Objective To study the application value of breast gland needle aspiration pathology on the early diagnosis of breast cancer. Methods 1324 cases of breast swelling, tumor and spilling-over patients were diagnosed and differentiation diagnosed needle aspiration pathology in position using disposable plastic injector of 5-10 volume. And it was compared with clinical and pathology tissue, CT, super-sound , breast scanning, mini-nucleus ,tuberculosis antibody ,CA153 etc. Results Breast gland needle aspirate pathology has important application value not only on diagnosis breast diseases, but also in the early diagnosis and differentiation diagnosis of breast cancer. It has especial instructive significance in differentiating whether breast proliferation and glandular fibro adenoma transfer into cancer. Puncture success rate is high up to 100%,early diagnosis rate is 16.9%,the whole diagnosis correctness is 98.6%,which is highest historically. Conclusions Breast needle aspirate pathology has the merits of little injury, being simple and quick, safe and sound, economic and practical correct results. All items of technology index are evidently higher than traditional diagnosis methods. It can’t be replaced by any other methods by now and it has high spreading value.