Objective To establish a cell line of human ovarian cancer, and study its characterization. Methods The cell line was established by the cultivation of subsides walls, and kept by freezing. The morphology was observed by microscope and electromicroscope. The authors studied its growth and propagation, the agglutination test of phytohemagglutinin (PHA), the chromosome analysis, heterotransplanting, immuno histochemistry staining, the analysis of hormone, the pollution examination and the test of sensitivity to virus etc. Results A new human ovarian carcinoma cell line, designated ovarian mucinous cystadenocarcinoma 685 (OMC685), was established from mucinous cystadenocarcinoma. This cell line had subcultured to 91 generations, and some had been frozen for 8 years and revived, still grew well. This cell line possessed the feature of glandular epithelium cancer cell. The cells grew exuberantly, and the agglutinating test of PHA was positive. Karyotype was subtriploid with distortion. Heterotransplantations,alcian blue periobic acid schiff (AbPAS),mucicarmine,alcian blue stainings, estradiol (E 2) and progesterone were all positive. Without being polluted, it was sensitive to poliovirus Ⅰ, adenovirus 7 and measles virus. Conclusions OMC685 is a distinct human ovarian tumous cell line.

Objective To observe the change of housefly (Musca domestica) breeding in the pig manure treated ecologically with its larvae. Methods The number of eggs and the hatching rate of larvae in the treated manure were compared with that in the untreated manure. Results The number of eggs laid in the treated manure accounted for only 17^7% of the total eggs, while those in the untreated manure accounted for 82^3%. The hatching rate in the treated manure was 41^4%, but 85^1% in the untreated manure. Conclusion There is a significant reduction of eggs laid and of their hatching rate in the pig manure treated ecologically by housefly larvae.

This paper explicated the present application of poly- vinyl alcohol (PVA) in the field of biomedical engineering, such as artificial cartilage, drug delivery systems, microorganism enwrapping, cell micro-capsulation, anti-thrombin materials, and biomedical sponges. And a preliminary study of the good dispersion of PVA as a surfactant on nano-particles of hydroxyapatite was presented.
Artificial Organs ; Biocompatible Materials ; Biomedical Engineering ; Drug Compounding ; Drug Delivery Systems ; Durapatite ; chemistry ; Materials Testing ; Nanoparticles ; chemistry ; Polyvinyl Alcohol ; chemistry

To explore the possibility of microencapsulation of chondrocytes in cartilage tissue engineering, immortalized manibular condylar chondrocytes (IMCCs) were microencapsuled by Alginate-polylysine-alginate (APA) method, according to air pressure shearing model. Phase contrast microscopy, trypan blue staining exclusion, cell number counting, HE staining and immunohistochemistry method were used to observe the morphology of the microencapsules, the growth character of cells, cartilage characteristics, and so on. The results showed that IMCC could survive and grow in microencapsule, and the viability rate of cells is more than 80 per cent. The diameter of microcapsule is 779 microns in average. The number of cell increased with time, and cells went into platform in about 20 days. Cells grew in clusters and cartilage specific proteoglycans and type II collagen were highly expressed. It was concluded that IMCC could form cartilage-like tissue within microencapsulation, implying that microencapsule technique might be applicable to cartilage tissue engineering.
Alginates ; Animals ; Cell Culture Techniques ; methods ; Cells, Cultured ; Chondrocytes ; cytology ; Drug Compounding ; Mandibular Condyle ; cytology ; Polylysine ; analogs & derivatives ; Rabbits ; Tissue Engineering ; methods

Objective:To investigate the clinical values of laboratory inflammation indicators including lymphocyte count, C reactive protein (CRP), serum amyloid A protein (SAA), and procalcitonin (PCT) in the diagnosis and treatment of 2019 novel corona virus disease (COVID-19) patients.Methods:The data of clinical characteristics of 77 hospitalized COVID-19 patients were collected from February to March 2020. According to the guidelines for diagnosis and management of COVID-19, these patients were divided into moderate type (49 cases) and severe type (28 cases). Among them 19 cases were discharged. The differences of lymphocyte count, CRP, SAA and PCT among moderate, severe and discharged groups were compared. The indicators with better predictive value for disease development and prognosis were screened out by logistic regression and receiver operating characteristic curve (ROC) analysis.Results:The severe patients had lower lymphocyte count compared to the moderate patients ( P=0.000), while their CRP ( P=0.000), SAA ( P=0.000) and PCT positive rate ( χ2=11.003, P=0.001) were significantly higher; The lymphocyte count of discharged patients was much higher than that of moderate ( P=0.001) and severe ( P=0.000) patients, while CRP ( P=0.036, P=0.000) and SAA ( P=0.002, P=0.000) were dramatically lower; Although the discharged patients had much lower PCT positive rate than severe patients ( χ2=6.891, P=0.009), they were not significantly different from moderate patients ( χ2=0.169, P=0.681). Logistic regression analysis showed that the increased SAA and CRP were both risk factors for predicting severe illness, but the decreased SAA were demonstrated as an independent indicator for discharge. The ROC curve analysis showed that the sensitivity and specificity of SAA for diagnosis of severe patients were 85.71% and 87.76%, whereas CRP were 82.14% and 93.88%. Further, the sensitivity and specificity of SAA for predicting discharge were 100% and 57.89%. Conclusions:The laboratory inflammation indicators such as lymphocyte count, CRP, SAA and PCT had important clinical significance for the diagnosis and treatment of COVID-19 patients. Among them, SAA was demonstrated as an independent indicator with high sensitivity for evaluating the disease progression and prognosis.