Objective To observe the effect of Chinese herbal compound Tangbikang on the level of plasma β-EP of diabetic peripheral neuropathy rats, and to discuss its mechanism of neuroprotection and abirritation. Methods STZ and feeding high fat forage induced diabetic rats were randomly divided into diabetic model group, Mecobalamin group, Tangbikang high dose group, Tangbikang medium dose group, Tangbikang low dose group, with 10 normal rats as control. The control group and model group were daily gavaged with distilled water, Tangbikang groups were given Tangbikang with different dose. Mecobalamin was used as the control medicine. Body weight and FBG was detected every 4 weeks, rats’ unilateral sciatic nerve were taken out at the 16th week. Pathological variation of sciatic nerve was presented by HE staining, and radioimmunoassay method was used to detect the levels of plasma β-EP of diabetic peripheral neuropathy of rats. Results The weight and FBG had obviously changed in the 16th week. Compared with the model group, pathological injury of diabetic peripheral neuropathy rats in the Tangbikang groups were significantly improved. The level ofβ-EP of model group was significantly decreased (P<0.01) compared with normal group. Tangbikang medium and high dose group significantly increased the level of plasma β-EP (P<0.01). Conclusion Chinese herbal compound Tangbikang could reduce the fasting blood glucose, protect nervous system and promote the level of plasmaβ-EP of diabetic peripheral neuropathy rats.

BACKGROUND:Studies have found that both bone marrow mesenchymal stem cels and umbilical cord mesenchymal stem cels have therapeutic effects on liver failure, but little is reported on adipose-derived mesenchymal stem cel transplantation for treatment of liver failure.
OBJECTIVE:To discuss the therapeutic effect of adipose-derived mesenchymal stem cel transplantation on rat liver failure.
METHODS: Forty-five Sprague-Dawley rats were equaly randomized into transplantation, model and control group. Except the control group, animal models of liver failure were made by intraperitoneal injection of D-galactosaminein in the transplantation and model groups. One week after modeling, rats in the transplantation group were given 1 mL of adipose-derived mesenchymal stem celsvia the tail vein.
RESULTS AND CONCLUSION:Adipose-derived mesenchymal stem cell transplantation successfully upregulated alanine aminotransferase and aspartate aminotransferase levels, and moreover, neatly arranged liver cells, reduced inflammatory cells and intact hepatic lobule were shown on B ultrasound after cell transplantation. All these findings indicate that adipose-derived mesenchymal stem cell transplantation has therapeutic effects on rat liver failure by improving rat’s liver function and pathological changes.