6.Effect of Vagus Nerve Stimulation on Expression of 5-hydroxytryptamine 2A Receptor in Prefrontal Cortex of Coma Rats with Traumatic Brain Injury
Chinese Journal of Rehabilitation Theory and Practice 2016;22(4):404-408
Objective To explore the effect of vagus nerve stimualtion on wake-promoting and the expression of 5-hydroxytryptamine (5-HT) 2A receptor in the prefrontal contex of coma rats with traumatic brain injury. Methods 72 Sprague-Dawley rats were randomly divid-ed into control group, sham-stimulated group, stimulated group and antagonist group with 18 rats in each group. Traumatic brain injury mod-el was established by a weight-drop head injury. The antagonist group was injected with SB334867, and both the antagonist group and the stimulated group received vagus nerve stimulation. Their behaviors were recorded. And immunohistochemistry technique was used to detect the expression of 5-HT2A receptor in the prefrontal cortex. Results 12 rats in the stimulated group, 9 in the antagonist group and 4 in the sham-stimulated woke up. The expression of 5-HT2A receptor from low to high was ranged as the control group, the antagonist group, the sham-stimulated group and the stimulated group (χ2=11.464, P=0.009). Conclusion Vagus nerve stimulation could raise consciousness in co-ma rats after traumatic brain injury, which may be related to up-regulating the expression of 5-HT2A receptor.
7.Wake-promoting Targets for Deep Brain Stumulation (review)
Chinese Journal of Rehabilitation Theory and Practice 2016;22(7):801-803
Multi-tragets are used for deep brain stimulation (DBS) to promote wakefulness, such as midbrain, thalamus, hypothalamus, etc. Lateral hypothalamic area may be one of the most potential tragets.
8.Inhibiting the expression of proinflammatory factors in human nasal epithelial cells by dexamethasone
Chinese Archives of Otolaryngology-Head and Neck Surgery 2006;0(02):-
OBJECTIVE To observe the inhibition of the expression of HIF-1? and VEGF induced by LPS in primary human nasal epithelial cell (HNEC) by dexamethasone. METHODS Epithelial cells of nasal polyps (NP) and inferior turbinate (IT) were cultured without serum under stimulus of LPS 100 ng/ml, IL-1? 20 ng/ml, LPS 100 ng/ml +dexamethasone 13 ng/ml and IL-1? 20 ng/ml+ dexamethasone 13 ng/ml for 3h, 6h and 9h respectively. The expression of HIF-1?, VEGF protein and mRNA derived from epithelial cells was detected by immunocytochemistry and situ hybridization. RESULTS ①The expression of HIF-1? and VEGF increased under the stimulus of LPS and IL-1? with the increasing of time and concentration, especially under LPS 100 ng/ml for 6h (P