Exercise ; Heart Arrest ; Humans

Objective: To study the predictive value of 12-lead electrocardiogram (ECG) abnormality on prognosis of chronic heart failure in patients with dilated cardiomyopathy (DCM-CHF).
Methods: A prospective, multicenter follow-up study in 787 DCM-CHF patients was conducted, and the endpoints were obtained by clinical visit, mail contact and telephone conversation. The independent predictors for all cause death were determined by Cox regression analysis, QRS duration > 120 ms was studied and the survival rates were investigated by Kaplan-Meier analysis.
Results: There were 203 patients died during the follow-up period. Cox regression analysis found that the following indexes were related to all cause death: atrial fibrillation (AF) (HR=2.064, 95% CI 1.102-3.864,P<0.05), non-sustained ventricular tachycardia (NSVT) (HR=3.887, 95% CI 1.554-9.724,P<0.05) and QRS duration (HR=1.010, 95% CI 1.002-1.018, P<0.05). Kaplan-Meier analysis revealed that the survival rates were different by each stratiifcation of QRS duration,P<0.05.
Conclusion: ECG indexes of AF, NSVT and QRS duration had the important impact on the survival rate in DCM-CHF patients; there were signiifcant differences between QRS durations and survival rates.

Objective Observing the effect of Shuganyishen formula on hormone-dependant metastatic breast cancer and the mechanisms of anti-resistance and endocrine therapy. Methods 226 ITT cases were divided into two cohorts by treatment. 126 cases were treated with the combination of Shuganyishen formula and standard western medicine including endocrine therapy, and 100 cases were treated with western medicine alone. The end points were time to progress (TTP) and overall survival and quality of life (QOF). The effect of Shuganyishen formula on growth of TAM-resistance cell line in vitro was observed. Results There were statistical significances of median TTP between combination and western medicine in Luminal A (ER+ /PR+, HER2-) as well as Luminal B (ER+/PR+, HER2+), but no significances of OS; The extent and incidence of hot flush, sweat and syndromes related to bone were decreased significantly in combination treated group. Shuganyishen formula could reverse resistance to TAM. Conclusion Patients with LuminalA and LuminalB metastatic breast cancer benefit from Shuganyishen formula, which maybe related with its reverse resistance to endocrine therapy.

Objective: To assess the impact of viable myocardium in left ventricular aneurysm (LVA) and ventricular arrhythmia on prognosis of LVA patients.
Methods: A total of one hundred and sixty LVA patients who received99Tcm-MIBI SPECT and18F-FDG PET were enrolled, including 139 male and 21 female with the mean age of (58 ± 10) years.There were 42 (26.3%) patients combining ventricular arrhythmia. LVEDV, LVESV and LVEF were detected. Semi-quantitative analysis of myocardium perfusion imaging was conducted, viable myocardium in aneurysm was deifned as the perfusion-metabolism mismatch score (MMS) ≥ 2.0. According to myocardium viability, the patients were divided into 2 groups: No viability group,n=97 and With viability group,n=63;based on ventricular arrhythmia, the patients were divided into another 4 groups: Group①, viability-, ventricular arrhythmia-, n=68, Group②, viability-, ventricular arrhythmias+,n=29, Group③, viability+, ventricular arrhythmias-,n=50 and Group④, viability+,ventricular arrhythmias+,n=13. The average follow-up time was (50 ± 7) months, the end point was cardiac death. The survival curve was obtained by Kaplan-Meier method and survival rates were compared by Log-rank analysis.
Results: The mean LVEF in 160 patients was (34 ± 11) %, cardiac death occurred in 19 (11.9%) patients. Long-term survival rates in Groups①,② and③ were 94.1%, 89.7% and 86.0%, respectively,P>0.05; while in Group④, the survival rate was 61.5%, which was lower than the other 3 groups,P=0.004. Multivariate Cox regression analysis showed that female (HR=5.101, 95% CI 1.853-14.044, P=0.002), GPET-ESV (HR=1.009, 95% CI 1.002-1.015,P=0.013), interaction between MMS and ventricular arrhythmia (HR=1.368, 95%CI 1.113-1.681,P=0.003) were independent risk factors for cardiac death;while surgical treatment (HR=0.199, 95% CI 0.054-0.742,P=0.016) could decrease the risk of cardiac death.
Conclusion: Patients with viable aneurysm and ventricular arrhythmia had poor long-term prognosis; while early and active treatment is needed for them (surgery with anti-arrhythmic therapy).

Objective To investigate the effect of c?Met inhibitor AMG?102 on proliferation and radiosensitivity in laryngeal squamous carcinoma cells. Methods The effects of AMG?102 on proliferation and radiosensitivity of laryngeal squamous carcinoma cell lines Hep?2 and KBV200 were detected by 3?(4,5?dimethy?2?thiazolyl)?2, 5?diphenyl?2H tetrazolium bromide ( MTT ) assay and colony formation assay, respectively. The apoptosis of Hep?2 and KBV200 cells was detected by flow cytometry.The expression levels of c?Met, phospho?Met (p?Met), cleaved caspase?3 and Akt／p?Akt, Erk／p?Erk were detected by Western blot. Specific small interfering RNA targeting c?Met or plasmid of c?Met were transfected into Hep?2 and KBV200 cells to investigate the cell sensitivity to AMG?102. Results Compared with KBV200 cells, Hep?2 cells were more sensitive to AMG?102 with IC50 of 14 and 9 μmol／L, respectively. The relative expression levels of c?Met and p?Met proteins in Hep?2 cells were 194.48±0.57 and 177.76±1.53, respectively, which were significantly higher than those in KBV200 cells ( 171.24 ± 1.00 and 115.37 ± 0.56, respectively, P＜0.001 for both). Exogenous hepatocyte growth factor (HGF) was added to increase the expression level of p?Met protein in KBV200 cells.The results showed that AMG?102 significantly reduced the expression of p?Met in KBV200 cells treated with HGF ( P＜0.001). Compared with the dimethyl sulfoxide ( DMSO) group, AMG?102 treatment combined with radiotherapy significantly increased the radiosensitivity of Hep?2 cells ( SER＝1.28, P＜0.001). However, AMG?102 had little effect on the radiosensitivity of KBV200 cells (SER＝1.18, P＝0.002). Compared with the 4 Gy radiotherapy alone group and the 5 μmol／L of AMG?102 alone treatment group, the apoptosis rate of Hep?2 cells in the combined treatment group was significantly increased. Meanwhile, the expression level of cleaved caspase?3 protein was also markedly increased. However, there were no significant changes in the apoptotic rate and cleaved caspase?3 expression in each treatment group of KBV200 cells. Compared with DMSO treatment group, the expression levels of p?Met, p?Akt and p?Erk were significantly decreased in the 4 Gy radiotherapy group, 5 μmol／L of AMG?102 treatment group and combined treatment group of Hep?2 cells. And the levels of p?Met, p?Akt and p?Erk in the combined treatment group were significantly lower than those in the 4 Gy radiotherapy alone group and 5 μmol／L of AMG?102 treatment alone group. By contrast, in KBV200 cells, the expression of p?Met, p?Akt and p?Erk in each group was not changed. The relative expression of p?Met in Hep?2 cells before and after radiotherapy at 30 min, 1 h, 4 h, 8 h, 24 h were 99.89±0.61, 138.62±1.00, 163.07±5.00, 87.80±1.85, 90.67±0.65 and 94.09±1.41, respectively. The level of p?Met was slightly increased after radiotherapy at 30 min and 1 h (P＜0.001 for all), whereas it was significantly decreased from 4 h to 24 h after radiotherapy (P＜0.05 for all). By contrast, the expression of p?Met in KBV200 cells did not change with time after radiotherapy (P＞0.05). The sensitivity of Hep?2 cells to AMG?102 was decreased after silencing of c?Met, while the sensitivity of KBV200 cells to AMG?102 was not significantly changed ( P＞0.05). Moreover, the radiosensitivity of Hep?2 cells in c?Met knockdown group had a slightly increasing trend ( SER＝1.07, P＝0.068). After the treatment with 10 μmol／L of AMG?102, the proliferation rate of c?Met ectopically expressed KBV200 cells was 60.05%± 3.23%, It was significantly lower than that of the blank control 90.08%±1.04% and siRNA negative control (90.12%±1.01%, P＜0.001). The results suggested that the overexpression of c?Met in KBV200 cells increased the radiosensitivity to AMG?102, whereas depletion of c?Met resulted in resistance to AMG?102 in Hep?2 cells. Furthermore, the radiosensitivity of KBV200 cells that overexpressed c?Met showed a decreased trend (SER＝0.7, P＝0.005). Conclusions c?Met inhibitor AMG?102 has a significant inhibitory effect on the proliferation of c?Met overexpressing laryngeal squamous carcinoma cells, leading to increased radiosensitivity. It suggests that molecular targeted therapy against c?Met receptor is more effective in c?Met overexpressed subtype of laryngeal squamous cell carcinoma.

Objective To investigate the effect of c?Met inhibitor AMG?102 on proliferation and radiosensitivity in laryngeal squamous carcinoma cells. Methods The effects of AMG?102 on proliferation and radiosensitivity of laryngeal squamous carcinoma cell lines Hep?2 and KBV200 were detected by 3?(4,5?dimethy?2?thiazolyl)?2, 5?diphenyl?2H tetrazolium bromide ( MTT ) assay and colony formation assay, respectively. The apoptosis of Hep?2 and KBV200 cells was detected by flow cytometry.The expression levels of c?Met, phospho?Met (p?Met), cleaved caspase?3 and Akt／p?Akt, Erk／p?Erk were detected by Western blot. Specific small interfering RNA targeting c?Met or plasmid of c?Met were transfected into Hep?2 and KBV200 cells to investigate the cell sensitivity to AMG?102. Results Compared with KBV200 cells, Hep?2 cells were more sensitive to AMG?102 with IC50 of 14 and 9 μmol／L, respectively. The relative expression levels of c?Met and p?Met proteins in Hep?2 cells were 194.48±0.57 and 177.76±1.53, respectively, which were significantly higher than those in KBV200 cells ( 171.24 ± 1.00 and 115.37 ± 0.56, respectively, P＜0.001 for both). Exogenous hepatocyte growth factor (HGF) was added to increase the expression level of p?Met protein in KBV200 cells.The results showed that AMG?102 significantly reduced the expression of p?Met in KBV200 cells treated with HGF ( P＜0.001). Compared with the dimethyl sulfoxide ( DMSO) group, AMG?102 treatment combined with radiotherapy significantly increased the radiosensitivity of Hep?2 cells ( SER＝1.28, P＜0.001). However, AMG?102 had little effect on the radiosensitivity of KBV200 cells (SER＝1.18, P＝0.002). Compared with the 4 Gy radiotherapy alone group and the 5 μmol／L of AMG?102 alone treatment group, the apoptosis rate of Hep?2 cells in the combined treatment group was significantly increased. Meanwhile, the expression level of cleaved caspase?3 protein was also markedly increased. However, there were no significant changes in the apoptotic rate and cleaved caspase?3 expression in each treatment group of KBV200 cells. Compared with DMSO treatment group, the expression levels of p?Met, p?Akt and p?Erk were significantly decreased in the 4 Gy radiotherapy group, 5 μmol／L of AMG?102 treatment group and combined treatment group of Hep?2 cells. And the levels of p?Met, p?Akt and p?Erk in the combined treatment group were significantly lower than those in the 4 Gy radiotherapy alone group and 5 μmol／L of AMG?102 treatment alone group. By contrast, in KBV200 cells, the expression of p?Met, p?Akt and p?Erk in each group was not changed. The relative expression of p?Met in Hep?2 cells before and after radiotherapy at 30 min, 1 h, 4 h, 8 h, 24 h were 99.89±0.61, 138.62±1.00, 163.07±5.00, 87.80±1.85, 90.67±0.65 and 94.09±1.41, respectively. The level of p?Met was slightly increased after radiotherapy at 30 min and 1 h (P＜0.001 for all), whereas it was significantly decreased from 4 h to 24 h after radiotherapy (P＜0.05 for all). By contrast, the expression of p?Met in KBV200 cells did not change with time after radiotherapy (P＞0.05). The sensitivity of Hep?2 cells to AMG?102 was decreased after silencing of c?Met, while the sensitivity of KBV200 cells to AMG?102 was not significantly changed ( P＞0.05). Moreover, the radiosensitivity of Hep?2 cells in c?Met knockdown group had a slightly increasing trend ( SER＝1.07, P＝0.068). After the treatment with 10 μmol／L of AMG?102, the proliferation rate of c?Met ectopically expressed KBV200 cells was 60.05%± 3.23%, It was significantly lower than that of the blank control 90.08%±1.04% and siRNA negative control (90.12%±1.01%, P＜0.001). The results suggested that the overexpression of c?Met in KBV200 cells increased the radiosensitivity to AMG?102, whereas depletion of c?Met resulted in resistance to AMG?102 in Hep?2 cells. Furthermore, the radiosensitivity of KBV200 cells that overexpressed c?Met showed a decreased trend (SER＝0.7, P＝0.005). Conclusions c?Met inhibitor AMG?102 has a significant inhibitory effect on the proliferation of c?Met overexpressing laryngeal squamous carcinoma cells, leading to increased radiosensitivity. It suggests that molecular targeted therapy against c?Met receptor is more effective in c?Met overexpressed subtype of laryngeal squamous cell carcinoma.

Fusobacterium nucleatum (Fn) is an oral anaerobic bacterium that has recently been found to colonize on the surface of colorectal cancer cells in humans, and its degree of enrichment is highly negatively correlated with the prognosis of tumor treatment. Numerous studies have shown that Fn is involved in the occurrence and development of colorectal cancer (CRC), and Fn interacts with multiple components in the tumor microenvironment to increase tumor resistance. In recent years, researchers have begun using nanomedicine to inhibit Fn's proliferation at the tumor site or directly target Fn to treat CRC. This review summarizes the mechanism of Fn in promoting CRC and the latest research progress on Fn-related CRC therapy using different nanomaterials. Finally, the applications perspective of nanomaterials in Fn-promoted CRC therapy was prospected.
Humans ; Colorectal Neoplasms/pathology* ; Fusobacterium nucleatum/genetics* ; Base Composition ; RNA, Ribosomal, 16S ; Phylogeny ; Sequence Analysis, DNA ; Tumor Microenvironment

Objective:To understand the current status of personal protection in occupational population at high risk for brucellosis in China and provide evidence for the evaluation of implementation of National Brucellosis Prevention and Control Plan (2016-2020).Methods:Four counties in Shanxi Province and Xinjiang Uygur Autonomous Region were selected to conduct a questionnaire survey in occupational population at high risk for brucellosis from December 2019 to July 2020 by using cross-sectional survey methods.Results:A total of 2 384 persons at high risk for brucellosis were surveyed, and the standardized utilization rate of personal protective equipment (PPE) was 20.13% (480/2 384). The utilization rate of glove, mask, rubber shoe, and work cloth were 38.26% (912/2 384), 31.80% (758/2 384), 32.01% (763/2 384) and 30.87% (736/2 384),respectively. There were significant differences in the utilization rate and standardized utilization rate of the four types of PPE among populations in different age, occupation, educational level and area groups (all P<0.001). The utilization rate and standardized utilization rate of PPE were lower in people over 60 years old, women, farmers, and those with lower educational level. The results of multivariate analysis showed that occupation and area were the influencing factors for the standardized utilization of PPE, the standardized utilization rates of PPE were higher in herdsmen and veterinarians. The standardized utilization rate of PPE in Yanggao County and Huocheng County was significantly higher than that in Zuoyun County and Hunyuan County. Conclusions:The utilization rate of the four types of PPE in occupational population at high risk for brucellosis was not high in China, and the standardized utilization rate was low, lower than the requirement in National Brucellosis Prevention and Control Plan, and there were significant differences among different areas. It is urgent to distribute PPE to occupational population at high risk for brucellosis and carry out health education about PPE utilization. Meanwhile, it is necessary to strengthen information exchange or sharing among different areas.

Objective:To understand the current status of knowledge of brucellosis prevention and control among occupational groups at high-risk of brucellosis, and to provide a scientific basis for assessing the effectiveness of brucellosis prevention and control in China.Methods:A total of four counties in Shanxi Province and Xinjiang Uygur Autonomous Region were selected as survey counties from 2019 to 2020, and 600 people from the occupational groups at high-risk of brucellosis in each survey county were selected as survey respondents, and basic information and knowledge of prevention and treatment were collected through questionnaires. Single-factor and multi-factor logistic regression models were used to analyze the factors affecting the population's knowledge of prevention and treatment.Results:A total of 2 411 people participated in the survey and 2 384 valid questionnaires were obtained, including 1 405 males and 979 females, with the youngest age being 18 years old, the oldest being 91 years old, and the median being 57 years old. The overall knowledge of brucellosis prevention and control was 17.74% (423/2 384). The knowledge rate was lower among people over 60 years old, farmers, and people with less than elementary school education (13.99%, 14.50%, and 13.78%), and higher among women, herders, and people with elementary school education (20.02%, 36.33%, and 19.58%); the knowledge rate was lower in Hunyuan County (0.51%), and the differences in overall knowledge rates by age, occupation, education level, and region were statistically significant (χ 2 = 18.25, 87.18, 11.05, 197.43, P < 0.001). Multi-factor logistic regression analysis showed that gender, occupation, literacy and region were associated with knowledge of prevention and treatment ( P < 0.05). Conclusions:The overall knowledge of prevention and treatment among high-risk occupational groups in the key areas of China's brucellosis prevention and treatment program is low, with a large gap with the goals of the national brucellosis prevention and treatment program, and gender, occupation, literacy level, and region are the influencing factors of the knowledge of prevention and treatment. There is an urgent need to carry out a variety of health education activities for high-risk occupational groups and to strengthen the exchange of experience on brucellosis prevention and treatment between regions.

Objective: To investigate the effects of c-Met inhibitor AMG-102 on the proliferation and apoptosis of laryngeal squamous carcinoma Hep-2 cells and the underlying mechanism. Methods: Laryngeal squamous carcinoma cell line Hep-2 cells were treated with 2.5, 5 and 10 μmol/L AMG-102, respectively. The proliferation activities of Hep-2 cells were detected by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT). The apoptotic rate of Hep-2 cells was detected by flow cytometry analysis and Hoechst staining. The mRNA expression levels of apoptosis-related genes were detected by real-time quantitative polymerase Chain reaction (RT-qPCR), and the protein expressions of c-Met/PI3K/AKT pathway were detected by western blot. Results: Compared with the control group, the proliferation rates of Hep-2 cells treated with 2.5, 5 and 10 μmol/L AMG-102 for 24 hours were (89.8±1.1)%, (79.8±1.0)% and (69.1±1.2)%, respectively; for 48 hours were (76.8±2.0)%, (60.2±1.1)% and (49.8±1.2)%, respectively; for 72 hours were (50.1±2.0)%, (41.5±1.1)% and (33.6±1.0), respectively, with significant differences (all P<0.05). The apoptotic rates of Hep-2 cells treated with 2.5, 5 and 10 μmol/L AMG-102 for 48 hours were (16.09±1.53)%, (27.51±2.02)% and (36.57±1.42)%, respectively, which were significantly higher than (3.62±0.10) % in the control group (all P<0.05). After treated with 2.5, 5 and 10 μmol/L AMG-102 for 48 hours, the relative expression levels of Bcl-2 mRNA in Hep-2 cells were 0.58±0.13, 0.38±0.12 and 0.20±0.13, respectively; the relative protein expression of p-Met were 80.0±3.8, 50.6±4.2 and 28.5±1.3, respectively; the relative protein expression of p-PI3K were 87.1±0.9, 54.2±1.2 and 21.0±1.2, respectively; the relative protein expression of p-AKT were 98.7±5.6, 56.9±3.2 and 32.2±4.3, respectively; which were significantly lower than those in the control group (all P<0.05). The relative expression levels of Bax mRNA were 1.78±0.13, 2.37±0.14 and 3.05±0.13, respectively, and the relative expression levels of caspase-3 mRNA were 1.98±0.14, 2.47±0.14 and 3.15±0.13, respectively, which were significantly higher than those in the control group (all P<0.05). Conclusion c-Met inhibitor AMG-102 could inhibit the proliferation and induce apoptosis of laryngeal squamous carcinoma Hep-2 cells by regulating the c-Met/PI3K/Akt pathway.