Cyclin E is expressed starting from the middle G1 phase of the cell cycle,and is accumulated in the G1/S boundry.Cyclin E binds to and activates the cyclin-dependent kinase CDK2.Cyclin E-CDK2 complex initiates a cascade of events that leads to DNA replication by phosphorylating its substrates,such as Rb,CDC6,NPAT and P107,etc.Additionally,cyclin E plays an important role in the regulation of genomic stability,spindle-organizing structure and centrosome cycle.Cyclin E expression is trans-activated by members of the transcription factor E2F family and degrades via the ubiquitin-proteasome pathway.At the same time,it is also negatively regulated by the CIP/KIP proteins.Cyclin E highly expressed in the initiation and progression of different human cancers,such as breast cancers,lung cancers,leukemia,lymphomas and others.

General education is the quality education of higher education , which includes the humanities , so-cial science and natural science .It point out the present status of medical colleges and universities to carry out the general education of humanities and the existing problems which based on the current China 's higher medical colle-ges and universities to strengthen the urgency of the general education of humanities .It main including:the lack of humanistic concept of general education and human general curriculum is not reasonable ;the human general educa-tion hasn't dived into the medical professional education which lead to the students attention degree is not enough ;Human general education methods is single , practical link is weak .To solve these problems should be set up a med-ical humanities general education organization and management agencies , build reasonable medical humanities gen-eral course system , strengthen the construction of teaching staff of a curriculum , improve education curriculum teaching methods and many other countermeasures and suggestions .

Objective To analyze the relationship between the serum CysC,lipoprotein(a) [Lp(a)] and the urinary microalbumin/creatinine ratio in elderly type 2 dibetic (T2CM) patients.Methods A total of 102 elderly patients with T2CM who were treated in our hospital from December 2014 to December 2015 were selected.The patients were divided into diabetic nephropathy(DN) group(mALB≥20 μg/min,48 cases)and non-diabetic nephropathy(NDN) group (mALB<20 μg/min,54 cases) according the levels of urinary mALB,while 30 cases of healthy controls were selected from physical examination center.The biochemical indexes,CysC,Lp(a) and UACR were detected among all cases.The correlation between indexes and UACR was analyzed by Pearson correlation analysis,risk factors for UACR among DN patients were analyzed by Logistic regression analysis.Results There were significant differences in levels of low density lipoprotein cholesterol(LDL-C),Lp(a),urea,creatinine,fasting blood glucose(FBG),glycosylated hemoglobin(HbA1c),Cysc,and UACR among these groups (P<0.05).No correlation between CysC、Lp(a) and UACR was found in normal-control group and non-diabetic nephropathy group.In diabetic nephropathy group,there was a positive correlation between CysC,Lp(a) and UACR(r=0.658,P<0.01;r=0.525,P<0.05).The Logistic regression analysis showed that diabetes duration,CysC,Lp(a) were independent risk factors for UACR(P<0.05).Conclusion In patients with diabetic nephropathy,CysC,Lp(a) are positively correlated with UACR,and CysC is a sensitive index that reflect early renal damage in T2DM patients.Lp(a) level is one of the independent risk factors for UACR,which can reveal the kidney damage in DN patients.

The article describes immune disorder mechanisms of allergic rhinitis and the progress and clinical application status of the nonspecific immune therapy of allergic rhinitis, which can raise people's understanding about the nonspecific immune therapy of this disease.
Humans ; Immunotherapy ; methods ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; therapy

Objective To observe the therapeutic effect of ear-acupuncture therapy for intractable hiccups in the patients treated with endovascular embolization for bleeding from spontaneous rupture of liver cancer. Methods A total of 60 patients with intractable hiccups after endovascular embolization for bleeding from spontaneous rupture of liver cancer were randomly divided two groups using a random digit table, 30 in each group. The patients in the control group were treated breath holding combined with oral baclofen, and those in the observation group with ear-acupuncture therapy for 5 days, and then both groups were followed up for 1 week. The Karnofsky Performance Status Scale (KPS) was used for quality of life evaluation. The adverse events and recurrence during the treatment and the followed up were recorded. The therapeutic effect was evaluated. Results Total effective rate (100% vs. 76.7%; χ2=5.822, P=0.016)in the observation group was significantly higher than that in the control group. The rate of adverse events (6.7% vs. 33.3%; χ2=5.104, P=0.024)in the observation group were significantly lower than that in the control group. There was no significant difference in the KPS scores after treatment between the observation group and the control group (82.1 ± 12.6 vs. 76.5 ± 17.8; t=1.407, P=0.165). Conclusions Ear-acupuncture therapy may efficiently alleviate intractable hiccups in patients treated with endovascular embolization for bleeding from spontaneous rupture of liver cancer, and superior to breath holding combined with oral baclofen.

OBJECTIVE: To evaluate the effect and feasibility of coblation treatment for laryngopharynx hemangioma. METHOD: Retrospectively analyzed 24 cases with coblation treatment for laryngopharynx hemangioma in recent years. RESULT: Twenty-four patients were treated with coblation, follow-ups range 6 months to 24 months after surgry. Recurrence occurred in 2 months after surgry in one patient who was cured with coblation for second time. There was incomplete vocal cord paralysis in another patient because of wide range angeioma. CONCLUSION Coblation treatment for laryngopharynx hemangioma was an ideal way to less bleeding, less damage and more clearfield of vision.
Catheter Ablation ; Hemangioma ; surgery ; Humans ; Hypopharynx ; pathology ; surgery ; Laryngeal Neoplasms ; surgery ; Neoplasm Recurrence, Local ; Pharyngeal Neoplasms ; surgery ; Retrospective Studies ; Vocal Cord Paralysis

Objective To explore the tabular condition assessment methods in the role of early warning of children with severe hand, foot and mouth disease. Methods A total of 267 patients in observation group were evaluated by the disease assessment scale of hospitalized children with hand, foot and mouth disease and the risk assessment form of severe children with hand, foot and mouth disease to early indentify the severe and give intervention treatment. And the 243 patients in control group were evaluated by the empirical method. The incidence of severe cases and the incidence of complications were compared between two groups. Results The incidence of severe cases was 3.00%(8/267) in observation group and 17.70%(43/243) in control group, and there was significant difference between two groups, χ2=30.970,P<0.01. The incidence of total complications was 9.74%(26/267) in observation group and 32.51%(79/243) in control group, and there was significant difference between two groups, χ2=34.74,P<0.01. Conclusions The tabular condition evaluation method can early identify the children with severe hand, foot and mouth disease and give early intervention, reduce the incidence of severe and critical cases, and reduce the complications.

To explore the influence of low light on the synthesis of carotenoids, chlorophyll and the adaptability of transgenic plants with tomato Solanum lycopersicon L. GGPS2 gene, we constructed a vector containing a GGPS2 gene with green fluorescent protein (GFP) as report gene under the control of a cauliflower mosaic virus 35S promoter and introduced it into tobacco Nicotiana tabacum L. cv. Wisconsin 38 by Agrobacterium tumefaciens-mediated transformation. PCR analysis of the DNA from kanamycin resistant tobacco indicated that the transgenic tobacco containing the nptII gene, SlaGGPS2 gene and without contamination of Agrobacterium. We also detected the root tip of kanamycin resistant tobacco showing characteristic fluorescence. The contents of carotenoid, chlorophyll and photosynthesis of transgenic tobacco increased in comparison with wild tobacco after low light treatment. In addition, leaf mass per unit area, total dry weight, ratio of root to shoot in transgenic tobacco were all higher than that of the wild tobacco, which proved that the transgenic tobacco could increase the accumulation of biomass and promote it transport to root. The transgenic tobacco with SlaGGPS2 gene can increase the contents of carotenoid, chlorophyll, enhance the photosynthetic rate, promote the biomass accumulation and its distribution to root. Hence, the transgenic tobacco with SlaGGPS2 gene had increased low light tolerance and the SlaGGPS2 gene maybe can be used in other crops.
Agrobacterium tumefaciens ; Carotenoids ; analysis ; Chlorophyll ; analysis ; Gene Expression Regulation, Plant ; Genetic Vectors ; Light ; Lycopersicon esculentum ; genetics ; Photosynthesis ; Plants, Genetically Modified ; metabolism ; radiation effects ; Tobacco ; metabolism ; radiation effects

Objective To investigate the inductive role of traumatic microenvironment in dedifferentiation of epidermal cells and explore the potential role of Wnt signaling pathway in this biological process. Methods The sheets of human foreskin were digested overnight after removal of adipose tissue, and then the epidermis was separated from the dermis. The separated epidermis sheets were repeatedly adhered to type Ⅳ collagen and flushed to remove the epidermal stem cells. The obtained epidermis sheets were transplanted onto the full-thickness skin wounds on the back of BALB/c nude mice, five days after which the cell lineage was evaluated by immunohistochemistry and the expressions of Wnts and downstream components in the grafted epidermal sheets examined by RT-PCR and Western blot. Results The cells in the basal layer of full-thickness epidermal sheets were positive for CK19 and β1 integrin and negative for CK10. While the cells in uhrathin epidermal sheets treated with type Ⅳ collagen were fully positive for CK10. Five days after transplantation of the ultrathin epidermal sheets, cells negative for CK10 but positive for CK19 and β1 integrin emerged at the wound-neighboring side of the skin grafts. At the same time, the expressions of Wnt-10b, Wnt-4 and Wnt-7a mRNA were increased by about 3.1-fold, 2.2-fold and 1.4-fold independently after transplantation. Furthermore, the expressions of β-catenin and β-catenin target genes (cyclin D1 and c-myc) were elevated by about 3-fold, 1.5-fold and 2-fold respectively in the grafted epidermal sheets (P < 0.01). Conclusion Traumatic microenvironment can induce epidermal cell dedifferentiation, when the Wnt/β -catenin signaling pathway may play an important role.

A 56 years old female was admitted to our department with complaint of a painless cervical mass. Clinical feature:there was a painless mass above left lobe of thyroid gland, which was about 3.0 cm x 2.5 cm in size, and could move with swallowing action. B-mode ultrasound features: there was a solid mass in left lobe of thyroid gland, which was about 3.2 cm. Nodule was found in isthmus, accompanied with lymphadenovarix on the left neck possibly be MCA. fT3: 4.64 pmol/L, fT4:16.56 pmol/L,TSH:3.74 mIU/L, anti-TG:17.75 U/ml, anti-TPO:40.77 U/ml. Pathological result of the neoplasm: papillocarcinoma. Clinical diagnosis: papillary thyroid carcinoma.
Carcinoma ; diagnosis ; pathology ; Carcinoma, Papillary ; diagnosis ; pathology ; Deglutition ; Female ; Humans ; Middle Aged ; Neck ; pathology ; Parathyroid Glands ; pathology ; Recurrent Laryngeal Nerve ; pathology ; Thyroid Cancer, Papillary ; Thyroid Neoplasms ; diagnosis ; pathology