Objective To establish the quantitative transfusion of hemoglobin method and explore the application of this method in neonatal transfusion.Methods This study selected 93 cases of anemic neonates who had no other underlying diseases from the Neonatal Department of Maternal and Child Health (MCH) in Dalian,and they were the principle of weight and age divided into two groups.The first group (control group:conventional method) was transfused and injected the red blood cells that were leukocytereduced,irradiated and washed twice by 0.15 U/kg under the normal reservation.The study measured the hemoglobin value before transfusion and within 24 h after being transfused,and then detected the hemoglobin,volume,and hematocrit of a small amount of blood during the preparation,and finally calculated the utilization rate of hemoglobin in neonates who were transfused.For the second group (observation group:quantitative erythrocyte injection of hemoglobin),it calculated in reverse how much hemoglobin the children needed to be supplemented,based on the doctor's expected hemoglobin values achieved after children being transfused and the utilization rate of hemoglobin obtained from the first group.Then,according to the calculation,it prepared a small amount of blood of the red blood cells that were leukocyte-reduced,irradiated and washed,made the quantitative injection of hemoglobin twice,and measured the hemoglobin value before transfusion and within 24 h after being transfused.At last,it analyzed statistically the results.Results Both two methods effectively improved the children's anemia (P ＜ 0.05).There was no difference (P ＞ 0.05) for two methods in improving the anemia of children,but the quantitative transfusion of hemoglobin (s =6.6,cv =4.6％) could basically reach the doctor's expected hemoglobin value,and it avoided the situations in the first method (s =14.45,vc =8.6％),like that after transfusion a minority of hemoglobin was too high or it did not reach complete amelioration of anemia of the children.Conclusions Both traditional transfusion method and quantitative transfusion of hemoglobin method can improve the anemia of the children,but the latter one is more suitable for the clinical needs and achieves the true quantitative transfusion.