Objective To optimize the extraction conditions for Yuemu eye drops.Methods The extraction conditions were studied with orthogonal test L_9(3~4),the content of caffeic acid was determined by HPLC as index.Results Alcohol concentration and frequency of extraction were remarkable factors in the test. Conclusion The optimum extraction technology for Yuemu eye drops was to reflux and extract the materials for 2 times in 80% alcohol,the volume of which was 6 times the weight of herbal medicine,with 1 hour for each time.

Iron overload is a metabolic disorder characterized by excessive iron deposition in the liver,pancreas,heart,endocrine organs, etc.,resulting in structural damage and dysfunction.The liver is the primary organ for iron storage,and excessive iron deposition induces liver inflammation and fibrosis,which may progress to cirrhosis and even liver cancer,with a poor prognosis.Accurate evaluation and effec-tive treatment can reduce liver injury caused by iron overload and improve patients′survival.

Through tracing to the source of syndrome differentiation according to meridian and collateral theory, Lingshu: Jingmai (Miraculous Pivot * Meridians) is studied in this article. Considered the requirement and concept of treatment according to syndrome differentiation, the application of proper syndrome differentiation methods based on meridian and collateral theory in acupuncture clinic is discussed. Theories, key factors as well as historical relations of syndrome differentiation according to meridian and collateral are also analyzed. The feature and origin of syndrome differentiation according to meridian and collateral, which are considered to be more important, are also discussed. Focuses of explanation are put on the functions, significances, connotations, relationship and clinical application of two major factors of syndrome differentiation, namely the running course of meridian and collateral and signs.
Acupuncture Therapy ; Diagnosis, Differential ; Humans ; Meridians ; Syndrome

To synthesize 3-nicotinamide-1, 2, 4-benzotriazine-1, 4-dioxide and to determine its anti-tumor activity in vitro. METHODS: Cyanogenamide and benzofuroxan were used as starting materials to prepare the intermediate (3-amino-1, 2, 4-benzotriazine-1, 4-dioxide, Tirapazamine). The objective substance was obtained from the reaction of the intermediate and nicotinic acyl chloride. The in vitro antitumor activities of the synthesized compounds were determined by MTT method using MCF-7 and HepG2 cells. RESULTS: The obtained compounds were identified by 1H-NMR, MS and IR. The purity of the objective substance determined by HPLC was 99.24%. CONCLUSION: The anti-tumor activity of 3-nicotinamide-1, 2, 4-benzotriazine-1, 4-dioxide is much better than tirapazamine. Copyright 2012 by the Chinese Pharmaceutical Association.

OBJECTIVE: To study the effects of total saponins of Panax japonicus (TSPJ) on human leukemic HL-60 cells. METHODS: Human leukemic HL-60 cells were cultured in vitro. The cancer cell vigor was detected by using cell counting kit-8. Nitroblue tetrazolium (NBT) was used for measuring cell reduction. The cell cycle and the expression of differentiation antigen CD11b were detected by flow cytometry. RESULTS: Compared with the negative control group, TSPJ in different concentrations could decrease the vigor of HL-60 cells and the number of cells in S phase and up-regulate the CD11b expression, while the numbers of NBT positive cells and cells in G(0)/G(1) phase in the different concentrations of TSPJ-treated groups were increased. CONCLUSION: TSPJ can inhibit the HL-60 cell growth in vitro. Its mechanism may be related to inhibiting proliferation and inducing cell differentiation and cycle arrest.

Objective To investigate the correlation of spiral CT(SCT)features with pathology, microvessel density(MVD)、expression of vascular endothelial growth factor(VEGF)、matrix metalloproteinase-2(MMP-2)in colorectal carcinoma.Methods Forty patients with colorectal carcinoma confirmed by operation were examined by SCT.The resected tumor specimens were immunohistochemically stained for the expression of VEGF,MMP-2 and the calculation of MVD.Results The accuracy of SCT in depicting the pericolic and wall infiltration was 92.5%.The metastasis rates of colorectal cancer with pericolic infiltration and wall infiltration were 75.0% and 33.3%,respectively,the differences were statistically significant between the two groups(P

Objective To study the validity of furosemide test for predicting the hematoma enlargement in pa- tients with hypertensive cerebral hemorrhage.Methods Four hundred fifty-one patients with hypertensive cerebral hemorrhage were diagnosed using CT after oneset of the disease and 24 h reexamined 24 h after.The incidence of enlarged hematoma was evaluated by comparison the baseline and 24 h CT scanning.Furosemide(20 mg iv)was ad- ministered and blood pressure was measured 30 min after furosemide.Results The decreasing level of MAP after furosemide was significantly inversely related with incidence rate of hematoma enlargement{ r=-0.94,t=58.4,P 10 mmHg as the cut-off point,with the rate of hematoma enlargement as being 6.4 %,MAP decreased≤10 mmHg was associated with increases in prevalence of hematome to 33.2 %(?~2=51.82, P

Due to unknown onset time, patients with wake-up stroke and unknown onset stroke areoften excluded from thrombolytic therapy. However, many clinical studies have shown that multimodal imaging techniques can effectively guide wake-up stroke and unknown onset stroke for intravenous thrombolysis. Its endovascular treatment has also made some progress. This article mainly reviews the imaging findings, intravenous thrombolysis, and endovascular therapy in patients with wake-up stroke and unknown onset stroke.

Objective To find out nutrient support drug utilization in cancer patients in our hospital. Methods An analysis was made by means of drug utilization research into the structure and rationality of nutrient support drug utilization in our hospital through an investigation into the detailed records of the dispensary from 1999 to 2002 and into 1 014 medical records from 2002. Results The variety, quantity, and cost of nutrient support drug utilization in the four years rose respectively by 58.82%, 71.66% and 28.16%. 49.30% of the cancer patients whose medical records were surveyed used such drugs. The quantity and person time ratio of PN and EN drug utilization was respectively 14∶1 and 13∶1, with malnourished patients accounting for 19.80%. Of the PN drugs whose DDD value could be determined, most had a DUI value that was greater than 1 whereas most of the EN drugs had a DUI value which was smaller than 1. Conclusion The variety, quantity, and cost of nutrient support drug utilization in the four years rose rapidly. The quantity and person time of PN drug utilization were much higher than those of EN drug utilization. Doubts could be cast in the handling of indications for nutrient support drug utilization in some patients and the doses of some nutrient support drugs prescribed were not so rational. Further explorations need to be made into the rational application of nutrient support treatment.

Objective To explore the causes and treatments for complications of laparoscopic incisional hernia repair.Methods A total of 110 patients with incisional hernia(42 men and 68 women,aged 33 to 89 years with a mean of 65)were treated with laparoscopic mesh repair in our hospital from March 2004 to May 2007.The clinical records of the patients were analyzed retrospectively.Results The laparoscopic repair was completed in all of the patients expect in one,who was converted to open surgery because of intraperitoneal adhesion.After the operation,15(13.6%)patients developed abdominal distension,86(78.2%)had severe pain in the operative area(not less than 6 weeks in 2 cases),23(20.9%)had seroma,6(5.4%)had urinary retention,2(1.8%)had acute gastric dilation,2(1.8%)had intestinal fistula,2(1.8%)had respiratory dysfunction,and 2(1.8%)showed recurrence of the hernia.Conclusion Most of the complications of laparoscopic incisional hernia repair are caused by insufficient preoperative preparation,intraperitoneal adhesion,difficulty in exposure of the visual field,improper use of electrocoagulation,small-sized masses,or weak fixation.