To observe the application effect of anti-eyebrow small incision cataract extraction combined with lOL implantation in blindness prevention and treatment
●METHODS: A total of 526 cataract patients included in regaining sight project of China Disabled Persons' Federation were underwent anti - eyebrow small incision cataract extraction combined with lOL implantation from May 2010 to August 2013.
●RESULTS: Postoperative 1d, uncorrected visual acuity >0. 3 were 345 cases (65. 6%), 0. 1 - 0. 3 were 152 cases (28. 9%), <0. 1 were 29 cases (5. 5%); 1wk after surgery:>0. 5 were 395 cases (75. 1%), 0. 3 - 0. 4 were 101 cases (19. 2%), < 0. 3 were 30 cases (5. 7%). Visual recovery rate was 100%, residual rate was 92. 5%. lntraoperative, 16 cases occurred posterior capsular rupture, 3 cases iridodialysis, 2 cases were Descemet's membrane avulsion. Postoperative, 56 cases ocurred corneal edema, 10 cases of central cornea stroma edema, 22 cases of secondary high intraocular pressure, were restored to normal bansis on the corresponding treatments.
●CONCLUSlON: Anti - eyebrow small incision cataract extractio combined with lOL implantation is effective, and has low complication rate, high security, can popularization and application in large - scale sight rehabilitating project.

The purpose of the present study was to assess the correlation that likely exists among increased portal pressure (Pp), portal blood flow quantity (Qp) and ETA and ETB receptor mRNA expression in human cirrhosis. In situ hybridization and reverse-transcription polymerase chain reactions (RT-PCR) were performed to determined the expression of ETA and ETB receptor mRNA in liver tissues from traumatic subjects (n = 10) and cirrhotic patients (n = 15) in whom hepatic hemodynamic values were measured. The expression of the two transcripts was significantly higher in liver samples of cirrhotic patients than in those obtained from traumatic subjects. It has shown that ETA receptor mRNA predominantly located in hepatic stellate cells (HSCs) and vascular smooth muscle cells of intrahepatic arteries and portal veins, ETB receptor mRNA in HSCs, sinusoidal endothelial cells and Kuppfer cells. There was a highly significant direct relationship between ETA and ETB receptor mRNA and Pp and Qp in cirrhotic patients. It suggests that liver paracrine endothelin system may be overactivated in human cirrhosis accompanied with increased expression of ETA and ETB receptor mRNA which may play an important role in the pathogenesis and maintenance of splanchnic hyperdynamics.
Gene Expression ; Hemodynamic Processes ; Hypertension, Portal/metabolism ; Liver Cirrhosis/genetics ; Liver Cirrhosis/*metabolism ; Portal Vein/*physiopathology ; Receptors, Endothelin/genetics ; Receptors, Endothelin/*metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; *Splanchnic Circulation/physiology

OBJECTIVE:To prepare and characterize lansoprazole(LAP) cationic liposomes. METHODS:Liposomes were prepared by ethanol injection technique.An orthogonal test was utilized to optimize the formulation and preparation of LAP liposomes.The encapsulation efficiency was determined by ultrafiltration.The morphological examination of LAP liposomes was performed using transmission electron microscopy.The particle size and Zeta potential of the liposomes were measured.The release rate of LAP from liposomes was tested. RESULTS:The liposomes with spherical or ellipsoidal shape and better stability featured the encapsulation efficiency of(80?1.23)%,the mean partical size of (184?21)nm,and Zeta potential of (36.1?5)mV.The release kinetics in vitro obeyed first-order equation.The stability of LAP was better. CONCULSION:The selected formulation and preparation technic of lansoprazole liposomes were rational and stable and liposomes featured a sustained release in vitro.

0.05),and during which,no adverse drug reactions were observed.The prophylactic medication days,antibiotic drugs costs and total drugs costs in hospitalization were significantly lower in the trial group than in the control group(P

Objedive To observe the effect of microRNA-29c cm the proliferalion and invasion abililies of hirman prostate carreer cell litre LNCaP, and to discijss its potetrlial applicasion. Methods The difference in miR-29c expression between ADPC (androgen-dependent prostate cancer) and AIPC (androgen-independent prostate cancer)cell lines was observed by real time RT-PCR.MiR-29c-inhibitor (anti-miR-29c) was used to decrease miR-29 cexpression in LNCaP cells; then the cell proliferation was examined with CCK-8 assay, the cell cycle was analyzed using flow cytometry, and the invasive abilities of LNCaP cells were observed by transwell invasion assay before and after treatment with anti-miR-29c.Results The result of real-timeRT- PCR showed that miR-29c expression in LNCaP-AI cells was significantly lower than that in LNCaP cells (P<0.05).Down- expression of miR-29c in LNCaP cells significantly promoted the proliferation and invasion abilities of LNCaP cells (P<0.05). Conclusion Normal expression of miR-29c plays an inhibitory role in the proliferation and invasion of LNCaP cells, in dicating it might be a new target for biotherapy of prostate cancer.

Objective To observe the effects of basic fibroblast growth factor (bFGF) on osteogenic differentiation abili?ty and cell proliferation of human gingival fibroblasts (HGFs), and to explore the role of bFGF on the process of osteogenic differencitiaion in vitro. Methods HGFs were cultured in vitro until the 3rd passage when they were divided into four groups:normal medium as group 1, normal medium with 10μg/L bFGF as group 2, osteogenic medium as group 3 and osteo?genic medium with 10μg/L bFGF as group 4. MTT assay was used to evaluate the proliferation of HGFs. Alkaline phospha?tase (ALP) staining and Alizarin red staining were applied to investigate osteogenic potential of HGFs under different culture conditions. Results bFGF at concentration of 10 μg/L could increase HGFs proliferation in both normal and osteogenic medium (P<0.01). HGFs could be induced towards osteogenic differentiation and form mineralized nodule in osteogenic me?dium. However, 10μg/L bFGF had no effects on ALP activity and mineralized nodule formation of HGFs during osteogenic differentiation. Conclusion bFGF could promote the proliferation of HGFs but show no effects on osteogenic differentiation of HGFs at concentration of 10μg/L.

Objective Increased morbidity of auto-immune disease in senescent individual might be related with the defects of apoptosis after stimulation or induction. Changes of apoptotic features in splenic T lymphocytes were studied in senescent mice. Methods Flow cytometry was used to study the rates of apoptosis by analysing sub-G 1 peak. DNA ladder was observed by agarose gel electrophoresis. Free calcium levels in both cells were detected by Fluo-3 loading. Bcl-2 protein levels were detected by Western blot. Results After co-stimulation with IL-2/ConA, flow cytometry showed that average apoptotic percentage of old T cells was 38.3% ?10.3%,significantly lower than that of young ones (58.6% ? 4.0%, P

BACKGROUND: Osteogenic growth polypeptide (OGP) had clear effect on promoting osteoblast proliferation, differentiation and mature. OBJECTIVE: To explore the expression of OGP gene, which was transfected into rabbit bone marrow mesenchymal stem cells (BMSCs) and to evaluate the effects of OGP on differentiation of rabbit BMSCs. METHODS: pcDNA3.1-OGP was constructed using gene cloning and recombination techniques. Rabbit BMSCs were transfected with pcDNA3.1-OGP mediated by lipofectamine 2000. The transfection positive cell clones were selected with G418. The expression of OGP gene was detected using reverse transcription-polymerase chain reaction analysis on an mRNA level. Differentiation of pcDNA3.1-OGP transfected BMSCs into osteoblast lineage was observed. RESULTS AND CONCLUSION: The pcDNA3.1-OGP plasmid was constructed successful and OGP expression was detected in rabbit BMSCs. Hydroxyproline content was increased, and alkaline phosphatase activity was also increased. These indicate that pcDNA3.1-OGP transfected BMSCs expressed OGP, and could differentiate into osteoblast lineage.

[Objective] To study the effects of Buyang Huanwu Decoction and Radix Astragali on astrocytes in gerbils with cerebral ischemia and reperfusion injury. [Methods] Gerbils model of cerebral ischemia was set up by occlusion of bilateral common carotid arteries. The dynamic expression of glial fibrillary acidic protein (GFAP) were determined by immunohistochemical method in reperfusion for 24 and 48 hours after 15 minutes of cerebral ischemia. [Results] Positive expression of GFAP reached a peak in reperfusion for 24 hours and was decreased by Buyang Huanwu Decoction and Radix Astragali. Positive GFAP expression was attenuated in reperfusion for 48 hours and enhanced by Buyang Huanwu Decoction and Radix Astragali increased the expression. [ Conclusion ] The regulatory effect of Buyang Huanwu Decoction and Radix Astragali on astrocytes may be one of its mechanisms in repairing nervous function after cerebral ischemia.

Abstract: Objective　To investigate the impacts of vaccination with inactivated SARS-COV-2 vaccine on the clinical manifestations and serological responses of COVID-19 patients infected by Delta and Alpha variants. Methods　Clinical and experimental data of 341 confirmed SARS-COV-2 patients were collected from The Eighth Affiliated Hospital of Guangzhou Medical University May 1- September 30, 2021. The subjects were divided into Delta and Alpha variant group according to virus variants, and were divided into vaccinated group and unvaccinated group according to whether they had received inactivated COVID-19 vaccine or not. The clinical manifestations and serological responses of patients with Delta and Alpha variant, and vaccinated and unvaccinated patients with Delta and Alpha variants were compared. Results　Totally 253 patients were infected with Delta variant (103 vaccinated and 150 unvaccinated patients), and 88 patients were infected with Alpha variant (21 vaccinated and 67 unvaccinated patients). The proportion of asymptomatic infection in Delta variants group was significantly lower than that in Alpha variants group (P<0.01). Delta variant group of vaccination rates and vaccine breakthrough infection rate was 40.7% (103/253) and 22.9% (58/253), were higher than Alpha variant group was 23.9% (21/88) and 8.0% (7/88), difference was statistically significant (χ2= 8.009, 9.484, P<0.01). The proportion of cough and fever in Delta variant group was higher than that in Alpha variant group (both P<0.01), the peak viral load was higher than that of Alpha variant group (P<0.01), the virus duration was longer than that of Alpha variant group (P<0.01), the levels of SAA, CRP and IFN were higher than those of Alpha variant group (all P<0.05), CD4+T cell count was lower than that of Alpha variant group (P<0.05), IgG and IgM levels were lower than those of Alpha variant group (both P<0.01). The proportion of moderate COVID-19 in the vaccinated group was lower than that in the unvaccinated group (P<0.01). In these two variants, the peak viral load of vaccinated group was lower than that of the unvaccinated group (both P<0.01), the duration of virus was shorter than that of unvaccinated group (both P<0.01). The levels of SAA, CRP and IL-6 in the vaccinated group were lower than those in the unvaccinated group (all P<0.05), CD4+T cell level was higher than that of unvaccinated group (both P<0.05), IgG and IgM level were higher than those in unvaccinated group (both P<0.05). Conclusions　Delta variant can lead to higher viral load and more severe disease course, which is associated with vaccine breakthrough infection. Inactivated vaccines for COVID-19 can reduce severe illness and death by reducing viral load, disease duration and inflammatory response through humoral and cellular immune mechanisms.