion lntraperitoneal interstitial implant of Sustain-released 5-Fluorouracil during operation is an effective intraperitoneal chemotherapy with mild side effects for advanced rectal cancer.
9.Laser-induced choroidal neovascularization inhibited by tetrandrine in rats
Dai LI ; Shuiqing ZENG ; Xiao CHEN
Chinese Journal of Ocular Fundus Diseases 2000;0(04):-
Objective To investigate the effect of tetrandrine (Tet) on experimental choroidal neovascularization and the effect of Tet on retinal structure and function. Methods Choroidal neovascularization was induced in 20 Brown Norway (BN) rats (40 eyes) by diode laser (wavelength: 810 nm; exposal time: 0.1 second; facular diameter:100 ?m; energy: 120 mW), and the rats were divided randomly into experimental and control group with 10 rats (20 eyes) in each group. In experimental group, 0.05 ml Tet with the concentration of 3.21 ?mol/L was injected intravitreously 0 and 3 days after laser photocoagulation; in the control group, the rats underwent an intravitreous injection with the same volume of sodium chloride solution. The incidence of CNV was evaluated by fundus fluorescein angiography (FFA) 14 days after laser photocoagulation. Five right eyes of another Five healthy BN rats underwent intravitreous injection with 0.05 ml Tet with the concentration of 3.21 ?mol/L, and an intravitreous injection with the same volume of sodium chloride solution was performed on the left eyes. Before injection, 1 hour, and 1 day after the first injection, and 1 hour, 1 day, 7 days, 14 days after the second injection the electroretinography (ERG) was performed on these 5 rats; 14 days after the second injection, the retinae were examined by light microscopy and transmission electron microscopy. Results The incidence of CNV was 23.26% in experimental group,which was obviously lower than that in the control group (63.33%) (P0.05). There were no structural changes of retinal tissues examined by light and electron microscopy. Conclusion Tet may inhibit choroidal neovascularization in rats; there isn′t any significant toxic effect of intravitreous injection with Tet on retina at the dosage of 3.21 ?mol/L.
10.Construction of recombinant retroviral vector of short interfering RNAs specific for macrophage migration inhibitory factor (MIF) and establishment of stable HeLa cell line with a persistent knockdown of MIF
Bo DAI ; Dingzhang XIAO ; Xiyong YU
Chinese Journal of Pathophysiology 2000;0(11):-
AIM:To construct recombinant retroviral vector of short interfering RNAs (siRNA) specific for macrophage migration inhibitory factor (MIF) and to establish the stable knockdown of MIF cell line of mammalian cells by transfecting the recombinant retroviral vectors. METHODS: We synthesized oligo-nucleotides for MIF in vitro, and cloned them into retroviral vector pSuper.retro. Subsequently the plasmids were sequenced and digested to identify the construction of the recombinant retroviral vectors. The vectors RNAi were transfected into packing cell line PHOENIX, which was selected by puromycin later. HeLa cell line was infected by the virus supernatant of stable PHOENIX cell lines, and the stable HeLa cell line showed significantly to silence MIF was established by selecting with puromycin. We also compare the characters of HeLa-pSuper-mock to HeLa-pSuper-MIF cells by using migration assay, adhesion assay, soft agar assay and FACS analysis of the cell-cycle progression. RESULTS: The recombinant retroviral vectors were constructed successfully. The HeLa cell line infected by the supernatant containing the retrovirus of package PHOENIX cells was persistent knockdown of MIF confirmed by Western blotting. Knockdown of MIF in HeLa cells inhibited the migration and adhesion, and decreased the clone formation. FACS analysis revealed that knockdown of MIF arrested HeLa cells in G0/G1 phase. CONCLUSION: We establish the stable HeLa cell line with a persistent knockdown of MIF. Our current studies reveal that MIF is necessary for HeLa cell migration and anchorage-independent growth.