Objective To investigate the expression of matrix metalloproteinase-2 (MMP-2) mRNA in lungs of rabbits with acute lung injury (ALI) induced by endotoxin (ET) and the effects of meloxicam in its treatment, and to explore the protective mechanism of meloxicam. Methods Twenty four Japanese flap-eared white rabbits were randomly assigned to three groups: control group, ET-challenge group and meloxicam-treatment group. There were eight rabbits in each group. ALI model of rabbits was replicated with intravenous ET injection (700?g/kg weight), and meloxicam (2.5mg/kg weight) was intravenously given afber ET challenge in the treatment group. During the experiment, arterial blood gases, lung wet/dry (W/D), lung water content, the pathologic changes in lung, the expressions of MMP-2 mRNA in the lungs were determined. Results Compared with control group, oxygen saturation index (PaO_2/FiO_2) was significantly decreased and pulmonary edema, hemorrhage, extensive inflammatory cells infiltration were observed in ET group. The lung wet/dry (W/D), lung water content and the expressions of MMP-2 mRNA were significantly higher in ET group than those in control group. In meloxicam-treatment group, PaO_2/FiO_2 remained in normal range, and the lung wet/dry (W/D), the lung water content and the expressions of MMP-2 mRNA were significantly lower than those in ET group. The pathologic changes in lung tissues were not severe in group C. Conclusion The up-regulation of MMP-2 mRNA expression was observed in ALI animal model. Meloxicam could down-regulate MMP-2 mRNA expression, producing protective effects on ALI induced by ET in rabbits.

Objective To investigate the changes in interleukin-19(IL-19)in lungs of rabbits with acute lung injury(ALI)induced by endotoxin(ET),so as to study the mechanism of injury of ET to the lung and the protective mechanism of meloxicam.Methods Twenty four male Japanese flap-eared white rabbit was randomly assigned to three groups:control group,ET-treated group and treatment with meloxicam group.Rabbit ALI model was replicated with intravascular ET injection(700?g/kg),and meloxicam was intravenously injected(2.5mg/kg)for treatment group.The content of IL-19 was measured with ELSIA method and the changes in malondialdehyde(MDA)and superoxide dismutase(SOD)were determined in every group.Results IL-19 expression in ET challenged group was significantly higher than that in control group(P

Motion segment and extraction from continuous signals is the premise of surface electromyography (sEMG) analysis. For the problem that sEMG energy threshold required repeated manual testing to set, this the paper established a this mathematical model of continuous actions based on Gaussian sEMG energy curve, in which the energy threshold was set according to the distribution of Gaussian signal section, and differentiated the action signals from no-action signals combined with energy comparison method. The experiment results showed the method can achieve continuous repetitive action segmentation, and compared with manual segmentation of the same signal, has a higher degree of coincidence.
Algorithms ; Electromyography ; Humans ; Motion ; Pattern Recognition, Automated ; Signal Processing, Computer-Assisted

The canonical Wnt signaling pathway has been the hot spot in the signaling pathway studies which are related to ovary carcinogenesis in recent years,whose activation may induce the multiplication and self-renewal,survival and metastasis of ovarian cancer stem cells.The target gene mutations of key members in the canonical Wnt pathway and abnormal expresses of negative regulation molecules are closely associated with the occurrence and development of ovarian cancer.Deeply investigating the regulative mechanisms of canonical Wnt signaling pathway in ovarian cancer,and taking this as the therapeutic target to conduct the basic research and preclinical experiment may provide theoretical basis for treating better ovarian cancer patients in clinical practice.

Objective To assess the role of MR myocardial perfusion imaging (MRMPI) in detecting the viabilitive of myocardium in patient with old myocardial infarction (OMI) Methods MRMPI was performed in 47 patients suffering from old myocardial infarction with 1 5 T MR scanner Male 44, female 3, age ranged from 30 to 87 years with a mean of 61 years All patients were imaged during the first pass and delayed phase 5-30 min after injection of Gd DTPA 0 1 mmol/kg at 5 ml/s using IR turbo FLASH sequence Short axis images were acquired during the first pass, and both short axis and long axis images during delayed phase The left ventricular wall on short axis slice was divided into 8 segments A correlative study was carried out in 17 patients with the results of rest and stress 99m Tc SPECT scanning Results In 47 patients of old myocardial infarction, 29(61 7%) showed hypoenhancement during the first pass imaging (the SI was 50 20?7 45 percent of normal segment)and 46(97 9%) revealed delayed hyperenhancement(the SI was 491 0?197 26 percent of normal segment). For delayed hyperenhancement, 10(21 3%) was transmural ,15(31 9%) was nontransmural, and 21(44 7%) mixed, another one was normal 37 segments in 17 patients revealed to be nonviable by rest and stress 99m Tc SPECT, and the MRMPI all showed delayed hyperenhancement 116 viable segments on rest and stress 99m Tc SPECT, and no delayed hyperenhancement in 97 segments (83 6%) on MRMPI With the rest and stress 99m Tc SPECT as a standard of reference the sensitivity and the specificity of MRMPI were 100.0% and 83 6% respectively Conclusion The myocardial viability and nonviability as well as their severity and extent may be effectively evaluated with MRMPI in patients with OMI

Objective To observe the pulmonary pathologic changes of endotoxin (ET)-induced acute lung injury (ALI) in rabbits and the potential protective effects of fructose-1,6-diphosphate (FDP) on the ET-induced ALI of rabbits. Methods 24 flap-eared albation rabbits were randomly assigned to 3 groups, 8 for each, as follows: control group (group A), ET-treated group (group B) and combination group (treated by ET and FDP, group C). ALI was induced by injection of ET at one time. Group A was only injected with placebo, normal saline. ET was given to the rest groups. In group C, FDP was given as an intervening measure after rabbits injured. Rabbits were sacrificed at 6h time point. The pulmonary pathologic changes were observed. Some markers of pulmonary tissues, including the content of lipid peroxide (LPO), thromboxane B2 (TXB2), 6-keto-prostaglandin F1α(6-keto-PGF1α), interleukin-13 (IL-13) and the activity of superoxide dismutase (SOD), were observed. Results Compared with group A, the contents of LPO and TXB2 of group B showed significant increase (P＜0.05, P＜0.01), the SOD activity of group B weakened obviously (P＜0.01), the contents of 6-keto-PGF1α and IL-13 showed no statistical differences. The LPO content and the SOD activity of group C were similar to those of group A, the contents of TXB2, 6-keto-PGF1α and IL-13 of group C were much higher than those of group A (P＜0.01). Estimated by light microscope and electron microscope, the structure of lung tissue of group A is basically normal, the pathologic injuries of lung tissue of group B were much more severer and that of group C were slighter. Conclusion In the progress of ET-induced ALI, the oxidative injury, imbalance of TXB2/6-keto-PGF1α ratio and the secretion deficiency of protective cytokines play important role in inducing pathologic injuries of lung tissues. FDP can inhibit oxidative injury, ameliorate TXB2/6-keto-PGF1α balance and promote the secretion of protective cytokines, which, in turn, can protect rabbits from ET-induced ALI to some extent.

Objective To evaluate the effect of the integrated strategy in prevention and control of intestinal nematodiasis in Hongze County,so as to provide the evidence for formulating the control strategies and measures in the future. Methods Since 1995,the integrated strategy has been carried out for intestinal nematodiasis,and the measures included deworming,health ed?ucation,safe water,sanitation and environmental remediation. The effects of the integrated strategy were evaluated by the inves?tigations of the prevalence of soil?transmitted nematodiasis,awareness of health knowledge and behaviors of residents. Results From 1995 to 2014,601 900 person?times were administrated with deworming medication and the coverage rate of villages and towns was 100%. The benefit rate of safe water was 100%. The popularity rate of harmless toilets was 92.77%. The prevalence of intestinal nematodiasis decreased from 26.04%in 1995 to 0.56%in 2014,and the difference had statistical significance(χ2=693.54,P < 0.01). The awareness rate of health knowledge and correct rate of health behaviors increased from 43.13% and 40.94%in 1995 to 98.00%and 96.80%in 2012,respectively(χ2=181.97 and 182.14 respectively,P<0.01). Conclusion The prevalence of intestinal nematodiasis has been controlled effectively through the integrated strategy in Hongze County ,Jiang?su Province.

Objective To investigate the clinical and pathological features of small cell carcinoma of the urinary bladder. Methods The pathological and clinical data of 9 cases of small cell carcinoma were analyzed retrospectively. There were 6 males and 3 females, ages 45 to 79 years (mean age, 62 years). Clinical manifestations of 7 cases included gross hematuria and dysuria, the other 2 cases experienced lower abdominal pain. The mean tumor size was 2.0 cm (ranged, 0.5 to 7.0 cm). Two cases had multiple tumors and 5 cases had single tumors. The growth pattern in 2 cases was diffuse growth in the whole bladder. In 4 cases tumor cells were found in urine cytology. All 9 patients underwent surgical treatment, including TURBt. Four patients were diagnosed as superficial tumors before operation. All the patients underwent regular theprubicine irrigation in the bladder. One case underwent additional intravenous chemotherapy for 3 cycles. Partial cystectomy was performed in 2 cases, with regular theprubicine irrigation in bladder and 1 case underwent intravenous chemotherapy for 2 cycles. Radical cystectomy was performed in 3 cases, with 2 cases undergoing intravenous chemotherapy after operation. Results Pathological findings showed that tumor cells were small and round in shape. These hyperchromatic nuclei showed limited cytoplasm with lack of nesting characters. CgA and NSE were positive in immunohistochemistry. The final diagnosis was small cell carcinoma, with 1 case accompanied with transitional cell carcinoma and 1 case accompanied with prostate cancer. One case showed high preoperative serum calcium (3.15 mmol/L) and low serum phosphate (0.61 mmol/L), which returned to normal 1 month after operation. Four cases who′s bladder was preserved were followed up, 3 cases were alive for 4, 9 and 25 months after operation. The 1 case who underwent intravenous chemotherapy was followed up for 24 months and there was no sign of relapse or metastasis. In all the 3 cases with radical cystectomy, 2 cases died 2 and 28 months postoperativly. Another case with adjuvant chemotherapy was followed up for 24 months without recurrence or metastasis. Conclusions Small cell carcinoma of the urinary bladder is highly malignant with poor prognosis. Radical cystectomy in combination with systemic chemotherapy has better efficacy. Retained bladder surgery with systemic chemotherapy is an alternative choice. The most important factors which influence the prognosis of the tumor are clinical stage and therapeutic methods.

BACKGROUND: The important pathological changes of acute respiratory distress syndrome (ARDS) is disruption of the lung alveolar-capillary membrane barrier and resultant pulmonary edema associated with a proteinaceous alveolar exudate. Bone marrow mesenchymal stem cells (BMSCs) are able to carry on dividing and renewing themselves, and can eventually develop into many other types of cells. This provides a new treatment for treating injury of lungs.OBJECTIVE: To investigate the prevention of endotoxin-induced acute lung injury in rabbit by BMSCs.DESIGN, TIME AND SETTING: The randomized controlled animal study was performed at the Central Laboratory of Tangdu Hospital from October 2007 to January 2008.MATERIALS: A total of 20 rabbits were used in this study. Two rabbits were utilized to culture BMSCs. Eighteen rabbits were randomly assigned to three groups, saline control group, acute lung injury group and cell transplantation group (n = 6). Endotoxin was purchased from Sigma, USA.METHODS: Rabbit BMSCs were isolated and cultured by the Ficoll method. At the third passage, BMSCs were harvested for use.In the acute lung injury and call transplantation groups, endotoxin was infused into the trachea to establish models of acute lung injury/ARDS. Thirty minutes following model establishment, 2 mL BMSC suspension (1 x 105) was infused into the right jugular vein in the cell transplantation group. An equal volume of saline was injected into the saline control and acute lung injury groups.MAIN OUTCOME MEASURES: Number of neutrophilic granulocyte, wet to dry weight ratio of lung tissue, protein content and pathological changes in lung tissue in bronchoalveolar lavage fluid were measured.RESULTS: The increase in wet to dry weight ratio indicated the existence of pulmonary edema. The increase in neutrophilic granulocyte number suggested severe inflammatory reaction. The increased protein content showed the damage to lung alveolar-capillary membrane barrier. Following 48 hours of transplantation, neutrophilic granulocyte number and protein content in bronchoalveolar lavage fluid was significantly decreased (P < 0.01), and wet to dry weight ratio was significantly increased (P < 0.01) in the acute lung injury group compared with the saline control group. Compared with the acute lung injury group,neutrophilic granulocyte number and protein content was significantly increased (P < 0.01), and wet to dry weight ratio was significantly diminished (P < 0.01) in bronchoalveolar lavage fluid in the call transplantation group. Hematoxylin-eosin staining suggested that pulmonary alveoli was normal in the saline control group, presented typical acute lung injury in the acute lung injury group, and the pathological changes were mild in the cell transplantation group.CONCLUSION: BMSC transplantation can significantly reduce endotoxin-induced acute lung injury.

Objective To establish a mouse model of intrauterine malnutrition to investigate the growth characters and the DNA methylation level of H19 of the offspring. Methods The pregnancy ICR mice were randomly divided into the food restricted group and the control group. The food eaten by each pregnant mouse every day was monitored from d0. 5 to d12. 5. The food restricted pregnancy mice were given half amount food of the daily consumption during d0. 5-d12. 5. The mice in control group were given the normal food. The pregnant mice of both groups of were given normal food after delivery. The number, birth weight and sex ratio of the offspring were calculated. Furthermore, the body weight of the offspring was monitored at different growth stages. The oocytes of female offspring mice were obtained to detect the DNA methylation level of H19. Results (1) Compared to normal feeding pregnant mice, mice with food restricted were small and less active, and even some of them were died before delivery. The incidence of premature birth was significantly increased (P ＜0. 05). The number of abortion, stillbirth pregnant mice in the food restriction group were more than that in the control group, but the difference was not statistically significant (P ＞ 0. 05). (2) There was no significant difference in the number of offspring between pregnancy mice with food restricted or normal food (P ＞ 0. 05). As well as the number of male offspring between the two groups (P ＞ 0. 05). However, the offspring of the food restricted pregnant mice showed significant low birth weight (P ＜ 0. 05). (3) At the age of 3 weeks, there was still a low body weight in the offspring of the food restricted pregnant mice, but the difference had no significant difference between groups (P ＞ 0. 05). After weaning at 3 weeks, the offspring of food restricted pregnant mice began the catch-up growth. At the age of 6 weeks, the body weight of offspring of food restricted pregnant mice was higher than that of the normal offspring, but the difference was not statistically significant (P ＞ 0. 05). During the period of 6-8 weeks, the weight of offspring of normal feed pregnancy mice was increased slowly. But the body weight of food restriction offspring mice was increased rapidly. At the age of 8 weeks, the body weight of offspring of food restricted group was significantly higher than that in control group (P ＜ 0. 05). (4) The methylation level of H19 in oocyte from offspring of food restricted pregnancy mice was normal. Conclusion Food restriction at pregnancy period not only affect the outcome of pregnancy, but also increased the rate of premature birth. The offspring of food restriction pregnancy mice showed low birth weight. Until the age of 6 weeks, the offspring of food restriction pregnancy mice had lower body weight compared with the offspring with normal feeding pregnancy mice. The offspring of food restriction pregnancy mice demonstrated obesity at 8 weeks. But the H19 methylation levels of oocytes from offspring of pregnancy mice with food restriction was normal.