Orientia tsutsugamushi is causative agent of tsutsugamushi disease, they parasitize in mites and is transmited from mother mite to other generation through egg (vertical transmission). In addition, they is transmited from this mite strain to other mite strain (horizontal transmission), this enssure large distribution of Orientia tsutsugamushi in mite community. However, this phenomenon rarely occure in nature. Orientia tsutsugamushi transmit to human through the bite of mites due to some of mite strains
Orientia tsutsugamushi ; Disease Vectors

The study was carried out in an endemic area of tsutsugamushi disease, Taketa city, Japan, from January to December, 2000. Results: 1018 unengorged larvaes of 14 species were isolated from soil and ground surface by direct method and Suzuki method. Among them Helenicula miyagawai was isolated by only the Suzuki method. Leptotrombidium scutellare and l.pallidum suspected as vectors of tsutsugamushi disease in many areas of Japan were detected in this study. The seasonal occurrence of unengorged larvae of L.scutellare was from October to January with the probable peak in November. While L.pallidum occur almost throughout the year with two peak periods, one in May and the other in September.
Scrub Typhus ; Trombiculidae

The study applied the Nested PCR technique with part of 56kilodalton type-specific antigen (TSA) gene DNAs of Orientia tsutsugamushi. Forty-eight pools (480 individuals) of unengorged larvae belonging to 4 trombiculid species: L. scutellare, L. pallidum, L. kistasatoi and L. fuji, collected from soil and ground surface of an endemic area for tsutsugamushi disease, were tested for 56kilodalton type-specific antigen (TSA) gene DNAs of Orientia tsutsugamushi, by nested PCR. Among them, 2 of 13 pools of L. scutellare were positive, and 2 serotypes of Kuroki and Gilliam were identified. Remaining 35 pools of 3 species were negative for Orientia tsutsugamushi. By using epidemiological' analysis, unengorged larvae of L. scutellare was suspected as the vector of tsutsu-gamushi disease in the studied area.
Orientia tsutsugamushi ; Scrub Typhus ; Epidemiology ; Polymerase Chain Reaction

Background: quality of food hygiene and safety directly affected public health. In Vietnam, after 5 years implementing the programs on ensure food safety, the aspects of food safety had improved significantly; food poisoning had been gradually controlled but still high. Objectives: to access on food poisoning outbreak in first six months of 2006 in Vietnam. Subjectives and Method: a cross sectional and retrospective study on food poisoning outbreak in first six months of 2006. The studied subjectives including: health staffs of provincial preventive centre, staffs in charge of food safety work; documents of food poisoning were stored in VFA. Results: there were 204 outbreaks in 58 provinces with 4.198 victims and 44 deaths or the mortality rate was 1.05%. By causes, percentages of outbreaks, cases and deaths due to bacterial pathogens were 41.7%, 53.4% and 0.0% respectively; due to chemical contamination: 12.7%, 13.0% and 4.5%; due to food denature: 9.3%, 17.2% and 11.4%; and due to natural toxicants: 34.8%, 15.2% and 81.4%, respectively. The number of deaths was highest in North-east and North-west areas (35 cases), in North and South Central coast (5 cases), and in Mekong river data (4 cases). Similarly, the mortality rates in these three regions were 5.0%, 0.49% and 0.27%, respectively. Conclusions: here were 204 outbreaks in 58 provinces with 4.198 victims and 44 deaths. Causes due to bacterial pathogens were the highest, followed by due to natural toxicants. The number of deaths was highest in North-highland.
Foodborne Diseases/epidemiology ;

Objective To study the clinical diagnosis value and CT features of gastric fundus and cardiac part lesions.Methods The CT findings and examining method of gastric fundus and cardiac part lesions were analyzed and summarized in 64 cases.Results (1)Use water as oral contrast can show the gastric mucosa clearly,it was a simple,effective way.(2)CT had important significance for stages and infiltration of cardiac cancer.(3)Varix of gastric fundus had three types:pseudotumor,tuber and thinking-wall.(4)The CT characteristic features of leiomyoma were the continuous linear enhancement in mucosa.Conclusion CT is method for gastrointestinal examining,it has special value to the gastric fundus and cardiac part lesions.

Objective To evaluate abdominal imaging in AIDS.Methods The imaging examinations(including US,CT and MR)of 6 patients with AIDS associated abdominal foci were analysed retrospectively.All the cases were performed US,and CT scan,of which 4 performed enhanced CT scan and 1 with MR.Results Abdominal tuberculosis were found in 4 patients,including abdominal lymph nodes tuberculosis(3 cases)and pancreatic tuberculosis(1 case).The imaging of lymph nodes tuberculosis typically showed enlarged peripheral rim enhancement with central low-attenuation on contrast-enhanced CT. Pancreatic tuberculosis demonstrated low-attenuation area in pancreatic head and slightly peripheral enhancement.Disseminated Kaposi's sarcoma was seen in 1 case:CT and MRI scan demonstrated tumour infiltrated along hepatic portal vein and bronchovascular bundles.Pelvic tumor was observed in 1 case:CT scan showed large mass with thick and irregular wall and central low attenuation liquefacient necrotic area in the pelvic cavity.Conclusion The imaging findings of AIDS with abdominal foci is extraordinarily helpful to the diagnosis of such disease.Tissue biopsy is needed to confirm the diagnosis.

OBJECTIVETo investigate the influence of isoliensinine (IL) on CYP3A enzyme activity.

METHODSA mixture metabolic system of liver microsome enzymes in vitro was developed. A HPLC method to test the metabolic activity of CYP3A was established with testosterone as a probe. The activities of CYP3A enzymes were measured with different IL concentrations and incubation time with testosterone.

RESULTSIn mixture metabolic system of liver microsome enzymes, the best incubation concentration of testosterone was 200 μmol/L, the best incubation time was 210 min, in this condition the IC₅₀ of IL for CYP3A inhibition was >1 000 μmol/L.

CONCLUSIONNo significant interaction between IL and CYP3A is detected, which indicates that IL might be used with CYP 3A enzyme substrates.

Animals ; Cytochrome P-450 CYP3A ; metabolism ; In Vitro Techniques ; Isoquinolines ; pharmacology ; Male ; Microsomes, Liver ; drug effects ; enzymology ; Rats ; Rats, Sprague-Dawley ; Testosterone ; pharmacology

OBJECTIVETo explore the inhibitory effects of Niaoluqing, an oral liquid of traditional Chinese medicine, on the growth of urogenital chlamydia trachomatis (Ct).

METHODSNiaoluqing's applying concentration was 1 g/ml and 10 serologically untyped strains of Ct from the STD clinic were used. And the inhibitory effects of Niaoluqing on Ct was evaluated by McCoy cell microculture technique in vitro.

RESULTSNiaoluqing had inhibitory activity for urogenital Ct, and was capable of reducing inclusion numbers notably in the concentrations of 50 to 200 mg/ml. The number and volume of Ct inclusions reduced gradually and disappeared finally with the rising of the medicinal concentration.

CONCLUSIONThe traditional Chinese medicine Niaoluqing has inhibitory effects on the growth of urogenital Ct.

Chlamydia trachomatis ; drug effects ; isolation & purification ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; pharmacology ; Female ; Humans ; Male ; Microbial Sensitivity Tests ; Urogenital System ; microbiology

The TCM theory "moxibustion shouldn't be applied at Shimen (CV 5) on female" is discussed in this article, which provides theoretical instruction on proper clinical application of Shimen (CV 5). Based on literature study, ancient classics concerning contraindication of "moxibustion shouldn't be applied at Shimen (CV 5) on female" were studied. TCM theory "moxibustion shouldn't be applied at Shimen (CV 5) on female" was explored profoundly through ancient record, physiological relations between Shimen (CV 5) and pregnancy, the relation between the anatomic structure of Shimen (CV 5) and the extra acupoint Jueyun Xue. And the result indicated that as an acupoint with contraindications, Shimen (CV 5) should be attached with great importance in its clinical practice on female patients. Reinforcing manipulation should be given instead of reducing. Moxibustion should be applied with cautiousness on the point, especially during pregnancy.
Acupuncture Points ; China ; Contraindications ; Female ; History, Ancient ; Humans ; Medicine in Literature ; Moxibustion ; history ; Pregnancy

Objective To retrospectively determine the features of stones and calcifications in hepatobiliary system on virtual nonenhanced (VNE) dual-energy computed tomography (CT), and to evaluate the possibility of VNE images in diagnosis for those lesions.Methods A total of 128 gall stones and calcifications of the liver found in 110 patients were examined with triple phase abdominal CT scan from July 2007 to December 2011, in which true nonenhanced (TNE) phase and arterial phase were performed with single-energy CT (120 kVp) and portal venous phase was performed with dual-energy CT (100 kVp and 140 kVp). VNE images were generated from the portal venous phase dual-energy CT data sets by using commercially VNC software. The mean CT values for the stone, liver, bile and paraspinal muscle, mean lesion density and size in area dimension, contrast-to-noise ratio (CNR) of lesion to the liver or bile, and image noise were assessed and compared between VNE and TNE images. The effective dose and size-specific dose estimate (SSDE) were also calculated.Results The mean CT values of the lesions measured on VNE images declined significantly compared with those measured on TNE images (164.51±102.13 vs. 290.72±197.80 HU, P<0.001), so did the lesion-to-liver CNR (10.80±11.82 vs.18.81±17.06, P<0.001) and the lesion-to-bile CNR (17.24±14.41 vs. 21.32±17.31, P<0.001). There was no significant difference in size of lesions area between VNE and TNE images (0.69±0.88 vs. 0.72±0.85 cm, P=0.062). Compared to the 128 lesions found in TNE images, VNE images showed the same density in 30 (23.4%) lesions, lighter density in 88 (68.8%) lesions, while failed to show 10 (7.8%) lesions, and showed the same size in 61 (47.7%) lesions and smaller size in 57 (44.5%) lesions. The CT cutoff values of lesion and size were 229.21 HU and 0.15 cm, respectively. The total effective dose for triple phase scan protocol with TNE images was 19.51±7.03 mSv, and the SSDE was 39.84±11.10 mGy. The effective dose for dual phase scan protocol with VNE images instead of TNE images was 13.29±4.89 mSv, and the SSDE was 27.83±9.99 mGy. Compared with TNE images, the effective dose and SSDE of VNE images were down by 32.05%±3.69 % and 30.63%±2.34 %, respectively.Conclusions Although the CT values and CNR of the lesions decreased in VNE images, the lesions of which attenuation greater than 229.21 HU and size larger than 0.15 cmcould be detected with good reliability and obvious dose reduction. There was good consistency in the size of stones and calcifications in hepatobiliary system between VNE images and TNE images, which ensured the possibility of the clinical application of VNE images.