Vascular Endothelial Growth Factor and its receptors may be the pivotal stimulants in the process of growth and metastasis of a neoplasm. They boost the genesis of the blood vessels of brain glioma. Their expression is correlated with the grading of glioma. They are ideal targets in the treatment of inhibiting the growth of the blood vessels of brain glioma.

As an important acupoints beyond meridians, Huatuo spine points are used extensively. Its localization generally is considered on the back waist where is under the both sides of the spinous processes (0.5 inch) from the first thoracic vertebral to the fifth lumbar. Furthermore, Huatuo spine points include 17 points on the right side and 17 points on the left. Through a large number of clinical observations and literature’s searching, the writer considers that the Huatuo spine points are an integrated, independent region that is combined pass through the Bladder meridian and the Du meridian. Therefore, acupuncture the region may depend on the specific conditions.

Objective To study the effect ofQianzheng powder through point directional transdermal drug delivery technique for the patients with urge incontinence(UI) after stroke.Methods Seventy patients with UI after stroke were randomly divided into treatment group and control group(each group 35 patients). Qianzheng powder through point directional transdermal drug delivery was given to the treatment group, but the tolterodine through mouth was given to the control group. The urinary diary and bladder function at baseline and after 2 weeks' treatment were observed.Results After treatment, the total effect rate(88.2%vs. 63.6%,χ2=5.575) in the treatment group were better than that in the control group (P<0.01).The duration of voided (17.05 ± 6.21 times/48 hvs. 28.63 ± 9.62 times/48 h,t=5.872), the times of urinary incontinence (2.08 ± 1.24 times/48 hvs. 7.19 ± 2.46 times/48 h,t=10.784), the average voided volume (282.38 ± 14.32 mlvs. 185.82 ± 15.43 ml,t=26.561), the times of nocturia (1.38 ± 0.34 times per nightvs. 2.45 ± 1.56 times per night,t=3.906), the initial Sensory capacity of bladder (246.76 ± 21.95 mlvs. 176.43 ± 20.64 ml,t=13.502), the maximum capacity of bladder (495.80 ± 26.72 mlvs. 295.81 ± 28.46 ml,t=29.663), the residual urine volume (12.25 ± 6.83 ml vs. 33.63 ± 7.25 ml,t=12.428) in the treatment group showed significantly more improvement than those in the  control group (P<0.01).Conclusions TheQianzheng powder by point directional transdermal drug delivery could reduce the symptoms of urinary incontinence, and improve the life quality of patients with UI after stroke.

Acupuncture has a definite curative in treating pain.Pain is a subjective feeling,which is not easy to be measured because of lack of quantifiable index.Besides,because evaluation measures for acupuncture analgesia are not unanimous,it is lack of comparability among the different clinical researches.The author summarized the clinical evaluation measures of acupuncture analgesia which are used in clinical commonly,and considered that the clinical study on acupuncture analgesia should be globally evaluated from the extent of pain,complication symptoms and life quality on the basis of determining the nature and xing quantity of the subjective index.

The acupuncture manipulation is an important factor for achieving a good therapeutic effect in acupuncture practice.The authors review recent studies on acupuncture manipulation and its qualification from a) differences of the therapeutic effects of acupuncture manipulation,b) quantification of acupuncture manipulations,and c) instruments for delivering and sampling the analogued parameters of different acupuncture manipulations,computer analysis software,etc.Most studies revealed that different acupuncture manipulations had different clinical efficacies.Meanwhile,researches on the quantification of acupuncture manipulations and some related instruments also have made a bigger progress.Nevertheless,there still exist many problems,such as difficulties of various acupuncture techniques in unification in clinic,stronger personal subjectivity in performing manipulations,etc.We should overcome these problems and conduct more researches,so as to raise the clinical curative effect further.

Objective: To explore the changes of activity of antioxidases in hippocampus of the rats with multi-infarct dementia and the intervention effects of acupuncture.Method: The multi-infarct dementia model was made by injected emboli into the internal carotid artery.The demented rats were allocated to 3 groups randomly: model,acup and sham-acup;meanwhile normal and sham-operated rats were used as control.The parameters of oxidative stress were assessed by measuring the malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GSH-Px) levels in hippocampus after acupuncture treatment.Result: Compared to normal group,MDA levels of hippocampus in model animals increased significantly,while SOD and GSH-Px decreased much more;No significant difference was observed in CAT among groups.Interestingly,the acupuncture treatment could significantly improve all those indexes.Conclusion: SOD/GSH-Px pathway may be playing a major role in combating oxidative stress in the brain of MID rats,the CAT activity not the key enzyme.Acupuncture treatment could increase the activity of antioxidase SOD/GSH-Px,alleviate the injury of free radical and maintain the oxidant-antioxidant balance to a great extent.This may be one of the mechanisms of its therapeutic effect.

Migraine is the most common type of primary headache.The ideal animal models of migraine should be able to well replicate the key pathophysiological mechanism of migraine.According to the different pathogeneses,this article reviews the advances in research on the animal models of migraine from the aspect of making methods,determination criterion,advantages and disadvantages,and the scope of application,

Objective To evaluate clinical significance of the CEA, CA19-9, CA242, AFP, CA724,SCC, TPA, TPS combined detection for the clinical diagnosis of gastrointestial cancer. Methods 373 cases of upper digestive tract malignant tumor patient and 50 cases of healthy serum sample were detected for eight markers, evaluating the relationship between the level of markers and illness. Results 8 marker diagnostic positive rates were: CEA 26.80 %, CA19-9 27.34 %, CA242 34.14 %, AFP 2.84 %, SCC 19.72 %, CA724 12.13 %, TPA 34.15 %, TPS 30.89 %. The total positive rate of joint detection of 8 markers was 89.05 %.Preoperative CEA, CA242, SCC-positive patients had a shorter survival time. CA242 and CA19-9 levels with upper gastrointestinal malignancies were positively correlated. Conclusion The combined detection of a number of markers in different pathological type and clinical staging is statistically significant, and the positive rate is much higher than any single marker test results. CA19-9 and CA242 may be the best combination of monitoring the disease. CA724, CA242 and SCC can be as adverse prognostic indicators for cardia, stomach,esophageal malignant tumors, respectively.

Objective To study the differential diagnostic value of interferon-γ inducible protein 10 (IP-10),macrophage inflammatory protein-1 α (MIP-1 α) and monocyte chemoattractant-1 (MCP-1) level in the tuberculous,malignant pleural effusion.Methods Enzyme-linked immunosorbent assay was used to detect the level of IP-10,MIP-1 α and MCP-1 in tuberculous pleural fluid (tuberculous pleural fluid group,43 cases) and malignant pleural fluid (malignant pleural fluid group,45 cases).The level of IP-10,MIP-1 α and MCP-1 and the significance were analyzed by ROC curve.Results The level of IP-10,MIP-1 α and MCP-1 were significantly higher in tuberculous pleural fluid group than those in malignant pleural fluid group,and there were significant differences(t =4.931,3.106,2.385 ; P =0.000,0.004,0.041).ROC curve analysis showed that the critical value of IP-10,MIP-1 α and MCP-1 in diagnosis of pleural effusion was respectively 1 589.73,213.50,1 452.63 ng/L.The sensitivity and specificity of IP-10,MIP-1 α and MCP-1 in pleural fluid were 68.8％,81.3％,87.5％ and 87.5％,68.8％,56.3％,respectively.Conclusion The level of IP-10,MIP-1 α and MCP-1 in tuberculous and malignant pleural fluid are significant for the early diagnosis and differential diagnosis.

Objective: To explore the mechanisms of acupuncture in improving osteoporosis. Methods: In this study, the expression of OPG and BMP-2 proteins of femur in SAMP6 control group, SAMP6 acupuncture group, SAMP6 sham-acupuncture group and SAMR1 control group were determined by Western Blot technique. Results: Compared with SAMR1 control group, the expression of OPG and BMP-2 proteins of the femur in SAMP6 control mice decreased obviously. Acupuncture could stimulate the expression of OPG and BMP-2 and no differences were detected between the SAMR1 control group and SAMP6 acupuncture group. Conclusion: Osteoporosis observed in SAMP6 was correlated with low expression of OPG and BMP-2. After acupuncture treatment, both of them showed increased expression, it suggested that the curative effects of acupuncture on osteoporosis were partly due to stimulating skeleton formation, inducing the production of some local factors of bone metabolism and decreasing the bone turnover rate.