The clinical manifestation and laboratory features of juvenile hyaline fibromatosis(JHF)are reposed in a 5-year-old girl.Clinical symptoms began at 1 year of age,which was characterized by multiple cutaneous nodules and gingival hyperplasia with normal intelligence.Histopathologic examination of a skin biopsy specimen showed that the epidermis was normal,the nodules were composed of spindle-shaped fibroblasts embedded in homogeneous,non-fibrous,eosinophilic substance,and normal tissue was disrupted.Moreover,the fibroblasts showed transparent cytoplasm arranged in an obscure bundle-like pattern.No cell atypia or necrosis was observed.PCR amplification and sequencing of the capillary morphogenesis gene-2(CMG2)revealed a homozygous splicing mutation(IVS 14+1G→T)in the patient,while both of her parents were hetemzygous for the mutation.JHF is inherited in this family in an autosomal recessive manner.

Objective To explore the efficacy of the case and scenario teaching method in the neurosurgery practice teaching.Methods By cluster sampling,153 nursing students underwent rotary practice in neurosurgery department in People's Hospital of Three Gorges University from September 2011 to July 2012 were trained with the case and scenario teaching method.Scenario simulation training combined with case playback teaching was used in practice teaching.At the end of the internship,nursing students' satisfaction was investigated and their operational capacity was evaluated.Self-designed questionnaire was adopted to evaluate satisfaction.Operational capacity evaluation scores of nursing students were divided into several parts:basic operation examination (30％),specialized operation (30％),professional theory (30％) and teacher evaluation (10％).Data were analyzed using Excel spreadsheet for entry.Countif and Count function formula were used to calculate eligibility and the passing rate.Results Nursing students had high evaluation on case playback teaching methods.By this method,153(100％) nursing students considered that the learning initiative was improved.At the end of the internship,passing rates of basic operation examination,specialized operation,professional theory were 98.69％ (151/153),96.73 ％ (148/153) and 100％ while the total passing rate was 100％.Conclusion The case and scenario teaching method could develop the study positivity and practice ability of nursing students and can enhance the internship efficacy.

Objective To observe the influence of Maixuekang therapy on hematoma absorption,cerebral edema and neural function in patients with acute intracerebral hemorrhage within 3 weeks.Methods A total of 89 patients with acute intracerebral hemorrhage were divided into control group (45 cases) and treatment group (44 cases) by random digits table method.The two groups were implemented routine therapy and individualized rehabilitation treatment.Furthermore,the patients in treatment group were treated with Maixuekang (1.0 g,thrice a day for 3 weeks),if reexamination result of cerebral CT showed no expanding hematoma after 24 h from the onset of intracerebral hemorrhage.Cerebral hematoma and edema volume was measured by brain CT before therapy and on the 7th,14th and 21st day after therapy,and neurologic impairment was scored at the same time points by neurological impairment scale(NIS).The results were analyzed with statistics.Results The cerebral hematoma volume,edema volume and NIS before therapy,on the 7th,14th and 21st day after therapy in two groups respectively was (22.4 ± 8.4) ml,(9.5 ±2.6) ml,(25.4 ±3.8) scores,(21.7 ±7.6) ml,(19.9 ±7.1) ml,(25.3 ±3.4) scores,(18.9 ±6.7) ml,(18.3 ± 5.7) ml,(23.8 ± 3.0) scores,(15.3 ± 5.4) ml,(14.5 ± 4.8) ml,(21.7 ± 2.5) scores in control group; (22.3 ± 8.6) ml,(9.4 ± 2.5) ml,(25.3 ±3.6) scores,(19.6 ± 7.0) ml,(17.3 ± 6.4) ml,(23.9 ± 3.2) scores,(15.5 ±5.9) ml,(15.1 ±4.5) ml,(20.5 ±2.8) scores,(11.2 ±4.5) ml,(10.7 ±3.3) ml,(17.4 ± 2.1) scores in treatment group.The cerebral hematoma volume and edema volume was significantly smaller in treatment group than that in control group after therapy (P ＜ 0.05 or ＜ 0.01),but did not show significant difference compared with that before therapy (P ＞ 0.05).Conclusion Maixuekang therapy can significantly reduce cerebral edema and contribute to hematoma absorption and nerve functional recovery in patients with acute intracerebral hemorrhage.

AIM: To obtain differentially expressed cDNA fragments in the cerebellum of rats and screen unknown expressed sequence tag (EST). METHODS: Suppression subtractive hybridization (SSH) was carried out, in which the cDNA fragments of cerebellum were taken as "tester" and correspondingly that of cerebrum and brain stem as "driver". The homogeneous sequences between the tester and driver were excluded and the rare sequences in cerebellum were enriched by SSH. The differentially expressed cDNA fragments were further cloned for the construction of subtracted cDNA libraries and sequencing. RESULTS: 32 clones were selected and 34 cDNA fragments were sequenced. 8 of 32 were proved to be true positive with reverse Northern assay, 13 of 34 fragments were identified to be new cDNA fragment and given the gene sequence numbers by GenBank (AW288461-AW288474). CONCLUSION: SSH is very useful method for screening differentially expressed genes. Our data may be helpful to understand the molecular mechanism of brain function.

Objective To screen the differentially expressed genes in optic tecta of pigeon with monooccular deprivation. Methods Using suppression subtractive hybridization technique,the differentially expressed cDNA library for the left optic tecta of pigeons was constructed;recombinant clones were sequenced randomly and further proved to be the true positive by reverse Northern blot analysis. Results 150 of EST (expressed sequence tags) were sequenced and 16 of them proved true positive,which included those homologous to known genes and those having no matches.138 of ESTs were assigned Accession Number by Genbank.Conclusion\ Our data provided to be the useful clues for studying the development of visual system and the molecular construction of vision center system.

Objective To investigate the effect of genistein on the expression of Bax and Bcl-2 protein in PC12 cells transfected with mutant APP695 Type.Methods PC12 cells were transfected with pIRES2-EGFP plasmid or pIRES2-EGFP/APP695MT expression plasmid,and then divided into normal control group,Unladen group,APP695 transfected group,GST treatment group(5 μmol/L,15 μmol/L).The Bax and Bcl-2 protein expression in PC12 cells was measured by Immunocytochemistry SABC and Western-blot.Results Immunocyto-chemistry SABC showed that:compared with the normal control group,the expression of bax protein in APP695 transfected group was strongly increased((78.02 ± 1.26) ％,P＜0.01)) and the expression of bcl-2 protein was very weak oppositely ((15.40 ± 0.72) ％,P＜0.01)).Compared with APP695 transfected group,the expression of bax protein in cells of 15umol/L GST treatment group was significantly decreased((22.35 ± 0.49) ％,P＜0.01))and the expression of bcl-2 protein in these cells was strongly increased ((68.11 ± 0.24) ％,P ＜0.01)).Western-Blot showed that:compared with the normal control group,the expression of bax protein in APP695 transfected group was markedly increased and the expression of Bcl-2 protein was significantly decreased.Compared with the APP695 transfected group,the expression of Bax protein was markedly decreased and the expression of Bcl-2 protein was significantly increased in the cells of the GST treatment groups.Conclusion GST can reduce the expression of bax protein and the increased expression of Bcl-2 protein has protective effect on PC12 cells transfected with APP696MT.

Objective To construct lentiviral expression vector of rat IP3R1 gene,and identify its silencing effect by using PC12 cell lines. Methods Oligo DNA sequences of 4 pairs of miRNA,named as miRNA1,miRNA2,miRNA3 and miRNA4,were designed according to IP3R1 gene sequence (GenBank:NM_001007235). The single strand of oligo DNA was annealed to form double strand DNA,and then connected with the empty plasmid pcDNA TM 6.2-GW/EmGFP-miR. By using gateway technology,the expression vector pcDNA TM 6.2-GW/EmGFP-miR-IP3R1 was linked into lentiviral destination vector pLenti6/V5-DEST to form the lentiviral expression vector pLenti6/V5-DEST-IP3R1,then it was transformed into infectious lentiviral particles and to infect PC12 cell lines. Silencing effect of gene IP3R1 was detected by Real-time PCR and Western blot. Results The sequence of expression vector pcDNA TM 6.2-GW/EmGFP-miR-IP3R1 was proved correct using sequencing method. After the transfection of letivirus vector pLenti6/V5-DEST-IP3R1 into PC12 cell lines,the IP3R1 mRNA and protein level were down-regulated 48h later,of which miRNA2 and miRNA3 sequence showed the best silencing effect,and the expression of IP3R1 in the blank control and negative control showed no significant changes. Conclusions Lentiviral expression vector pLenti6/V5-DEST-IP3R1 was constructed successfully. pLenti6/V5-DEST-IP3R1 may render the IP3R1 expression in PC12 cell lines down-regulated,and it provides a foundation for studying the function of calcium release channel IP3R1.

BACKGROUND: It is the most effective and economical method to reduce the incidence of stroke by conducting the health education concerning the etiological factors or risk factors and therapeutic knowledge of hypertension, diabetes mellitus and hyperlipidemia among the public and improving their knowledge level about the prevention and treatment of stroke.OBJECTIVE: To investigate the cognition of the knowledge concerning the prevention and treatment of stroke among the public in Dongguan area.DESIGN: A cluster sampling and simple randomized sampling survey.SETTINGS: Department of Neurology, the Fifth Affiliated Hospital of Sun Yat-sen University and the Department of Neurology, Affiliated Tungwah Hospital of Sun Yat-sen University.PARTICIPANTS: Between March and June in 2001, totally 2 500 cases were randomly selected by means of cluster sampling combined with simple randomized sampling from the residents and migrant populations no younger than 17 years old in the government organization, enterprises, factories of various forms, Dongguan University of Technology and senior middle schools of urban and rural Dongguan city and the administrative villages of the rural areas of Dongguan city in Guangdong province before March 2003.METHODS: The inventory was modified according to relevant literatures,including demographic data, general knowledge of stroke, and knowledge about the prevention, identification and treatment of stroke, and the pathway to obtain knowledge about the prevention and treatment of stroke.Each item for the knowledge about the prevention and treatment of stroke was marked as 1-4.5 points, and the total score was 60. There was no mark for the pathway to obtain knowledge about the prevention and treatment of stroke. One unit was investigated each time, and 300-600 questionnaires were sent out, 500 questionnaires were randomly handed out to the healthy physical examinees in the Tungwah Hospital.MAIN OUTCOME MEASURES: The correct rate of answers to the questionnaire, the total score of each kind of knowledge about the prevention and treatment of stroke, the total score of each kind of knowledge in the subgroups, and the pathways to obtain knowledge about the prevention and treatment of stroke were mainly observed.RESULTS: Totally 2 320 complete and effective questionnaires were collected back. ① The average correct rate of answers to all the questions was 49.80%, and those of knowledges about identification and treatment were lower, the total score was ＜ 60% in 48.20% of the subjects. ② The level for each knowledge about the prevention and treatment of stroke was lower in males than in females (P ＜ 0.05), but higher in the middle-age and old groups than in the young group (P＜0.05), and it was decreased from various civil servants, free agents, workers, farmers and students in order (P＜0.05), and there was significant difference between students and free agents (P＜0.01); it was also decreased in order from higher educational level to middle and lower ones in order (P＜0.05); it was also higher in the subjects with stroke history than in those without (P ＜ 0.05). ③) After the subjects were subdivided according to gender, age, profession and educational level, the pathways to obtain knowledge about the prevention and treatment of stroke in order were reading newspapers and magazines,watching TV, reading handbooks about science popularization, influence from relatives and friends, promotion and education of medical staff, and the last one was the least in all the groups.CONCLUSION: The level for the knowledge about the prevention and treatment of stroke was lower among the public in Dongguan area during the investigation, especially the knowledges about identification and treatment, so proper measures should be adopted to further develop education on the knowledge about the prevention and treatment of stroke.

Objective To detect the expression of IL-17 in renal transplant recipients by Luminex and evaluate the relationship between the level of serum IL 17 and early acute renal allograft rejection.Methods 38 kidney transplant recipients and healthy controls (HC,healthy volunteers,n =20) were selected in this study from January 2009 to October 2011.All patients were divided into two groups according to their allograft outcome as acute rejection group (ARG,n-18) and non-rejection group (NRG,n=20).The expression of serum IL-17 was detected by Luminex technique in two groups of kidney transplant recipients and HC.To evaluate the correlation between the level of serum IL-17 and early acute renal allograft rejection.Results The mean level of IL-17 in all renal transplant recipients 1.3 ± 1.9 pg/ml at the pre-transplantation was significantly lower than that in HC (6.9± 8.5 pg/ml,t=3.968,P＜0.001).The results highlighted that the level of serum IL-17 in ARG 3.4±5.8 pg/ml was significantly higher than that in NRG (0.5±0.4 pg/ml) on the 7th days post Kidney transplantation (post KTx,t=2.242,P =0.031).Kaplan-Meier survival analysis showed that the probability of rejectionfree survival in the higher levels group of IL-17 on the 7th days post-KTx was significant lower than that in the lower levels group (P＜0.001).The results of receiver operating characteristic curve showed that the sensitivity and specificity of the combined IL-17 to predict acute rejection were 66.67％ and 100％,respectively.Conclusion The serum IL-17 levels in renal transplant recipients on the 4th and 7th post-KTx days can predict early renal transplant rejection.

Quality of medical records is key to hospital′s total quality management, while the establishment and improvement of real-time monitoring feedback system is of great significance to the improvement of the quality of medical records. The authors explored real-time quality control of electronic medical records through artificial intelligence data processing and machine learning. The quality control covers timeliness and completeness in writing the medical records, the consistency and logic of the content, the identification of typos and the quality control of the medical records etc. Its practice showed that the defect rate of medical records has decreased significantly and the quality control management efficiency has greatly improved.