1.Study on the possibility of differentiation of bone marrow mesenchymal cells into endithelial cells in vitro
Orthopedic Journal of China 2006;0(01):-
[Objective]To study the possibility of differentiation of rabbit marrow mesenchymal stem cells(MSCs)into endothelial cells in vitro.[Method]BMSCs of rabbits were obtained by using gradient centrifuge method.The adhesive cells were preserved to passage culture to get pure MSCs.MSCs in the second generation were used.After induction with vascular endothelial growth factor(VEGF),microscopic exarm,cytoimmunofluorescent staining confirmd the trial group cells becomes to endothelial cells.W-P corpuscles which was the symbol of endothelial cells were observed by transmission electron microscope.[Result]The differentiated cells demonstrated the characters of endothelial cells under phase contrast microscopy.The cytoimmunofluore-scent staining showed that the trial group cells were induced to endothelial cells.W-P corpuscles could be seen under transmission electron microscope.[Conclusion]The adult rabbit BMSCs can present features of endothelial cells in certain induction medium,they are ideal donor cells of tissue engineering vascularization.
2.The Application of ER to Medical English Teaching
Chinese Journal of Medical Education Research 2002;0(01):-
This paper introduces the application of American TV series ER(Emergency Room)to the course of medical English. ER may not only arouse the students' interest in listening and speaking English but also help students learn medical terms,medical knowledge and medical ethics effectively.In addition,through ER students can learn how to deal with the relationship between doctors,nurses and patients.
3.Base and treatment of the thoracic myelopathy caused by ossification of ligamentum flavum
Orthopedic Journal of China 2006;0(05):-
The thoracic ossification of the ligamentum flavum(OLF)is usually located in the lower thoracic spine.Thoracic OLF has been widely recognized as a main cause of thoracic myelopathy.The pathogenesis of OLF is poorly understood.Because the thoracic OLF has many clinical manifestations and it is commonly combined with other spinal disorders,it is easy to be misdiagnosed or to be a delayed diagnosis.The surgical treatment of thoracic OLF has high risk and Serious complications.In this paper the pathogenesis,diagnosis and treatment of thoracic ossification of ligamentum flavum in recent years are reviewed.
6. Influence of self-designed three-dimensional woven scaffolds on in vitro growth of Schwann cells and its in vivo degradation
Academic Journal of Second Military Medical University 2010;30(10):1186-1190
Objective: To prepare a self-designed three-dimensional woven scaffold using poly lactide-co-glycolide acid (PLGA), and to observe the influence of the prepared scaffold on the growth of Schwann cells in vitro and its in vivo degradation. Methods: The 3D scaffolds were prepared by means of melt spinning, extension, weaving, and other procedures. The alignment of micro-tubules was observed under the scanning electronic microscope (SEM). The size of the micropores was also measured. Primary cultured Schwann cells were seeded on the 3D scaffolds, and the growth, adherence, proliferation, and apoptosis of Schwann cells were observed under inverted phase contrast microscopy and SEM; the results were compared between Schwann cells cultured in collagen sponge and culture dish. The scaffold carrying Schwann cells was implanted into the paraspinal muscle in rats, and H-E staining was used to observe the in vivo degradation and the inflammation responses. Results: The external diameters of the scaffold and micro-tubules were 3 mm and 100 μm, respectively, and the micro-tubules were arranged in an even and parallel manner. The adherence rates and proliferation rates of Schwann cells were similar between scaffold group and collagen sponge group, but both groups were significantly lower than the culture dish group (P<0.05). The 3D scaffold degraded completely within 12 weeks, with no visible inflammatory cells around. Conclusion: The self-designed 3D scaffold has no harmful effect on the growth of Schwann cells, and it can degrade in vivo, showing a satisfactory biocompatibility.
7.Clinical observation on heat-sensitive moxibustion plus lactulose for postoperative constipation of mixed hemorrhoid due to spleen deficiency
Journal of Acupuncture and Tuina Science 2020;18(3):219-224
Objective: To observe the clinical effect of heat-sensitive moxibustion plus lactulose oral liquid for postoperative constipation of mixed hemorrhoid.Methods: A total of 70 patients were randomized into a treatment group and a control group, with 35 cases in each group by a random number table. Patients in the control group received lactulose oral liquid alone, while those in the treatment group received additional heat-sensitive moxibustion at Shenque (CV 8). The treatment was given once a day for 15 consecutive days. The defecation interval time, defecation duration, the scores of stool form, visual analog scale (VAS) and patient assessment of constipation quality of life questionnaire (PAC-QOL), as well as the serum substance P (SP) and nitric oxide (NO) levels were measured before and after treatment. Results: After treatment, the stool form score, defecation interval time and defecation duration in the two groups dropped significantly (all P<0.05), and they were lower in the treatment group than in the control group (all P<0.05). After treatment, the VAS scores in the two groups dropped significantly (both P<0.05), and it was lower in the treatment group than in the control group (P<0.05). After treatment, the PAC-QOL scores in the two groups dropped significantly (both P<0.05), and it was lower in the treatment group than in the control group (P<0.05). After treatment, the serum SP and NO levels in the two groups improved significantly (all P<0.05), and showed statistically significant differences between the treatment group and the control group (both P<0.05). Conclusion: Heat-sensitive moxibustion plus lactulose oral liquid can improve stool form, shorten defecation interval time and defecation duration, alleviate defecation pain, and improve quality of life (QOL) for patients with postoperative constipation of mixed hemorrhoid, which may be related to the regulation of the SP and NO levels.
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