Background: Globally, researchers have found that while the use of synthetic drugs worldwide has increased by 2.1 2.5%, the use of natural medicines has grown by 3.5-4.8%. In modern times, the pharmaceutical industry has become more advanced, and the use of plant-based medicines is increasing. As a result, there is a growing need to identify biologically active compounds in medicinal plants and produce domestic pharmaceutical products to replace imported medicines. Due to the limited research on evaluating the quality of the raw materials of the Asian globeflower(Тrollius asiaticus L.) this study serves as a basis for assessing the quality of that flowers Aim: Determining the quality parameters of flower of Trollius asiaticus L. Materials and Methods: The research work was carried out with the support of the “Quality Control Laboratory for Medicine” of the School of Pharmacy, MNUMS. The quality assessment of flower of Trollius asiaticus L. was conducted according to established parameters outlined in the National Pharmacopoeia of Mongolia and Russian pharmacopeia and Chinese pharmacopeia methods. Parameters such as appearance, and microbiological purity were evaluated. Statistical analysis of research data was performed using Microsoft Excel and SPSS 25 software to calculate the mean, standard deviation, and percentage. Results: The flower of Trollius asiaticus L. is orange red or orange, plant does not contain organic and mineral impurities, the quality analysis of flavonoid compounds by the thin layer chromatography, a spot with RF=0.5125 was detected at the same level as orientin as a standard substance, and a spot with RF=0.875 was detected at the same level as gallic acid as a standard substance when analyzing the quality of polyphenolic compounds, moisture contain is 5.52%, sulfated ash contain is 9.12%, acid insoluble ash contain is 3.39%, water soluble extract contained 36.21%, the total polyphenolic compound contained 5.37±0.22%, total flavonoid compound contained 0.0399±0.013%. Conclusion The quality parameters of flower of Trollius asiaticus L. have been determined by the Mongolia National of Pharmacopoeia, the Pharmacopoeia of Russian and China.

Agleg-4 traditional medicinal drug consists from Smilax Glabra (Liliaceae), Rhododendron Adamsii (Ericaceae), Rheum undulatum (Polyganaceae), Glycyrrhiza uralensis (Fabaceae). This traditional medicinal drug’s humidity was 4.5%, ash was 5.4%, and extractive matter the in the water was 20.9%. The suitable thin layer chromatography system was selected as toluene: ethyl acetate: formic acid (7:2:0,5). From thin layer chromatography it showed flavanone glycosides. The highest content elements in the traditional medicine were Calcium 14mg/g, Potassium 3.2mg/g, Magnum 2.87mg/g. Agleg - 4 traditional medicine is increasing systolic action of heart so it is increasing kidney’s filtration and clearance and good medicine for kidney’s diseases.

Introduction Medicinal plants and herbal drug Introduction Medicinal plants and herbal drugs have made and a tremendous contribution to national health and development from the very beginning. Fora very long time, people have used locally available medicinal plants for medicaltreatment and have eaten certain vegetables and spices for health protection as well as sustenance. Even though in traditional medicine, we use many different medicinal plants and herbal preparations for treatment of various diseases, most of them are not completely studied yet. One of these herbal preparations is Jonsh-6. The research group reported already that the preparation Jonsh-6 has pharmacological effects such as to treat diseases such as gastritis, stomach disorder (B.Uyanga, S.Seesregdorj, 2011) and at this time there aren’t any other studies made in respect to the preparation Jonsh-6. The aim of our research is to complete qualitical and quantitative analyses of biological active substance in the preparation Jonsh-6. Material and methods The contents of the preparation Jonsh-6 are Calcitum-0.3g, Piper longim L- 0.08g, Aucklanda lappa Decne-0,15g, Inula helenium L-0.15g, Carthamus tinctorius L-0.24g, Amomum Kravanh Pierreex Gagnep-0.08g.Analyses of the preparation were studied by using HPLC on Waters Delta 600 Pump, Waters 600 Controller, Waters 2487 DualχAbsorbance Detector, Pinnacle II C18 5μm, 250x4.6 ммand selected following conditions: mobile phase: Acetonitrile:water/50:50,Acetonitrile:0.7% phosphoric acid /25:75,wavelength 210, 338, 403 nm, Detector: UVvis, column: Pinnacle IIC18 5μm (250x4.6 мм), flow rate 1.0 ml/min. Key words: HPLC, Jonsh-6, Safflomin-A, Piperine, Alantolaktone, and Costunolide. Conclusion In order to succeed the above mentioned aim, we have achieved the following results: 3. The chromatographical method and methodology forthe qualitical and quantitative analyses of main compounds in the preparation Jonsh-6 were developed using HPLC. 4. The quantity of main components in the preparation Jonsh-6 were determined with HPLC and Safflomin-A 1.06 mg, piperine 0.195 mg, alantolaktone 1.12 mg and costunolide 0.925 mg are contained in 1.003 gm of the preparation.

In last years we have produced a combination species from medicinal plants which are used in traditional medicine and has tendentious the increasing of needs and production of plant preparations from natural resources. Although traditional medicine has being increased to be used last years, standardization of them is not sufficient. The standardization is important to ensure the safety, effectiveness and quality of traditional medicine. This research was made with purposes for determining of qualitative and quantitative analyses of biological active substances in the preparation such as Jonsh-5 is utilized to prevent osteoporosis, to accelerate bone ankylose in period of injure. The compositions of the preparation Jonsh-5 are Calcitum, Carduus crispus L. Inula helenium L.Terminalia chebula Retz, Forsutima suspense Thunb. Vahl.In order to succeed the above mentioned aim, we have achieved the following results: 1. The chromatographical method and methodology for the qualitative and quantitative analyses of main compounds in the preparation Jonsh-5 were developed using HPLC. 2. Quantitative analyses of main compounds of the compositions in the preparations were defined by HPLC method. 1.000gr of the preparation Jonsh-5 involves gallic acid 1.11 mg, alantolaktone 1.77 mg.