Objective To discuss the curative effect of Choledo-choledochostomy by observing different means of biliary duct reconstruction in the iatrogenic bile duct damage and the liver trans-plant. Method The clinical data of 19 patients who were found to have iatrogenic bile duct injury dur-ing operation and immediately repaired and 66 patients receiving liver transplantation in our hospital in the past 10 years were retrospectively analyzed. Meanwhile, the means for bile duct reconstruction were discussed. Results The rate of curative effect of the Choledo-choledochostomy was 86.6% for iatrogenie bile duct injury. However, whether to leave the T-tube or not was not a determinant factor for the procedure in liver transplantation (P>0. 05). Conclusion Choledo-choledochostomy has good curative effect for iatrogenic bile duct injury and liver transplantation. During the operation, the bile duct may not be excessively freed to guarantee blood supply in at the anastomosis site to further en-hance the curative effect.

Objective To analyze and compare thh effect of early surgical treatment and nonsurgical treatment for patients with traumatic severe acute pancreatitis.M~ethods The curative effect,surgical procedures and mortality data in 36 cases were retrospectively analyzed.ResUlts Compared with nonsurgieal treatment,early surgical treatment was associated with a significantly lower incidence of mortality,and with a highter cure rate (9.52% vs 33.33% ,P ＜ 0.05).Conclusion For traumatic severe acute pancreatitis,early operative management should be selected routinely as the first choice of treatment.

90 ). ③For the experimental group at baseline, DQ of the gross movement was the highest (71.5?10.5), while that of the other four areas were similar (fine movement 56.7?10.4, adaptability 59.6?12.5, language 54.3?11.7, social 56.8?14.7).Conclusions:Early intervention had significant effects for the development of children with mental retardation, which medicine has less role.

Object: To explore the effects and the influent agents of early education on the healthy termed infants. Methods: 52 healthy termed infants voluntary to the exercise center as experimental group were early educated. The other 52 infants as control group were in the same elementary conditions comparing to the experimental group. The experimental group was trained the corresponding projects and examined DQ at the beginning and the 3 rd,6 th,9 th month after training. The control group was only examined DQ at the beginning and after 9 months. Results:①At the 9 th month after training, DQ of the experimental group was improved 21.9?13.6, which was much 7 times higher than that of the control group, which was improved 3?3.0 (P

Fecal samples were collected from 81 patients with digestive tract bleeding (including 32 cases of upper digestive tract bleeding, 6 gastric cancer, 34 lower digestive tract ulcer and 9 colorectal cancer) and 40 healthy subjects. Fecal occult blood was detected with hemoglobin and/or transferrin monoclonal immunoassay. The positive rate with hemoglobin immunoassay for upper digestive tract bleeding, gastric cancer, lower digestive tract ulcer and colorectal cancer was 46.9%, 66.7%, 32.3% and 77.8% respectively;the positive rate with transferrin imrnunoassay was 71.9%, 83.3%, 47.1% and 88.9% respectively. When two methods were combined, the positive rate increased to 87.5%, 100%, 55.9% and 100% respectively showing a higher sensitivity.

Objective To investigate the radiological characteristics and the relationships between the parameters by X?ray and direct magnetic resonance arthrography (dMRA) in non?completely dislocated dysplasia hips. Methods We retro?spectively reviewed patients (above 13 years old) with dysplasia hips from August 2009 to August 2012. These patients were classi?fied as Hartofilakidis typeⅠand typeⅡ. 188 patients (274 hips) involved 28 males (30 hips) and 160 females (244 hips), average aged 27.3 years (range 13-47 years). Standard pelvic A?P, bilateral 65° oblique X?rays, and dMRA were conducted in each pa?tient. Lateral central?edge angle (LCE), anterior central?edge angle (ACE), femoral neck?shaft angle (FNSA), and tonnis acetabu?lar index (AI) angle were measured based on the X?ray. The dysplasia hips were classified into 4 grades (GradeⅠ:CE 11°-20° , GradeⅡ:CE 1°-10°, GradeⅢ:CE-9°-0°, and GradeⅣ:CE≤-10°). Femoral neck anteversion angle (FNA), labral tear, labral inversion, and labral cyst was observed on dMRA films. Variance analysis and rank correlation were applied to find the relation?ship between CE grades and the relevant parameters by using SPSS 19.0. Results LCE has negative correlation with AI and grade of osteoarthritis, and has positive correlation with age, ACE. However, there was no relationship between FNSA and FNA. There was significantly different in Shenton's line, labral tear, labral inversion, cartilage lesion, among different grade of LCE. Conclusion LCE is an important parameter used in evaluating for dysplasia hips without complete dislocation. LCE can reflect age of onset, ACE, AI angle and degree of joint damages. Evaluation for the severity of non?completely dislocated dysplasia hips can be conducted according to grades of CE.

Objective To investigate the ultrastructural characteristics of amelanotic melanocytes (AMMCs). Methods Individual hair follicles from normal human scalp were digested with collagenase type V, then washed in phosphate buffer saline. Hair-follicle cell suspensions were prepared by trypsin and cultured in a medium suitable for melanocyte growth. The keratinocytes were removed by differential trypsinization. Geneticin (100?g/mL) was used to eliminate contaminating fibroblasts. After 3 passages the cells were trypsinized, washed in phosphate buffer saline, and finally processed for transmission electron microscopy. Results Under transmission electron microscope, the cultured cells were round or oval-shaped with a single large nucleus and double-layered karyotheca. Abundant euchromosome but sparse heterochromosome was observed within the nucleus. There were various organelles in the cytoplasm, including mitochondria, rough endoplasmic reticulum (RER), ribosomes and abundant melanosomes of nearly uniform size. The electronic density granules distributed in a concentric pattern in most of the melanosomes. Colgi complexes were inconspicuous in the cells. Conclusions Compared to epidermal melanocytes, AMMCs from human hair follicles have different ultrastructural characteristics which implies their functional immaturity. AMMCs may serve as the depot for mature melanocytes.

Objective:To study the effects of combination of parenteral and enteral nutrition in sequence on prognosis of severe craniocerebral injury of geriatric patient.Methods:The method and effects of nutrition support have been studied in 84 cases of severe craniocerebral injury of geriatric patient.The patients of study group were fed by parenteral and enteral nutrition in sequence in early period,and the patients of the control group were nutritionally supported with common method for 6-7 days.Results:Nitrogen cumultive balances,nutitional index of the study group were signficantly better than that of the control group(P

Objective To analyze the impact of dose calculation resolution on Gamma pass rate for planar dose distribution verification of intensity-modulated radiotherapy (IMRT),and to find out the appropriate calculation resolution value with specific applied criteria.Methods 25 IMRT plans were selected for the planar dose verification.The Gamma pass rates with 3 mm/3％,2 mm/2％ and 1 mm/1％ criteria were compared and analyzed.The planar dose distributions were calculatedand exported from TPS with different resolutions of 0.5,1,2,3,4 and 5 mm.The pass rates of Gamma analysis between the computed dose distributions with different resolutions and the dose distributions measured by Mapcheck device were compared and analyzed.Results The average Gamma pass rates of all the 225 fields decreased with the increment of calculation resolution.When the pass rates of Gamma analysis were calculated using 3 mm/3％ criteria,the gamma pass rate with 1 mm and 0.5 mm calculation resolution were (98.3±1.3)％ and (98.3±1.2)％,respectively.The results were almost the same and the difference wasn't statistically significant (P＞0.05).The gamma pass rates with greater than or equal to3 mm calculation resolution were significantly less than the pass rate with 0.5 mm calculation resolution (P＜0.05).When the pass rates of Gamma analysis were calculated using 2 mm/2％ criteria,all the pass rates with greater than 0.5 mm calculation resolution were significantly less than the pass rate with 0.5 mm calculation resolution (P＜0.05).Conclusions The Gamma pass rates of planar dose verification reduce significantly with the increment of calculation resolution.In order to reduce the effect of calculation resolution on Gamma pass rate,the lower calculation resolution should be used.When using the 3 mm/3％ criteria for the Gamma analysis,1 mm calculation resolution is recommended.When using the 2 mn/2％ criteria for the Gamma analysis,0.5 mm calculation resolution is recommended.The appropriate calculation resolution will ensure the reliability of planar dose verification.

Objective To reconstitute hair follicles in mice using graft chambers,and to study the effect of different cell types on hair follicle regeneration.Methods Full-thickness skin was obtained from the back of C57BL/6 neonatal mice.Then,epidermal cell suspensions were prepared by shredding epidermis after trypsinization,hair follicles and dermal cells were collected by filtration,low-speed centrifugation and density gradient centrifugation,and hair follicle epithelial cells were obtained via trypsinization of hair follicles followed by filtration.Nude mice were classified into four groups to be transplanted with epidermal cells + follicular buds,dermal cells alone,epidermal cells + follicular buds + dermal cells,follicular epithelial cells + dermal cells,respectively.The cells were implanted into the dorsal skin of nude mice using fold chambers.After the grafting,the growth of skin and hairs was observed at the grafted sites on week 1,2,4 and 8,and skin specimens were obtained on week 2,4,and 8 for histological study of hair follicles using hematoxylin-eosin staining.Results After grafting,the chambers on the back of nude mice began to shed with crust formation on week 1; stunted hairs came out and follicle-like structures were seen under the microscope on week 2 at the grafted sites,normal hairs were observed on week 4 and 8 in all the mice except for those transplanted with epidermal cells + follicular buds,and the growth of hairs in mice grafted with epidermal cells + follicular buds + dermal cells and mice grafted with follicular epithelial cells +dermal cells was superior to that in mice grafted with dermal cells alone.Conclusions Hair follicles can regenerate after hair follicle cell transplantation into dorsal chambers in nude mice.Both epidermal cells and dermal cells play indispensable roles in hair follicle reconstitution.