Objective To investigate curative effect in children with bronchial asthma by two treatment methods. Methods 100 children with bronchial asthma,according to the different treatment were divided into treatment group 50 cases and control group(50 cases). The first seconds vital capacity(FEV1),peak expiratory flow (PEF) ,vital capacity 25% of the instantaneous velocity (V75), vital capacity 50% of the instantaneous velocity (V50) ,vital capacity 75% of the instantaneous velocity(V25) and their percentage the percentage of predicted value were observed. Results Total effective of 43 cases(86.0%) in treatment group after treatment 4 weeks were higher than that in control group of 28 cases (56. 5 %) (x~2 = 3. 987, P ＜ 0.05); The percentage of FEV1 and PEF in two groups after treatment 12 weeks were significantly higher than that before treatment(x~2 = 4. 01,4. 21,4. 31,4. 08, all P ＜ 0.05), but it between the two groups was not statistically significant (x~2 = 2. 31,2. 41, all P ＞ 0.05); The pulmonary function of V75 、V50、V25 in two groups after treatment 12 weeks reached projected value of more than 90% ;The pulmonary function of V75 、V50、V25 after treatment compared with those before treatment were improved significantly (x~2 =4.285,4.234,4.311,4.278,all P＜0.05). Conclusion The curative effect of bronchial asthma were confirmed in two methods,but treatment group were better than the control group.

Objective To investigate the molecular mechanisms that are responsible for anti-inflammatory effect of usnic acid (UA), the effects of UA from usnea longissm on tumor necrosis factor-α(TNF-α) and nitric oxide (NO) production in peritoneal macrophages has been examined. Methods The different concentrations of UA were added to peritoneal macrophages. The TNF-α and NO production in peritoneal macrophages were examined with mouse TNF-α ELISA kit and NO content by measuring the amount of nitrite (NO-2μmol/L) formed in the medium using Griess reaction. The activity of inducible nitric oxide synthase (i-NOS) was determined using i-NOS detection kit and the TNF-α mRNA expression was tested by reverse transcriptase polymerase chain reaction (RT-PCR). Results UA decreased the TNF-α and NO level in LPS-stimulated peritoneal macrophages in dose-dependent manner, the IC50 values were 12.8μmol/L and 5.7μmol/L respectively. RT-PCR analysis indicated that UA could inhibit TNF-α mRNA expression; the activity analysis of i-NOS indicated that UA could inhibit the activity of i-NOS. Conclusion UA could inhibit the TNF-α and NO production in peritoneal macrophages, it may be associated with the anti-inflammatory activity of UA.

Objective To investigate the clinical effects of Kangfuxin Solution used together with Baofukangshuan on chronic cervicitis after having been processed cervical LEEP circumcision.Methods Retrospective analysis was made among 176 out-patients diagnosed of chronic cervicitis from November 2007 to January 2009.Of all these patients,90 patients were treated with Kangfuxin Solution and Baofukangshuan after LEEP circumcision(the treatment group),and 86 patients were treated with simple cervical LEEP circumcision(the control group).The wound healing,vaginal discharge,bleeding volume,and side effects were observed in both groups.Results The method in the treatment group significantly reduced the amount of vaginal discharge and the duration,decreased the amount of bleeding and the duration;excluded wound infection and adverse reactions,and facilitated cervical wound recovery.The curative rate(98.89%)in the treatment group was significantly higher as compared to the control group(86.05%)after 8 weeks of the treatment.Conclusion Cervical LEEP circumcision surgery supplemented by Kangfuxin solution and Baofukangshuan therapy has better efficiency than treated by simple cervical LEEP circumcision.

Objective To assess the therapeutic effect of esomerphrazole in treating patients with gastroesophageal reflux disease (GRED) between Han and Inner Mongolia populations.Methods Those who underwent endoscopic examination and had reflux disease questionnaire (RDQ) >12 from March 2006 to March 2008 were selected. The patients were divided into Han group and Inner Mongolia group with 120 each. All patients were received esomerphrazole 20 mg daily for 6 weeks. The patients were evaluated by RDQ questionnaire at 2nd, 4th and 6th week, and were reexamined by gastroscopy at 6th week. Results After two weeks, effective rate of 50% was achieved in both groups, but it was higher in Han group than in Inner Mongolia group at 4th and 6th week. After 6 weeks, the curative rate of RE was higher in Han group than in Inner Mongolia group. Conclusion The different nations ancl habits result in the different efficacy of esomerphrazole, which may be improved by prolonging time of medication and changing habits.

AIM:To investigate the protective effect of lycopene on primary mouse cerebrocortical neurons ex -posed to tert-butyl hydroperoxide ( t-BHP) and its mechanisms of in vitro.METHODS:Primary cerebrocortical neurons of newborn C57 mice were extracted and divided into normal group , t-BHP group, lycopene +t-BHP group and lycopene group.The neuronal damage was induced by t-BHP exposure for 24 h, and the cell viability was examined by MTT assay . ROS content was measured by flow cytometry , and the protein levels of Bax , Bcl-2, caspase-3, cleaved caspase-3 and cyto-chrome C were examined by Western blot .RESULTS:The primary mouse cortical neurons expressed MAP-2 protein.Ly-copene at concentration of 4μmol/L reversed the decrease in cell viability .Flow cytometry revealed that lycopene treatment attenuated ROS content under the condition of t-BHP exposure.In addition, the protein level of Bcl-2 was increased, and the expression of Bax , cleaved caspase-3 and cytochrome-C was suppressed in lycopene +t-BHP group.CONCLUSION:The protective effect of lycopene on cortical neurons with t-BHP-induced injury may be involved in the mechanism of neuro-nal antioxidative response by down-regulating caspase-3 and Bax/Bcl-2 through the mitochondrial apoptotic pathway .

Objective To determine the value of detection of H ras oncogene mutation in urine exfoliated cells as clinical indicator of tumor presence, recurrence and stage.Methods Point mutation at codon 12 of H ras gene was assayed by polymerase chain reaction followed by analysis of single strand conformation polymorphism in urine exfoliated cells from 48 patients with transitional cell carcinoma before operation and 28 patients with non urothelial cancer or normal individuals. The mutation was further confirmed by dideoxy mediated chain termination method of DNA sequencing. Cytology analysis was carried out simultaneously. Bladder tumor specimens were obtained from 48 patients during operation, and histologically elevated for tumor content and grading.Results 48%(23 of 48) of the patients were detected by their aberrant band in SSCP. All aberrant bands displayed a mutant H ras sequence, where 15% (7 of 48) of the patients displayed, apositive cytological analysis. Analysis of abnormalities with tumor stage revealed that the greater detection of high pathological stage (Ⅲ Ⅳ) compared with low stage (Ⅰ Ⅱ) was related to the recurrence of transitional cell carcinoma.Conclusion Our results suggest that the detection of H ras mutations may be of clinical value in the detection of TCC.

OBJECTIVETo study the relationship between glutathione S transferase M1(GST mu) gene deletion and leukemia in workers exposed to benzene.

METHODSA matched population-based case-control survey with multivariate Logistic regression analysis was conducted in this study.

RESULTSIn the population of 34 patients and their matched controls, the absence of the GST mu genotype conferred odds ratio of 3.6. It suggested that GST mu was an important determinant of heterogeneity in individual susceptibility to leukemia associated with exposure to benzene. The single-variance analysis indicated that these markedly significant factors were GST mu gene deletion, GST mu isoenzyme activity, duration of exposure, GST isoenzyme activity, smoking quantity and average concentration of benzene in workshop air. The multivariate analysis indicated that these markedly significant factors were GST mu gene deletion, duration of exposure to benzene and GST mu isoenzyme activity.

CONCLUSIONGST mu gene deletion may be associated with increased risk of leukemia in workers exposed to benzene and is one of genetically determined factors.

Benzene ; toxicity ; Case-Control Studies ; Gene Deletion ; Glutathione Transferase ; genetics ; Humans ; Leukemia ; enzymology ; etiology ; genetics ; Logistic Models ; Multivariate Analysis ; Occupational Exposure ; adverse effects

OBJECTIVETo study the tissue culture and plant regeneration technologies and optimizing propagation system in vitro of Rhodiola henryi.

METHODOrthogonal experiment designs were used in the study of Rh. henryi callus induction, shoot formation and rooting, and the data were analyzed by range analysis and variance analysis.

RESULTThe optimal media to induce multiple callus from leaves were MS supplemented with 2,4-D 1.5 mg x L(-1) and 6-BA 0.5 mg x L the effect of the three factors was in sequence of explants > 2,4-D > 6-BA; The optimal media to induce multiple buds from stems were MS supplemented with 6-BA 1.5 mg x L\/1-1 NAA >6-BA; Plantlets were rooted on 1/2MS supplemented with IBA 1.0 mg x L-1, and rooting rate reached to 90% or more and transplant survival rate of plantlet reached 98% or more.

CONCLUSIONAn efficient system for tissue culture and plant regeneration of Rh. henryi was initially established.

Culture Media ; pharmacology ; Regeneration ; drug effects ; Rhodiola ; drug effects ; physiology ; Tissue Culture Techniques ; methods ; Wound Healing ; drug effects

Objective:To explore the teaching effect of Ideological and Political Education+MOOC+BOPPPS in the teaching of Medical Immunology and Pathogenic Biology.Methods:Three majors of nursing,pharmacy and midwifery of grade 2021 in a higher vocational college were selected.One class was randomly select from each major as the control group,and received PBL+CBS teaching mode.One class was randomly selected from each major as the experimental group,and received the teaching mode of Ideological and Political Education+MOOC+BOPPPS.The teaching effect was evaluated by theoretical results,questionnaire survey,and"practical ideological politics".Results:The theoretical examination score in the experimental group was significantly higher than that in the control group(P＜0.05).The experimental group had a higher satisfaction with the teaching model than the control group.The score of professional commitment in the experimental group was significantly higher than that in the control group(P＜0.01),and the learning burnout score in the experimental group was lower than that in the control group(P＜0.05).The number of students in the experimental group participating in"practical ideological politics"was higher than that in the control group.Conclusion:The teaching mode of Ideological and Political Education+MOOC+BOPPPS helps to improve the educational effect of Medical Immunology and Pathogenic Biology,and provides a basis for the teaching of Ideological and Political Education in medical professional courses in higher vocational medical colleges.

Objective:To explore the effect of pathway nursing under multidisciplinary collaboration on self-care ability and rehabilitation effect in patients with acute cerebral infarction.Methods:Using the convenient sampling method, a total of 294 patients with acute cerebral infarction who were admitted to the Department of Neurology in the Fengcheng Hospital, Fengxian District, Shanghai from January 2018 to December 2019 were selected as the research objects. A total of 147 patients with acute cerebral infarction admitted from January to December 2019 were included in the experimental group, while 147 patients with acute cerebral infarction admitted from January to December 2018 were included in the control group. The experimental group adopted pathway nursing under multidisciplinary collaboration, while the control group adopted neurology routine nursing. Scores of Exercise of Self-Care Agency (ESCA) , National Institutes of Health Stroke Scale (NIHSS) , Fugl-Meyer Assessment (FMA) , Barthel Index and nursing satisfaction of patients were compared between the two groups. Finally, 147 patients in the experimental group and 145 patients in the control group completed the experiment.Results:The scores of self-concept, self-responsibility, health knowledge level and self-care skills in ESCA of patients in the experimental group at discharge were respectively (25.36±4.86) , (20.43±3.21) , (53.37±5.89) and (40.02±3.78) and the nursing satisfaction score was (97.26±2.52) , which were higher than (21.48±4.33) , (16.24±3.25) , (46.44±5.47) , (30.08±3.81) and (90.65±3.16) in the control group, and the differences were statistically significant ( P<0.01) . The NIHSS score of the experimental group 60 days after discharge was (3.7±2.2) , lower than (6.9±2.3) of the control group. And FMA and Barthel Index scores were respectively (79.3±9.2) and (78.7±14.5) , higher than (65.6±8.4) and (60.3±16.6) of the control group, and the differences were statistically significant ( P<0.01) . Conclusions:The implementation of pathway nursing under multidisciplinary collaboration for patients with acute cerebral infarction can enhance the consciousness of self-management, contribute to the recovery of neurological function and limb motor function and improve the ability of self-care and nursing satisfaction of patients.