Objective To investigate the awareness rate of health knowledge,health behavior formation rate and psychological state before and after retirement intervention,analyse comprehensive psychological intervention and general psychological intervention to health behavior formation and improve the negative emotion in retired population.Methods By using cohort study method,206 retired people were randomly divided into comprehensive psychological intervention group (experimental group) and only given general psychological intervention group (control group),and each group included 103 cases.Comprehensive psychological intervention group were given health behavior and cognitive intervention,psychological intervention,psychological intervention in general family and social family care intervention,and the control group was given healthy behavior and cognitive intervention,psychological intervention.The staff were treated within 1 weeks of first assessment,2 weeks after the second assessment,4 weeks after the final.Health behavior was surveyed by Ministry of finance,the Ministry of health on public health subsidies special funds notice in city community health education pilot project in the questionnaire.Health status and quality of life were assessed with the family APGAR index (APGAR).Mental state evaluation and assessment of the treatment effect by using Hamilton Depression Scale (HAMD),MBI index method was assessed with activities of daily living.The effect of the two groups were compared before and after the intervention of health knowledge rate and formation rate of different intervention methods and psychological state of health behavior.Results The results showed that there were no significant differences between experimental group and control group in age,sex,level of education and other social,and there was no significant difference in HAMD,MBI and APGAR scores at baseline.Through the implementation of the comprehensive of psychological intervention,subjects have varying degrees of improving the awareness rate of health knowledge,and the intervention group was significantly higher than the control group (P ＜ 0.05).The two group of healthy behavior of subjects in the chopping board monitoring,physical fitness,blood pressure to separate raw and cooked on such projects had different degrees of improvement,at the same time,although no intervention,in the monitoring of blood pressure.The control group increased compared with the baseline significantly (P＜ 0.05).Intervention group had significant difference in APGAR score increased (P＜ 0.05).HAMD score and MBI score decreased and the differences were statistically significant (P＜ 0.01),while the control group had no significant difference before and after intervention.Conclusion Comprehensive psychological intervention can significantly improve the rate of formation from the awareness rate of health knowledge and health behavior of retirees,and improve the state of negative emotions from retiree.

Objective To study the effects of soybean isoflavone(SI) on lipid peroxidation and liver ultrastructure in ovariectomized rats. Methods Seventy female Sprague-Dawley(SD) rats were randomly divided into 7 groups according to the levels of total cholesterol(TC) in serum: hyper-lipoid group,estrogen group,low-dose SI group,middle-dose SI group,high-dose SI group,sham group and normal control group.After bilateral ovaries were extirpated except sham and normal control groups for a week,the estrogen,different doses of SI or deionized water were fed with intragastric administration for 12 weeks.The rat body weight was weighted once per week and blood samples were collected at the time of ovariectomization,the 4th and 8th weeks after administeration at the time of being killed.The serum TC,triglyceride(TG),high or low density lipoprotein-cholesterol(HDL-C,LDL-C),lipoprotein(a) and antioxygen enzyme were assayed. Results LDL-C levels in SI intervention groups were significantly lower than that in hyper-lipoid groups but higher than that in normal control group;the levels of lipoprotein(a) were changed;there was almost no effect on HDL-C in serum.SI could retain integrity and ultrastructure of hepatocyte;there was similar structure in high dose SI group and normal control group.An obvious damage was detected in hyper-lipoid group.Conclusion SI can improve lipoprotein(a) and lipid peroxidation in ovariectomized rats.A continuous intervention with high-SI can reduce LDL-C to normal lever and retain integrity and ultrastructure of hepatocyte.

Objective: To investigate the mechanism of apoptosis of CD8⁺T lymphocyte in peripheral blood of patients with hepatocellular carcinoma (HCC). Methods: The proportion and apoptosis of peripheral blood CD8⁺T lymphocytes in 30 healthy controls, 30 patients with cirrhosis and 60 HCC patients were detected by Flow cytometry, and the expression of Fas on the surface of CD8⁺T lymphocytes was reported. The differences between groups were compared using independent sample t-test, and data of variance were tested with Mann-Whitney U non-parametric test, P < 0.05 was considered statistically significant. Results: The proportion of CD8⁺T lymphocytes in peripheral blood of patients with HCC was 26.4% ± 9.2%, higher than that of 24.5% ± 7.1% in cirrhosis (t = 0.783, P = 0.489), and and healthy control 19.7% ± 4.7% (t = 2.920, P = 0.004). The proportion of apoptotic CD8⁺T lymphocytes in peripheral blood of HCC patients was 25.3% ± 6.5%, of the total CD8⁺T lymphocytes, which was significantly higher than that of healthy controls 12.1%±6.5% (t = 7.555, P < 0.001) and cirrhotic 13.6% ± 5.8% (t = 5.213, P < 0.001), the differences were statistically significant. The proportion of Fas⁺CD8⁺T lymphocytes in the HCC group was 62.2% ± 18.5%, higher than that in the healthy control group 42.6%±16.5% (t = 4.127, P < 0.001) and 46.1% ± 14.5% (t = 2.561, P < 0.01)of the cirrhosis group, the differences were statistically significant. Fas expression was positively correlated with the apoptosis of CD8⁺T lymphocytes (r ² = 0.113, P < 0.05). Conclusion The proportion of CD8⁺T lymphocytes in peripheral blood of patients with HCC is higher than that of healthy controls, but the proportion of CD8⁺T lymphocyte apoptosis based on Fas/FasL pathway increased, which may be an important mechanism for tumor cell immune escape.