Objective To investigate online dating status and human immunodeficiency virus (HIV)infection among men who have sex with men (MSM)in Chongqing,and provide basis for effective intervention in HIV infec-tion among MSM.Methods 206 MSM in Chongqing were performed structured interview and questionnaire survey, MSM who didn’t conduct HIV testing within half a year were performed laboratory screening,sociological popula-tion characteristics and influencing factors of HIV in MSM were analyzed.Results The average age of 206 MSM was (22.08±1 .81)years old,137 (66.50%)received college education and above,HIV infection rate was 21 .36%(44/206).HIV infection rate in MSM who had no fixed partners was higher than those who had fixed partners;the more sexual partners they had,the greater the risk of HIV infection they got;HIV infection rate in MSM who had sex with unfamiliar persons or strangers was higher than those who had sex with familiar partners;the higher rate of condom use in sexual behavior,the lower rate of HIV infection was,there were significant differences among the groups(all P <0.05).Condom use among MSM with different educational levels was significantly different(all P <0.05).Conclusion HIV infection rate in MSM is high in this city,it is necessary to strengthen education about health,network partner,and behavioral intervention,reduce the bridge population connecting MSM network,es-tablish social support system of homosexuality,and reduce the risk of HIV infection in MSM.

Objective To investigate the clinical efficacy and safety of combined acupuncture and medicine in treating polycystic ovarian syndrome infertility.Methods Ninety patients with polycystic ovarian syndrome infertility were randomly allocated to treatment and control groups, 45 cases each. The control group received oral administration of clomifene citrate tablets plus muscular injection of chorionic gonadotropin and the treatment group, electroacupuncture in addition. The ovulation rates, pregnancy rates, incidences of ovarian hyperstimulation syndrome (OHSS), ectopic pregnancy rates and early miscarriage rates were compared between the two groups of patients. Results The ovulation rate and the pregnancy rate were 97.8 % and 37.8%, respectively, in the treatment group and 75.6% and 17.8%, respectively, in the control group; there was a statistically significant difference between the two groups (P<0.05). Of 17 pregnant patients in the treatment group, 2 had early miscarriage, accounting for 11.8% and none had ectopic pregnancy, accounting for 0.0%. Of 8 pregnant patients in the control group, 4 had early miscarriage, accounting for 50.0% and none had ectopic pregnancy, accounting for 0.0%. The incidence of OHSS was 4.4% in the treatment group and 6.7% in the control group; there was no statistically significant difference between the two groups (P>0.05).Conclusions Combined use of acupuncture and medicine can increase the ovulation rate and the pregnancy rate in patients with polycystic ovarian syndrome infertility. It does not significantly cause ovarian hyperstimulation and ectopic pregnancy and may decrease the early miscarriage rate.

Objective To examine the current status of the sexual behavior among out‐of‐school adolescent men who have sex with men(MSM)and analyze the influential factors of AIDS infection in order to provide countermeasures.Methods The software SPSS 16.0 was used for cross table analysis and chi square test of questionnaire data and HIV detection data.Results The HIV infection rate was 21.36%.The household registration ,the only child ,living state ,coming out of the closet ,fixed sex‐ual partner ,the way they know sexual partners had no significant effects on HIV infection(P>0.05).Having multiple sexual partners(P<0.05) ,unfamiliar with sexual partners(P<0.05) ,having sex with network friends(P<0.05) ,no condom use in sexual life(P<0.01) ,having no regular HIV testing(P<0.01)were significantly related to HIV infection.Conclusion Reduc‐ing the sexual behavior among out‐of‐school adolescent MSM ,carrying out HIV counseling and testing ,promoting the use of condoms contribute to the reduction of HIV infection.

This study is aimed to evaluate the clinical application of the millimeter wave and magnetism light compound therapy. The EHF-98B MMW. RL compound therapy apparatus made in the University of Electronic Technology(Chengdu) was used in 171 patients. The superficial, skin lesions or the visceral reflected skin regions (acupoints) were directly exposed to the light from the apparatus. All the cases were divided into five groups, namely skin mucosa superficial lesions, trauma of the bone and joint soft tissue, surgical incision, ENT infections, and rare intricate diseases. The therapeutic effects observed in the groups were analyzed and evaluated by means of 4 levels. As for the 171 patients, the cure rate was 42.7% (73 patients), the effective rate 25.1%(43 patients), the improvement rate 31%(53 patients), and no effect constituted 1.2%(2 patients). The total effective rate was 98.8%. This therapy was especially effective for treating chronic cervicitis, cervical erosion, soft tissue trauma, surgical incision. Also it was effective for treating some rare intricate diseases, e.g. sterility, vitiligo, Behcet disease. So the millimeter wave and magnetism light compound therapy may find wide clinical applications.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Combined Modality Therapy ; Female ; Humans ; Infant ; Infant, Newborn ; Joint Diseases ; therapy ; Magnetics ; therapeutic use ; Male ; Microwaves ; therapeutic use ; Middle Aged ; Otorhinolaryngologic Diseases ; therapy ; Phototherapy ; Skin Diseases, Infectious ; therapy ; Soft Tissue Injuries ; therapy

Objective To analyze the clinical characteristics of epileptic children at early stage,and to explore the high risk factors for children′s refractory epilepsy(RE)in order to provide evidence for the early and timely treatment of RE.Methods A total of 147 epileptic patients with correct diagnosis and reasonable treatment were enrolled.Forty-nine patients were classified as drug non-responsive epilepsy(DNR-EP group).The remaining 98 patients were classified as drug-responsive epilepsy(DR-EP group).With multiple conditional Logistic regression,the clinical characte-ristics between the 2 groups were compared to identify the early predictors for RE.Results Single factor Logistic regression analysis showed that the initial age of onset <1 year,more than 20 seizures before treatment in a week,focal seizures,more than 2 kinds of epileptic seizures,changes in seizure type in the course of disease,neurological impairment,early intellectual disability,abnormal cranial magnetic resonance imaging(MRI),fixed focal abnormalities on video electroencephalogram(VEEG) after seizure-free interval,diffused anomaly of non-epileptic wave in VEEG before treatment,poor response to initial antiepileptic drugs(AEDs) therapy,compliance with the characteristics of epileptic encephalopathy at early stage significantly increased in DNR-EP group.Then multivariate conditional Logistic regression analysis demonstrated that more than 20 seizures before treatment in a week(OR=2.679,P=0.043),more than 2 kinds of epileptic seizures(OR=4.364,P=0.013),fixed focal abnormalities on VEEG after seizure-free interval(OR=3.898,P=0.008),poor response to initial AEDs therapy(OR=2.611,P=0.036),and compliance with the characteristics of epileptic encephalopathy at early stage(OR=6.022,P=0.002) were the risk factors for developing into RE.Conclusions Children are likely to develop into RE if they have more than 20 seizures before treatment in a week,with more than 2 kinds of epileptic seizures,fixed focal abnormalities on VEEG after seizure-free intervals,poor response to initial AEDs therapy,and compliance with the characteristics of epileptic encephalopathy at early stage.

Blood oxygen saturation is one of the important index of clinical care. In this paper, a pulse oximetry method based on android smartphone was proposed, by which oxygen saturation could be monitored anywhere. The non-intrusive reflective optical measuring principle was used in the new method. After the sampled images were analyzed and processed, value of blood oxygen saturation was calculated in real time. The experimental results show that the method has high reliability and consistency.

Objective:To analyze the clinical characteristics of three patients with Immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome.Methods:Three patients with IPEX syndrome diagnosed at the Children′s Hospital of Fudan University from January 24, 2013 to July 29, 2019 were selected as the study subjects. Their clinical features, laboratory investigations and results of genetic testing were summarized. Treatment and prognosis were also explored.Results:All of the three children had developed the disorder during infancy. One child had initial features including diabetes and diabetic ketoacidosis, whilst the other two had initiated by diarrhea. All patients had gastrointestinal involvement, and one was diagnosed as very early onset inflammatory bowel disease by colonoscopy and biopsy. Two children also had endocrine glands involvement. One child had manifested type 1 diabetes and positivity for thyroglobulin and thyroid peroxidase antibodies, though his thyroid function had remained normal. Another one had hypothyroidism and was treated by levothyroxine. Genetic testing revealed that all children had harbored missense variants of the FOXP3 gene, including c. 1222G>A (p.V408M), c. 767T>C (p.M256T) and c. 1021A>G (p.T341A). The clinical symptoms of one patient were alleviated following allogeneic hematopoietic stem cell transplantation. One patient was stable after treatment with infliximab plus insulin, and one child had died of refractory septic shock and multiple organ dysfunction syndrome at 3 months old. Conclusion:FOXP3 gene variant-associated IPEX syndrome may have very early onset and diverse clinical manifestations. For male patients with infantile onset chronic diarrhea, multiple endocrine or multiple system involvement, genetic testing is recommended, which may facilitate early diagnosis, treatment and genetic counseling.

Objective:To evaluate the dry eye status of nursing school students and understand their ocular surface health.Methods:Using the convenience sampling method, a total of 90 nursing students studying in the Nursing College of Capital Medical University in March 2018 were selected as the research objects. The OCULUS Jenvis-OSDI dry eye questionnaire and OCULUS ocular surface comprehensive analyzer were used subjectively and objectively to analyze the heath of ocular surface.Results:According to the OSDI dry eye questionnaire, mild dry eyes were 21 cases accounted for 23.3%, moderate dry eyes were 42 cases accounted for 46.7%, and severe dry eyes were 21 cases accounted for 23.3%. The main symptom of dry eye was foreign body sensation, accounting for 53.3%. The secondary symptoms were photophobia and blurred vision, which accounted for 46.7% and 40%, respectively. The OCULUS ocular surface comprehensive analyzer showed that the amount of tear secretion in both eyes was normal. In dry eye grading based on average tear film break-up time, critical dry eye accounted for about 60% and dry eye accounted for about 10%. Eye red analysis showed that 9 patients (10%) with hyperemia score>2.0 were dry eye caused by inflammation.Conclusions:The nursing school students have a high incidence of dry eye and subjective symptoms. It indicates that the students overuse eye and do not pay attention to eye hygiene. It is the responsibility of the nursing teacher to carry out health education for students, popularize scientific and rational use of eye knowledge, and pay attention to eye health.

Objective: To explore the relationship between gap junction and glucose uptake of astrocytes under oxygen-glucose deprivation(OGD) and reperfusion. Methods: Cerebral cortical astrocyte from 1 day newborn SD rats were undergone the primary culture. The ischemia cell model was established by OGD. This experiment were divided into control group, OGD group and OGD+ CBX group.After different reperfusion time (0 h, 12 h 24 h and 48 h), the glucose uptake of astrocyte was measured by 2-NBDG through flow cytometry analysis and connexin 43(Cx43) gap junction plaques was detected using immunofluorescene. Results: Compared with the control group, the glucose uptake of astrocyte was up-regulated induced by OGD following different reperfusion time.The glucose uptake of OGD group was (2.32±0.43)nmol/μgDNA in 24 hours reperfusion time and was (0.95±0.28)nmol/μgDNA in control group. The up-regulation was up to 2.63-fold increase (t=13.99, P=0.0024) in 24 hours after reperfusion.Compared with the control group, the Cx43 gap junction number was up to 2.5- fold increase(t=11.34, P=0.003) and the size was 1.85-fold increase (t=10.27, P=0.004) in 24 h reperfusion. The glucose uptake of astrocyte after OGD was reduced by CBX and the decrease was 42% in 48 h after reperfusion. Conclusion Those results urges us consider the clinical treatment for interfering with Cx43 gap junction.