Objective To analyze the relative factors of bone marrow suppression after chemotherapy with different-dose carboplatin and paclitaxel (TC) on the patients with ovarian cancer.Methods Sixty-two patients with ovarian cancer admitted in Beijing Obstetrics and Gynecology Hospital from January 2002 to December 2007, using TC regimen ,a total of 196 cycles of chemotherapy ,were divided into two groups by the doses of carboplatin [area under concentration-time curve(AUC) 4 -6 for low-dose,AUC >6 -7 for hight-dose, the carboplatin dose calculated with AUC] or by the doses of paclitaxel (135 -< 150 mg/m2 low-dose,150 - 175 mg/m2 hight-dose). After each TC cycle, the routine blood was test to determine the graduation of the marrow suppression, and then the correlation factors were analyzed with logistic regression. Results (1) The occurrence rate of bone marrow suppression:there were 159 cycles (81.1%) grade 0 - Ⅱ bone marrow suppression, while 37 cycles (18.9%) of grade Ⅲ - Ⅳ. (2) Factors related to bone marrow suppression:the results shown that there were not related to bone marrow suppression,which incluced cellular differentiation, tumor type, height, weight and paclitaxel dose(P>0.05). While,the different cycle, age, the later stages of tumor, serum creatinine concentration, endogenous creatinine clearance rate, AUC values were the relative factors of bone marrow suppression(P =0.000,0.000,0.018,0.033,0.001,0.000). Seven variables were conducted into the logistic regression and the results shown that the different cycles, the age, AUC grades were independent risk factors (P = 0.030,0.043,0.009).(3) When low-dose of paclitaxel was given, the occurrence of bone marrow suppression was related to the carboplatin dose AUC. The higher AUC values for carbopaltin were chosen, the higher of severe bone marrow suppression would happen. (4/14 vs 0,P=0.015). When the dose of high grade of paclitaxel was given, the occurrence of bone marrow suppression in cases with hight-dose carboplatin was statistically significant than that in cases treated with low-dose carboplatin [45.7%(16/35) vs 13.7% (17/124), P=0.000]. Conclusions The independent risk factors of myelosuppression after chemotherapy with TC regime on the patients with ovarian cancer including the cycles, age and AUC values. The carboplatin dose calculating with AUC is related to the occurrence of bone marrow suppression, the higher AUC values for carbopaltin would chosen,the higher of severe bone marrow suppression would be happen.

ObjectiveTo explore the ability of inhibition resistibility of multiple displacement amplification (MDA)in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods(MBM)to purify sample.MethodsDifferent concentra-tions of hemoglobin and humid acid(HA)mixed with DNA samples and then divided the samples into MDA group, MBM group and control group.D3S1358locus was amplified and detected by polyacry-lamide gel electrophoresis detection system and AmpF?STR? IdentifilerTM Plus Kit-capillary electrophore-sis detection system.ResultsWhen hemoglobin concentrations exceed 1 ng/μL or HA concentrations ex-ceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.ConclusionMDA has the capability to remove the inhibi-tion of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.

Objective To observe the efficacy and side effect of olanzapine combined with tropisetron, dexamethasone for the pre-vention of highly emetogenic chemotherapy-induced nausea and vomiting ( CINV) . Methods A total of 78 patients with highly emetogen-ic single day chemotherapy were randomly divided into two groups:trial group ( olanzapine combined with tropisetron, dexamethasone n=40) and control group (Tropisetron Combined with Dexamethasone n=38). The control of acute CINV, delayed CINV and adverse reac-tions were observed. Results The control rates of acute vomiting in trial group and control group were 87. 5% vs 81. 6%, 65. 0% vs 57. 9% in acute nausea, 75. 0% vs 52. 6% in delayed vomiting, 32. 5% vs 13. 2% in delayed nausea. The trial group was better than the control group in delayed vomiting and delayed nausea which there was difference between them (P < 0. 05). There was no statistical differ-ence between the two groups in the incidence of adverse reactions (P>0. 05). Conclusion Olanzapine combined with tropisetron, dexa-methasone for the prevention was significantly better than tropisetron combined with dexamethasone in the control of delayed CINV in pa-tients received highly emetogenic chemotherapy.

The morbility of bronchial asthma is gradually increasing year by year all over the world. Numerous studies have demonstrated that bronchial asthma is close related with immunoregulation. Vitamin D3 is involved to immune regulatory mechanisms in bronchial asthma, including regulating cytokine, Treg, matrix metalloproteinases-9. Considering the influence of vitamin D3 on the immune regulatory of bronchial asthma, it is provided a theory evidence that vitamin D can be used in the treatment of bronchial asthma effectivly and a novel method of immune treatment for children with bronchial asthma eventually.

Child ; Child, Preschool ; China ; epidemiology ; Evidence-Based Medicine ; Humans ; Kidney Diseases ; diagnosis ; epidemiology ; therapy ; Practice Guidelines as Topic

Objective To explore the effect of yam polysaccharide for the diabetic mice. Methods Ninty mice were randomly divided into three groups ,yam polysaccharide group(n = 30), glibenclamide group (n = 30) and control group(n = 30). Mice were Intraperitoneal injection of alloxan (200mg/kg) to establish diabetic model. Yam polysaccharide group was administered yam polysaccharide for 12d. Results Yam polysaccharide significantly decreased the blood sugar of the diabetic mice. There were not significantly different in two groups (P ＞ 0.05).Conclusion Yam polysaccharide had obvious role in decreasing blood glucose.

Lung cancer-related papers published by authors of China were retrieved from PubMed and the high frequency subject headings and subheadings were extracted from them using the Bicomb system.The status quo and hot spots of lung cancer-related studies in China were analyzed .

The United States is the first country that has introduced legislation designed to stimulate orphan drug research and development .In this paper , we analyze three major amendments of the Orphan Drug Act ( ODA) since 1983 and its incentive system .We then discuss the success of the ODA in encouraging orphan drug research and development and boosting the biopharmaceutical industry .Following that , we investigate the high price of orphan drugs caused by market exclusivity and drug repositioning .Finally, we draw experience from the ODA to make some suggestions on how orphan drug legislation can be devised in China with the aim of improving the health of rare -dis-ease patients and facilitating the development of Chinese biopharmaceutical industry .

ObjectiveTo evaluate the role of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) for diagnosing lesions of gastrointestinal tract and the nearby organs. MethodsClinical and pathological data of 161 EUS-FNA lesions in 133 consecutive patients were retrospectively analyzed. ResultsOf the 161 lesions, there were 142 solid lesions, 15 cystic lesions and 4 pancreatic solid-cystic lesions. EUS-FNA was performed at lesions of upper gastrointestinal tract, rectum and anal canal. 160 cytologic diagnoses (99. 4％ ) were obtained in all 161 lesions. 143 histological diagnoses (97.9％) and 113 immunohistological diagnoses (77.4％) were obtained in 146 solid lesions (including 4 pancreatic solid-cystic ones). Diagnosis rates of samples for immunohistology remained similar between 22-gauge and 19-gauge needles and also similar among different sites (P ＞ 0. 05). But immunohistological diagnosis rate of smaller lesions ( ＜ 1 cm) was lower than that of bigger ones ( ≥ 1 cm) ( P ＜ 0. 05). The diagnosis rates of cytology,histology and pathology in solid lesions were 81.2％ ( 120/146), 82. 9％ (121/146) and 89. 7％ ( 131/146), respectively. Size of needles and lesion location did not exert influence on diagnosis, but the diagnosis rate of smaller lesions ( ＜ 1 cm) was lower than that of bigger ones ( ≥ 1 cm). Cytological diagnosis was consistent with histological diagnosis ( k =1. 0, P =0. 00), 11 cases that could not be diagnosed by cytology was confirmed by histology and 10 cases that could not diagnosed by histology was confirmed by cytology.There were no complications during the procedures. ConclusionEUS-FNA is a valuable diagnostic tool for lesions of gastrointestinal tract and nearby organs. The needle size and lesion site were not influential factors for diagnosis. Smaller lesions ( ＜ 1 cm) yielded lower diagnosis rate than bigger ones ( ≥ 1 cm). The cytologic diagnosis combined with histology can improve the diagnostic value of EUS-FNA.

Objective To discuss the effect of project management on improvement of the success rate of one trial urine catheterization and reduction of complications caused by catheterization care for male patients with liver failure.Methods Applying project management to train 14 registered nurses about the standard method of male patients' catheterization,then the mastering of the 6 points of catheterization were evaluated.From May 2009 to May 2012,146 male patients in our department were divided into the control group (84 cases)and the observation group (62 cases).The success rate of one trial urine catheterization and major nursing complications were evaluated.Results After using the project management,nurses could make significant improvement in mastering the 4 points,as the technique of lubricating the urethra,giving the assessment to urethral mucosa of the patients with liver function as Child-pugh C grade,common complications which caused by operation of catheterization and its circumvention technology.The success rate of one trial urine catheterization was higher,the injury rate of urethral mucosa and pain degree were lower in the observation group than those in the control group.Conclusions Project management can significantly improve the clinical operation capability of nurses and effectively decrease the catheterization complications.