1."Research advances in ""treatment resistance"" of non-operative therapies for hepatocellular carcinoma"
Fudan University Journal of Medical Sciences 2017;44(3):374-379,394
Hepatocellular carcinoma (HCC) has the characteristics of high malignancy and poor prognosis.Liver transplantation and hepatectomy are applied in a small portion of patients with localized disease.Due to the hidden onset,HCC is usually diagnosed at an intermediate-advanced stage or with advancing cirrhosis.In most cases,various non-operative treatments are applied,including transarterial chemoembolization (TACE),local ablation,radiotherapy and molecular targeted drugs.The therapeutic effect of non-surgical treatments is not always ideal because the incidence of recurrence and metastasis after treatment is high.It also may be associated with treatment resistance of HCC to non-operative treatments.Optimizing the current management schemes has great significance in improving patients' quality of life and prolonging their survival.
2.Reflections on the development of trial-and-error video-type clinical skill training materials
Chinese Journal of Medical Education Research 2015;14(4):401-403
The training of clinical skill is closely linked with medical personnel education.However,traditional paper-type training materials were unable to meet the training needs.As a new type of training materials,trial-and-error video-type clinical skill training materials (abbreviated as TEVT clinical skill training materials) have gradually attracted attention with its characteristics of lively and interesting,potentially informative,high acceptance among students,the advantages' of stimulating students' initiative exploration and consideration,fostering students' critical spirit and humanities.On the basis of analyzing the advantages' of TEVT training materials,we discussed the developing specialties of TEVT clinical skill training materials from four aspects as the material orientation and the application object,material sources,constructing the developing team and editing the teaching materials.Furthermore,we reflected the application prospect of TEVT clinical skill training materials in the future,in order to explore an available implementation path for the development of TEVT clinical skill training materials.
3.Relationship between qi and blood quantity in the twelve meridians and musculature
Baoqiang DONG ; Ximing LI ; Gang DONG
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(02):-
Objective: To investigate the relationship between qi and blood quantity in the twelve meridians and musculature,and enrich the theory of meridian doctrine.Methods: By summarizing classic literatures on qi and blood quantity in the twelve meridians and musculature,and combining with clinical practice,the relationship between qi and blood quantity in the twelve meridians and musculature was analyzed and the directive effect of qi and blood quantity in the twelve meridians on diseases of musculature was investigated.Results: Qi and blood in the twelve meridians moisten and nourish the musculature,on the other hand,the musculature also influence the quality of qi and blood.Conclusion: Treatment for diseases of musculature with acupuncture and moxibustion can be perfected by the theory of qi and blood quantity in the twelve meridians,then,the curative effect can also be improved.
4.An analysis of test results of in-service training for stomatological professionals in Beijing community health service
Gang LIU ; Guangliang NIU ; Dong ZENG
Chinese Journal of General Practitioners 2009;8(3):182-183
In this paper we analyzed examination results of the Beijing Community Dentistry Professional Post Assessment and related factors, also discussed the measures for improving the quality of the post assessment. In 325 examinees 244 passed with a pass rate of 75.1%. There significant differences among examinees, educational background and academic training were most influential
5.Expression of nm23-H1 gene in oral squamous cell carcinoma and its relationship with metastasis to neck lymph nodes
Gang LIU ; Jinrong LI ; Yaojun DONG
Journal of Practical Stomatology 2001;17(1):24-26
Objective:To investigate the variation of nm23-H1 gene structure and its expression in oral squamous cell carcinoma (OSCC) and to study the relationship between nm23-H1 expression and OSCC metastasis to neck lymph nodes.Methods:nm23-H1 gene in the samples of 30 cases of OSCC,7 of normal oral mucous tissue,human OSCC cell line TSCCa and human oral metastatic cancer cell line GNM was extracted and amplified by PCR. PCR products were investegated with SSCP analysis;nm23 m RNA level in the samples was studied by in situ hybridization.The relationship between mRNA expression and metastasis to neck lymph nodes was analyzed statistically.Results: All samples showed no change in nm23-H1 gene structure except deletion in one case of OSCC;7 out of 9 cases with metastasis to neck lymph nodes showed no hybridization signal of nm23-H1 mRNA,however,only 5 were found no nm23-H1 mRNA in 21 cases without metastasis,positive rate of nm23-H1 expression in GNM cells was lower than that in TSCCa (41.2% versus 63.1%);The expression of nm23-H1 gene showed significant negative relationship with neck lymph nodes metastasis (P<0.01);no relationship was found between expression of nm23-H1 gene and cancer pathological classification.Conclusion:The change of nm23-H1 expression doesn't result from the variation of gene structure;the product of nm23-H1 gene probably inhibit OSCC metastasis to neck lymph nodes.
7.Cardiovascular Complications of Terminal Stage Cirrhosis and Related Clinical Significance
Chinese Journal of Bases and Clinics in General Surgery 2003;0(04):-
Objective To introduce the clinical significance and pathophysiologic aspects of the circulatory and cardiac complications in terminal stage cirrhosis.Methods Recently relevant literatures were reviewed and summarized.Results Haemodynamic changes in cirrhosis arose on the basis of combined humoral and nervous dysregulation,with abnormalities in cardiovascular regulation,volume distribution and cardiac performance.Conclusion Comprehending the mechanisms of cardiovascular complications will contribute a lot for the treatment of terminal stage cirrhosis.
8.Effects of MAPK on a human inflammatory breast carcinoma cell line
Huiming DONG ; Gang LIU ; Jiong WU
China Oncology 2001;0(05):-
Purpose:To invastigate the changes of the related signaling pathways and it's mechanism of reguation of mitogen activated protein kinase cascade(MAPK) by SUM149 cells induced plasmid of dominant-negative E-cadherin mutant H-2k~d-E-cadherin.Methods:Using western blot method,we examined the signal pathway of mitogen activated protein kinase(MAPK) between the dominant-negative mutant E-cadherin transfected SUM149 cells and controls.Results:Compared with the controls,the phosphorylated extracellular signal regulated kinase P44/42 expresion of the SUM149 cells which expressed higher dominant-negative mutant E-cadherin was significantly down-regulated.Conclusions:In this cell line model,the down-regulation of P44/42 may involve the regulation of MMPs.
9. Isolation and identification of human lung adenocarcinoma stem cells
Tumor 2008;28(1):1-7
Objective: To isolate and characterize the highly tumorigenic cancer stem cells with stem cell features from the established human lung adenocarcinoma cell lines. Methods: The cell subtypes were separated by magnetic activated cell sorting (MACS) technique. The stem cell-related markers on the isolated cells were detected by using flow cytometry and RT-PCR. The tumorigenic potent of the isolated cells was evaluated via the transplantation into NOD-SCID mice. Results: A novel cell subtype was identified in A 549 and SPC-A1 lung adenocarcinoma cell lines. These cells were characterized as a phenotype of CD 24+ IGF-1R+, possessed the property of high invasiveness and high tumorigenesis. Tumor could be induced in NOD-SCID mice by transplantation of CD 24+IGF-1R+ cells at 100 cells per mouse. The tumorigenicity of CD 24+IGF-1R+ cells had 1000-fold increase compared with CD 24-IGF-1R- cells. In addition, the CD 24+IGF-1R+ cells expressed embryonic stem cell markers (OCT 4 and Bmi-1) and lung stem cell markers (CCSP, SP-C, TTF-1, and Gremlin), suggesting that they had the features identical to the bronchioalveolar stem cells (BASC) in the lung of mice. These cells also exhibited autonomous growth features and could be cultured in serum-free conditions for a long time. Conclusion: The CD 24+IGF-1R+ cells in human lung adeocarcinoma belongs to the BASC-like cancer stem cells.
10.Clinical significance of preserving the intercostobrachial nerve in modified radical mastectomy for breast cancer
Gang DONG ; Wei CHAO ; Liming ZHANG
Journal of Clinical Surgery 2016;24(9):669-671
Objective To analyze and discuss the effects of preserving the intercostobrachial nerve(ICBN)in modified radical mastectomy for breast cancer. Methods A total of 80 patients with breast cancer were randomly divided into the observation group and control group(n = 40 in each). The ICBN in the observation group were reserved and it was removed in the control group. Operation time,the number of lymph nodes,blood loss and complication rates were compared between groups. Two groups of patients were followed up for abnormal sensory function and relapse and metastasis. Results There were no differences in the operation time,the number of dissected lymph nodes and blood loss(P > 0. 05). There was no difference in complication rate(12. 50% vs 15. 00% )between the observation group and control group(P > 0. 05). There were significant differences in the incidence of abnormal sensory function at the first week(10. 00% vs 52. 50% ),the first month(5. 00% vs 47. 50% ),the third month(2. 50%vs 45. 50% ),the sixth month(2. 50% vs 37. 50% ),and the twelfth month(0. 00% vs 27. 50% )after op-eration between the observation group and control group(P < 0. 05). During the one-year follow-up peri-od,there was no relapse or metastasis. Conclusion Preserving ICBN in modified radical mastectomy can effectively reduce the incidence of abnormal sensory function of the upper limb.