OBJECTIVETo investigate the osteoblast-like functional characteristics exhibited by human periodontal ligament cells (hPDLCs) under mechanical force.

METHODSHuman PDLCs cultured in vitro were stretched by mechanical force. Radioimmunoassay (RIA) was used to measure the expression of secreting alkaline phosphotase (ALP) and osteocalcin (OCN). The non-secreting ALP, OCN and osteopontin (OPN) in cells were determined by immunohistochemistry.

RESULTSIt exhibited increasing of ALP secreted into conditional media, and in the 24 hour period there were two peaks which appeared at the 2nd and 4th hour and the 24th hour (P < 0.01). While in the late of the 24 hours, expression of OCN in conditional media increased (P < 0.05).

CONCLUSIONMechanical force induces hPDLCs to differentiate into functional osteoblast-like cells and plays a role in bone remodeling.

Alkaline Phosphatase ; metabolism ; Cells, Cultured ; Humans ; Osteocalcin ; analysis ; Osteoclasts ; physiology ; Osteopontin ; Periodontal Ligament ; cytology ; Sialoglycoproteins ; analysis ; Stress, Mechanical

Objective:To understand the current situation and influencing factors of successful aging in widowed elderly.Methods:From May to October 2019, 225 widowed elderly people were investigated by questionnaire in 7 communities and 5 villages in Shandong Province. The Successful Aging Scale of the Flood was used to assess their successful aging status. The general data sheet, Physical Activity Scale for the Elderly (PASE), Multidimensional Scale of Perceived Social Support(MSPSS), Connor-Davidson Resilience Scale (CD-RISC) and Mini Mental State Examination(MMSE) were used to measure its influencing factors.Results:The successful aging score of the widowed elderly was 46.22 ± 9.49. The results of multiple linear regression analysis showed that the influencing factors of the successful aging of the widowed elderly were: social activity participation, household registration, consciously successful aging status, psychological resilience, MMSE—attention and computing power, perceived social support—other people, leisure physical activity, which can explain a total of 70.7% of the regression equation.Conclusions:The successful aging status of widowed old people is at a low level, and its influencing factors have both common factors and specific characteristics compared with those with spouses. In particular, more attention should be paid to "participation in social activities", and the widowed old people should be actively encouraged to participate and improve their successful aging.

This paper aimed at discussing the characteristics of blood glucose changes of type 2 diabetes according to the flow rule of Qi and Blood as well as the Hurst index of dynamic blood glucose collected by CGMS. A total of 156 T2DM patients were selected from the Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine. After continuous monitoring of dynamic blood glucose for 3 consecutive days (72 hours) with CGMS, the first complete 24 hour dynamic glucose, which start at 3 to 5 am(corresponding to Lung) and end at 1 to 3 am next morning(corresponding to Liver), are used for calculating each two-hours?? Hurst index. The measurement data was set at each two-hours, and multiple groups of related sample tests (Friedman) non- parametric test methods were used for comparison between groups. The statistical significance level was set to P ＜ 0.05. The results showed that the Hurst indices of each two-hour are all greater than 0.5. The three highest Hurst indices were: Stomach Hurst (0.960) ＞ Heart Hurst (0.946) ＞ Spleen Hurst (0.945), and the three lowest Hurst indices were: Lung Hurst (0.886) ＜ Liver Hurst (0.893) ＜ Large intestine Hurst (0.905). The standard deviation of the large intestine Hurst was the largest, reaching 0.088, and the standard deviation of the gastric Hurst was the smallest, 0.058. The distribution image of the Hurst index of individual patients at each twohours was similar to that obtained by the whole sample, but the specific time to a certain two-hours had fluctuations. The number of pairs with statistical difference between each two groups in two-hours is 29, accounting for 44％ of the total number of pairs. The organs and meridians that have more differences with other two-hours are successively stomach, lung, liver, kidney, spleen and heart. The differences of the blood glucose changes at different times in patients with type 2 diabetes can be indicated by the dynamic blood glucose Hurst indices, which are calculated according to the rule of Qi and blood flow.

Objective To study the effects of Guizhi Shaoyao Zhimu Decoction on factors related to bone destruction and bone protection in rats with collagen-induced arthritis(CIA)based on osteopro-tegerin(OPG)/receptor activator of NF-κB ligand(RANKL)/receptor activator of NF-κB(RANK)signaling pathway.Methods According to the body weight,60 female Wistar rats were randomly di-vided into the following six groups:the normal group,the model group,the Triperygium wilfordii mul-tiglucoside group(0.01 g/kg),the Guizhi Shaoyao Zhimu Decoction low-dose group(8.6 g/kg),the Guizhi Shaoyao Zhimu Decoction medium-dose group(17.2 g/kg),and the Guizhi Shaoyao Zhimu Decoction high-dose group(34.4 g/kg)(n=10 rats per group).The rats in all groups except for the normal group were given 100 μg bovine type Ⅱ collagen on the 1st and 8th days to establish the CIA model,and was injected into the left foot sole and tail root of the rats.After the successful modeling,the rats were treated by gavage for 4 weeks.The general state,body weight,and arthritis index(AI)score of rats were recorded,and the contents of RANKL and OPG in rat serum were determined by enzyme-linked immunosorbent assay.The mRNA and protein expressions of RANKL,RANK,and OPG in the ankle joint were determined through real-time PCR and Western blotting,respectively.Results Com-pared with the normal group,the general state of the model group was poor,the toe swelling was obvious,the AI score was increased,the serum RANKL content was increased,the serum OPG content was de-creased,and the mRNA and protein expressions of RANKL and RANK in the ankle joint were increased(P＜0.05).Compared with the model group,the degree of toe swelling and the AI score of rats in the Guizhi Shaoyao Zhimu Decoction low-,medium-,and high-dose groups were decreased,the serum RANKL content was decreased,the serum OPG content was increased,the mRNA and protein expres-sions of RANKL and RANK in the ankle joint were decreased,the mRNA and protein expressions of OPG were increased,and the RANKL/OPG ratio of the ankle joint was decreased(P＜0.05).Conclusion Guizhi Shaoyao Zhimu Decoction can improve the destruction of joint bone in CIA rats,and its mecha-nism of action may be related to reducing RANKL level,reducing RANKL/OPG ratio,and regulating bone balance.

Successful aging is an important strategy to actively deal with aging as well as a crucial measure to maintain national security and social harmony and stability, and the assessment of successful aging is the prerequisite and basis for relevant measures. However, standardized assessment tools for successful aging have not yet been formed by far, which has had a certain impact on the in-depth research in successful aging. This article summarizes and analyzes the comprehensive assessment methods and tools for successful aging at home and abroad, and provides references for the research into comprehensive assessment tools for successful aging, with a view to promoting the formation of standardized and comprehensive assessment tools for successful aging and developing comprehensive assessment tools for successful aging for Chinese elderly based on China's conditions.

[摘 要] 目的：探究益气活血汤对恶性肿瘤患者癌痛及癌因性疲乏（CRF）的疗效。方法: 选取2020年1月至2022年12月间安徽省中医药大学第二附属医院收治的82例确诊发生CRF的恶性肿瘤患者（气血亏虚证），采用随机数字表余数分组法将其分为对照组与观察组，每组各41例。对照组患者采用常规止痛、止吐、化痰等对症治疗及健康、心理指导，观察组患者在对照组干预的基础上联合益气活血汤治疗，4周为1个疗程。治疗前及治疗4周后，对两组患者进行中医证候积分评估，以积分变化评估中医临床疗效；采用修订版Piper疲乏量表（RPFS）评估CRF的改善情况；采用数字疼痛分级法（NRS）评分比较癌痛情况；检测患者外周血纤维蛋白原（FIB）、D-二聚体（D-D）评价凝血功能差异，检测患者肝、肾功能指标以评估益气活血汤治疗的安全性。结果：治疗前，两组患者在中医证候积分、RPFS评分、NRS评分及外周血FIB、D-D方面的差异均无显著统计学意义（均P>0.05）。治疗4周后，两组患者在神疲乏力、面色淡白或萎黄、自汗、失眠健忘、手足麻木的证候评分及总积分均较治疗前明显降低（均P<0.05），且观察组各项评分均低于对照组（均P<0.05），中医临床疗效明显高于对照组（P<0.05）；两组患者RPFS各维度评分及总分均较治疗前降低（均P<0.05），观察组行为、情感、感觉维度RPFS评分及总分均低于对照组（均P<0.05），观察组CRF的改善明显优于对照组（P<0.05）；两组患者NRS评分及外周血FIB、D-D指标均较治疗前降低（均P<0.05），且观察组均低于对照组（均P<0.05）。两组患者治疗期间均未发生肝功能、肾功能等明显异常，说明益气活血汤安全性良好。结论：益气活血汤可纠正气血亏虚之证，改善机体凝血功能，促进恶性肿瘤患者CRF及癌痛的减轻，临床应用价值较高。